Molecular characterization and prevalence of cystic echinococcosis in slaughtered water buffaloes in Turkey

Beyhan, Yunus Emre; Umur, Şinasi

doi:10.1016/j.vetpar.2011.04.038

Cited by 44 publications

(33 citation statements)

References 23 publications

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“…The results of necropsy of cattle showed that 21 cows (19%) were infected with CE and fertile cysts were found in liver, lungs and both of organs of 8 (38%) heads. It should be noted that the lungs were the predominant sites of the CE in cattle as already reported (Beyhan and Umur 2011).…”

Section: Discussionsupporting

confidence: 62%

Serological diagnosis of cystic echinococcosis in cattle

Bulashev¹,

Suranshiev²,

Akibekov³

et al. 2017

FOLIA PARASIT

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An IgM murine monoclonal antibody (MAb) was obtained against the excretory-secretory antigen (ES-Ag) of in vitro reared protoscoleces of Echinococcus granulosus (Batsch, 1786). Western blotting revealed that the MAb recognised a 20.6 kDa protein of this ES-Ag. The MAb was used in sandwich enzyme-linked immunosorbent assay (s-ELISA) for selective sensitisation of the solid phase with the protoscolex-specific protein from its ES-Ag and somatic antigen (S-Ag) to examine serum samples of 108 cows from a cystic echinococcosis (CE) endemic area for specific antibodies and to compare the results with those from necropsies and an indirect ELISA (i-ELISA). The sensitivity of s-ELISA/ES-Ag, s-ELISA/S-Ag and i-ELISA/S-Ag was 48%, 52% and 62%, respectively. The low sensitivity of the ELISA was probably caused by the fact that 13 cows (62%) were infected with sterile cysts (acephalocysts and/or calcified foci) only. A relatively high specificity (80%) of s-ELISA/ES-Ag was observed in cows with fertile cysts. It also detected antibodies in the serum of two cows that had recovered from the disease according to the necropsy. The i-ELISA/S-Ag gave false results in testing sera from a healthy animal and from a cow with tubercular foci. Further analysis will be necessary to define more precisely the value of this study, because the duration of antibody elimination from the bloodstream of recovered cattle remains unknown. The solution of this problem will increase the specificity of the proposed test in monitoring herbivorous animals for CE.

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Section: Discussionsupporting

confidence: 62%

Serological diagnosis of cystic echinococcosis in cattle

Bulashev¹,

Suranshiev²,

Akibekov³

et al. 2017

FOLIA PARASIT

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“…The investigators used different methods to classify E. granulosus isolates from different hosts in Turkey, such as PCR-RFLP of the ITS-1 region of the rRNA gene and sequencing of CO1 (15); comparative sequencing of the CO1 and/or ND1 genes (12)(13)(14); specific PCR analysis of the 12S rRNA gene and sequencing of CO1 (16,18); and specific PCR analysis of the 12S rRNA gene. Another study performed SSCP analysis of the CO1 gene and sequencing of CO1 (17).…”

Section: Discussionmentioning

confidence: 99%

“…According to these reports, G1 was the most prevalent strain among human and animal isolates. The G1 genotype has been demonstrated in humans, sheep, goats, cattle, camels, water buffaloes and dogs (12)(13)(14)(15)(16); the G3 genotype has been demonstrated in humans, sheep and cattle (12,13); and the G7 genotype has been demonstrated in humans and sheep (13), whereas G6 has only been identified in humans (18).…”

Section: Discussionmentioning

confidence: 99%

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Molecular Characterization of Human and Animal Isolates of Echinococcus granulosus in the Thrace Region, Turkey

Eryıldız¹,

Şakru²

2012

Balkan Med J

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“…The prevalence of CE in livestock varied from 3.5% to 58.6%, depending on different geographical locations and specimens examined (Altintas, 2008). CE was more prevalent in sheep (46.4%) in Van (Oguz and Deger, 2013) than in cattle (33.9%) in Erzurum , goats (22.1%) in Burdur (Umur, 2003) or buffaloes (10.2%) in the central Black Sea Region (Beyhan andUmur, 2011). p1470 In Iran, canine echinococcosis has been reported from across the country.…”

Section: U N C O R R E C T E D P R O O Fmentioning

confidence: 99%