Molecular Characterization and Phylogenetic Analysis of MADS-Box Gene VroAGL11 Associated with Stenospermocarpic Seedlessness in Muscadine Grapes

Rahman, Md. Ashiqur; Balasubramani, Subramani Paranthaman; Basha, Sheikh M.

doi:10.3390/genes12020232

Cited by 5 publications

(5 citation statements)

References 32 publications

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“…A weak VvAGL11 gene signal could be observed in the ovule primordia in the 4-5 cm inflorescences at the stage of ovule primordia formation through in situ hybridization [31]. Reduced expression of VvAGL11 is responsible for stenospermocarpic seedlessness in bunch grapes [32]. The VroAGL11 gene controlled the seed morphogenesis after ovule formation in 'Muscadine' grapes by analyzed its divergence from other plants of molecular level [32].…”

Section: Vvag Vvcrc and Vvagl11 Participate In Grape Ovary Developmentmentioning

confidence: 99%

“…Reduced expression of VvAGL11 is responsible for stenospermocarpic seedlessness in bunch grapes [32]. The VroAGL11 gene controlled the seed morphogenesis after ovule formation in 'Muscadine' grapes by analyzed its divergence from other plants of molecular level [32]. VvAGL11 transcripts exhibited a high accumulation in seeds after 2 and 4 weeks of development in 'Chardonnay' using in situ hybridization [30].…”

Section: Vvag Vvcrc and Vvagl11 Participate In Grape Ovary Developmentmentioning

confidence: 99%

“…On the formation process of ovule primordia of 'Xiangfei' grape, it was found that VvAG2 and VvAGL11 were involved in the formation of grape ovules [31]. In 'Muscadine' grape, VroAGL11 was found that the gene was most expressed in the berries with the highest seed content (weight) and almost not in seedless berries, indicating that VroAGL11 gene played an important role in controlling grape seed morphogenesis [32].…”

Section: Introductionmentioning

confidence: 99%

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VvAGAMOUS Affect Development of Four Different Grape Species Ovary

Zhang¹,

Zhang²,

Zhao³

et al. 2023

Phyton

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Grape pistil has an important influence on fruit size and quality. However, there were few studies on grape ovary, and the development process of the ovary is still unclear. Therefore, in this paper, four different grape varieties with different lengths of small inflorescences, namely 'Musct Hambourg' grape (Vitis vinifera), 'Concord' grape (Vitis labrusca), 'ShanPuTao' grape (Vitis amurensis) and 'GongNiang2Hao' grape (Vitis amurensis × Vitis vinifera) were used as test materials. Four varieties ovary were significant differences by means of stereomicroscope, paraffin section. The expression of ovary determining gene VvAGAMOUS (VvAG) and its development related genes VvCRABS CLAW (VvCRC) and VvAGAMOUS-LIKE 11 (VvAGL11) with similar functions during the development of different grape varieties were preliminarily explored using fluorescence quantitative test. The relationship between VvAG and VvCRC, VvAG and VvAGL11 were analyzed using Y1H assay. Our results showed that there were obvious abdominal sutures on the surface of expect for 'Musct Hambourg' grape, and existing poly carpels. The ovary development of 'ShanPuTao' and 'GongNiang2Hao' grape was completed when the inflorescence length was less than 1 cm, while the 'Concord' and 'Musct Hambourg' grape were fully developed when the length of inflorescence was 3-4 and 4-5 cm, respectively. VvAG and VvCRC began to express in large quantities after the formation of stamen primordia, while VvAGL11 during the forming of ovule primordia. Therefore, VvAG and VvCRC mainly regulated the development of stamens and carpels and also promote the development of ovules, while VvAGL11 major regulated the development of ovules. The promoters of VvCRC and VvAGL11 were bound by VvAG. This study provides an important theoretical basis for further research on the molecular mechanism of grape ovary development.

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Section: Vvag Vvcrc and Vvagl11 Participate In Grape Ovary Developmentmentioning

confidence: 99%

Section: Vvag Vvcrc and Vvagl11 Participate In Grape Ovary Developmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

VvAGAMOUS Affect Development of Four Different Grape Species Ovary

Zhang¹,

Zhang²,

Zhao³

et al. 2023

Phyton

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show abstract

“…In grapevines, VviAGL11 promotes lignification of the endosperm, a process that is a prerequisite for endosperm growth and embryo development; and disrupting VviAGL11 expression by RNAi in seeded grapevine varieties results in a significant reduction in both seed size and number [ 28 , 29 ]. In oil palm, mutations in the SHELL gene (the STK homolog), which controls ovule characteristics and seed development, causes the loss of the encapsulated cuticle of the seed kernels [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Analysis of MADS-Box Gene Family Reveals CjSTK as a Key Regulator of Seed Abortion in Camellia japonica

Yu,

Chu,

et al. 2024

IJMS

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The plant MADS-box transcription factor family is a major regulator of plant flower development and reproduction, and the AGAMOUS-LIKE11/SEEDSTICK (AGL11/STK) subfamily plays conserved functions in the seed development of flowering plants. Camellia japonica is a world-famous ornamental flower, and its seed kernels are rich in highly valuable fatty acids. Seed abortion has been found to be common in C. japonica, but little is known about how it is regulated during seed development. In this study, we performed a genome-wide analysis of the MADS-box gene the in C. japonica genome and identified 126 MADS-box genes. Through gene expression profiling in various tissue types, we revealed the C/D-class MADS-box genes were preferentially expressed in seed-related tissues. We identified the AGL11/STK-like gene, CjSTK, and showed that it contained a typical STK motif and exclusively expressed during seed development. We found a significant increase in the CjSTK expression level in aborted seeds compared with normally developing seeds. Furthermore, overexpression of CjSTK in Arabidopsis thaliana caused shorter pods and smaller seeds. Taken together, we concluded that the fine regulation of the CjSTK expression at different stages of seed development is critical for ovule formation and seed abortion in C. japonica. The present study provides evidence revealing the regulation of seed development in Camellia.

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“…VvAGL11 has been proposed as the major candidate gene involved in Vitis vinifera seed morphogenesis, and its expression is responsible for the erroneous development of a highly essential integument layer [ 22 ]. Rahman et al characterize the molecular level of VroAGL11 in Vitis rotundifolia and analyze its divergence from other plants, suggesting that the VroAGL11 gene controls the seed morphogenesis after ovule formation in muscadine grapes [ 23 ]. The formation of the ovule is the premise of seed forming.…”

Section: Introductionmentioning

confidence: 99%

MADS-Box Protein Complex VvAG2, VvSEP3 and VvAGL11 Regulates the Formation of Ovules in Vitis vinifera L. cv. ‘Xiangfei’

Yan

Liu

et al. 2021

Genes

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The phenomenon of multi-carpel and multi-ovule exists in the grapevine cultivar ‘Xiangfei’, but the mechanism of ovule formation is seldom reported. In this study, we observed the ovule formation process by using ‘Xiangfei’ grapes. The role of the VvAG2 (VvAGAMOUS) gene in ovule formation was identified, and we explored the relationship between VvAG2, VvSEP3(VvMADS4) and VvAGL11(VvMADS5) proteins. The results showed that the ovule primordium appeared when the inflorescence length of ‘Xiangfei’ grapes were 4–5 cm long; the relative expression levels of VvAG2, VvAGL11 and VvSEP3 genes were higher during ovule formation, and the expression levels of VvAG2 gene was the highest. Transgenic tomato (Solanum lycopersicum) plants expressing VvAG2 produced higher numbers of ovules and carpels than the wild type. Moreover, yeast two-hybrid and yeast three-hybrid experiments demonstrated that VvSEP3 acts as a bridge and interacts with VvAG2 and VvAGL11 proteins, respectively. Meanwhile, a homodimer can be formed between VvSEP3 and VvSEP3, but there was no interaction between VvAG2 and VvAGL11. These findings suggest that the VvAG2 gene is involved in the formation of ovules, and VvAG2/VvSEP3 together with VvAGL11/VvSEP3 can form a tetrameric complex. In summary, our data showed that VvAG2 along with VvSEP3 and VvAGL11 jointly regulate the ovule formation of ‘Xiangfei’ grapes.

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Molecular Characterization and Phylogenetic Analysis of MADS-Box Gene VroAGL11 Associated with Stenospermocarpic Seedlessness in Muscadine Grapes

Cited by 5 publications

References 32 publications

VvAGAMOUS Affect Development of Four Different Grape Species Ovary

VvAGAMOUS Affect Development of Four Different Grape Species Ovary

Genome-Wide Analysis of MADS-Box Gene Family Reveals CjSTK as a Key Regulator of Seed Abortion in Camellia japonica

MADS-Box Protein Complex VvAG2, VvSEP3 and VvAGL11 Regulates the Formation of Ovules in Vitis vinifera L. cv. ‘Xiangfei’

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