MADS-Box Protein Complex VvAG2, VvSEP3 and VvAGL11 Regulates the Formation of Ovules in Vitis vinifera L. cv. ‘Xiangfei’

Yan, Wang; Liu, Zhenhua; Wu, Jiang; Luan, Hong; Liang, Jinjun; Ren, Yangmei; Guan, Pingyin; Hu, Jing

doi:10.3390/genes12050647

Cited by 9 publications

(14 citation statements)

References 50 publications

(60 reference statements)

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“…Moreover, we evaluated the potential interaction between VvMADS39 and VvAG using bimolecular fluorescence complementation (BiFC). The interaction between VvSEP3 and VvAGL11, which has been previously tested (Wang et al., 2021), was used as the positive control. Moreover, two additional groups (VvMADS39–VvAGL11 and VvAG–VvAGL11) were used as the negative controls, because they did not show any interaction in yeast.…”

Section: Resultsmentioning

confidence: 99%

“…SEP3 is known to participate in several protein complexes as a bridge (Favaro et al., 2003). For instance, it can mediate complexation with different genes, such as STK , AG , SHP1/2 , and BELL , to regulate ovule development (Brambilla et al., 2007; Favaro et al., 2003; Wang et al., 2021). Although SEP3 is sufficient for normal ovule development, the fact that other genes in the SEP subfamily have been preserved during evolution suggests that they may have different functions and not be completely redundant (Mandel & Yanofsky, 1998; Savidge et al., 1995).…”

Section: Resultsmentioning

confidence: 99%

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Role of grapevine SEPALLATA‐related MADS‐box gene VvMADS39 in flower and ovule development

et al. 2022

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Seedlessness is one of the most important breeding goals for table grapes; thus, understanding the molecular genetic regulation of seed development and abortion is critical for the development of seedless cultivars.In the present study, we characterized VvMADS39, a class E MADS-box gene of grapevine (Vitis vinifera) orthologous to Arabidopsis SEP2. Heterologous overexpression of VvMADS39 in tomato reduced the fruit and seed size and seed number. Targeted mutagenesis of the homologous SlMADS39 in tomato induced various floral and fruit defects. It could reasonable to suppose that active VvMADS39 expression in "Thompson Seedless" may restrict cellular expansion, resulting in the development of smaller fruits and seeds, VvMADS39 may play a role in the regulation of ovule development in grapevine and contributes to seedless fruit formation. In contrast, VvMADS39 suppression in "Red Globe" was associated with enhanced histone H3 lysine 27 trimethylation in the promoter region of VvMADS39, allowing normal ovule and fruit development; Meanwhile, VvMADS39 interacts with VvAGAMOUS, and the activity of the VvMADS39-VvAGA-MOUS dimer to induce integument development requires the activation and maintenance of VvINO expression. The synergistic cooperation between VvMADS39 and related proteins plays an important role in maintaining floral meristem characteristics, and fruit and ovule development.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Role of grapevine SEPALLATA‐related MADS‐box gene VvMADS39 in flower and ovule development

et al. 2022

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“…6d ). In our model, we speculate on a tetrameric organization of the complex involving only VviAGL11, but it should be taken into account the possibility of VviAGL11 interacting with other co-factors as previously proposed [ 11 , 24 ]. As shown in Figure 6 , in this latter hypothesis the combination of the WT AGL11 with the HYB or MUT protein may affect its ability to organize higher order complex and to exert its function on target genes.…”

Section: Discussionmentioning

confidence: 79%

“…VviAGL11 is a MIKC c -type gene, classified as a class D MADS-box gene, whose ability to establish higher-order complexes has already been demonstrated often [ 22 ], mediated by the presence of the SEP clade proteins [ 23 ]. For example, it has been reported that the grape MADS-box family transcription factor complexes VviAG2/VviSEP3 and VviAGL11/VviSEP3 form tetramers that may be involved in the formation of ovules before flowering and bloom [ 24 ]. On the other hand, we focused on the role of VviAGL11 on stenospermocarpy, studying a different developmental phase and this will likely involve different interactions.…”

Section: Discussionmentioning

confidence: 99%

VviAGL11 self-regulates and targets hormone- and secondary metabolism-related genes during seed development

Amato

Cardone

Ocarez

et al. 2022

Horticulture Research

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VviAGL11, the Arabidopsis SEEDSTICK homolog, has been proposed to have a causative role in grapevine stenospermocarpy. An association between a mutation in the coding sequence (CDS) and the seedless phenotype was reported, however, no working mechanisms have been demonstrated yet. We performed a deep investigation of the full VviAGL11 gene sequence in a collection of grapevine varieties belonging to several seedlessness classes that revealed three different promoter-CDS combinations. By investigating the expression of the three VviAGL11 alleles, and by evaluating their ability to activate the promoter region, we observed that VviAGL11 self-activates in a specific promoter-CDS combination manner. Furthermore, by transcriptomic analyses on ovule and developing seeds in seeded and seedless varieties and co-expression approaches, candidate VviAGL11 targets were identified and further validated through luciferase assay and in situ hybridization. We demonstrated that VviAGL11 Wild Type CDS activates Methyl jasmonate esterase and Indole-3-acetate beta-glucosyltransferase, both involved in hormone signaling and Isoflavone reductase, involved in secondary metabolism. The dominant-negative effect of the mutated CDS was also functionally ectopically validated in target induction. VviAGL11 was shown to co-localize with its targets in the outer seed coat integument, supporting its direct involvement in seed development, possibly by orchestrating the crosstalk among MeJA, auxin, and isoflavonoids synthesis. In conclusion, the VviAGL11 expression level depends on the promoter-CDS allelic combination, and this will likely affect its ability to activate important triggers of the seed coat development. The dominant-negative effect of the mutated VviAGL11 CDS on the target genes activation was molecularly validated. A new regulatory mechanism correlating VviAGL11 haplotype assortment and seedlessness class in grapevine is proposed.

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“…In the process of studying the molecular mechanism of controlling seedless grapes found VvAGL11 transcription was essential for seed morphogenesis in grapevine during berry development [30]. On the formation process of ovule primordia of 'Xiangfei' grape, it was found that VvAG2 and VvAGL11 were involved in the formation of grape ovules [31]. In 'Muscadine' grape, VroAGL11 was found that the gene was most expressed in the berries with the highest seed content (weight) and almost not in seedless berries, indicating that VroAGL11 gene played an important role in controlling grape seed morphogenesis [32].…”

Section: Introductionmentioning

confidence: 99%

VvAGAMOUS Affect Development of Four Different Grape Species Ovary

Zhang¹,

Zhang²,

Zhao³

et al. 2023

Phyton

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Grape pistil has an important influence on fruit size and quality. However, there were few studies on grape ovary, and the development process of the ovary is still unclear. Therefore, in this paper, four different grape varieties with different lengths of small inflorescences, namely 'Musct Hambourg' grape (Vitis vinifera), 'Concord' grape (Vitis labrusca), 'ShanPuTao' grape (Vitis amurensis) and 'GongNiang2Hao' grape (Vitis amurensis × Vitis vinifera) were used as test materials. Four varieties ovary were significant differences by means of stereomicroscope, paraffin section. The expression of ovary determining gene VvAGAMOUS (VvAG) and its development related genes VvCRABS CLAW (VvCRC) and VvAGAMOUS-LIKE 11 (VvAGL11) with similar functions during the development of different grape varieties were preliminarily explored using fluorescence quantitative test. The relationship between VvAG and VvCRC, VvAG and VvAGL11 were analyzed using Y1H assay. Our results showed that there were obvious abdominal sutures on the surface of expect for 'Musct Hambourg' grape, and existing poly carpels. The ovary development of 'ShanPuTao' and 'GongNiang2Hao' grape was completed when the inflorescence length was less than 1 cm, while the 'Concord' and 'Musct Hambourg' grape were fully developed when the length of inflorescence was 3-4 and 4-5 cm, respectively. VvAG and VvCRC began to express in large quantities after the formation of stamen primordia, while VvAGL11 during the forming of ovule primordia. Therefore, VvAG and VvCRC mainly regulated the development of stamens and carpels and also promote the development of ovules, while VvAGL11 major regulated the development of ovules. The promoters of VvCRC and VvAGL11 were bound by VvAG. This study provides an important theoretical basis for further research on the molecular mechanism of grape ovary development.

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MADS-Box Protein Complex VvAG2, VvSEP3 and VvAGL11 Regulates the Formation of Ovules in Vitis vinifera L. cv. ‘Xiangfei’

Cited by 9 publications

References 50 publications

Role of grapevine SEPALLATA‐related MADS‐box gene VvMADS39 in flower and ovule development

Role of grapevine SEPALLATA‐related MADS‐box gene VvMADS39 in flower and ovule development

VviAGL11 self-regulates and targets hormone- and secondary metabolism-related genes during seed development

VvAGAMOUS Affect Development of Four Different Grape Species Ovary

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