Molecular characterization and pathogenicity of a virulent Acinetobacter baumannii associated with mortality of farmed Indian Major Carp Labeo rohita (Hamilton 1822)

Behera, Bijay Kumar; Paria, Prasenjit; Das, A. K.; Bhowmick, S.; Sahoo, Amiya Kumar; Das, Basanta Kumar

doi:10.1016/j.aquaculture.2017.01.018

Cited by 48 publications

(22 citation statements)

References 14 publications

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“…High prevalence of A. baumannii in different types of milk samples was also previously reported by Vaz-Moreira et al (2011) [33], Gurung et al [34], Saadet al [35], and Ramos and Nascimento [36].A. baumannii and other Acinetobacter species were rarely detected in seafood samples [37][38][39][40]. In keeping with this, this pathogen's role in seafood samples is potentially unknown, and further research is required to understand this fact.…”

Section: Discussionsupporting

confidence: 70%

The First Report of the Antimicrobial Resistance of Acinetobacter baumannii Isolates from Seafood Samples

et al. 2021

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A cinetobacterbaumannii is a newly-launched bacterium responsible for rare cases of diarrhea and foodborne diseases. This survey was performed to study the prevalence and antimicrobial resistance of A. baumannii isolates recovered from fish, shrimp, and lobster. Five-hundred seafood samples were collected and cultured for isolation of A. baumannii. A. baumannii isolates were identified by biochemical tests and further confirmed by the PCR. The antimicrobial resistance of isolates was studied using disk diffusion test. Twenty-eight out of 500 (5.6%) seafood samples were contaminated with A. baumannii. Fish had the highest prevalence of A. baumannii (10%), while lobster had the lowest (2.5%). A. baumannii isolates harbored the highest incidence of resistance toward tetracycline (75%), gentamicin (71.4%), ciprofloxacin (64.2%), trimethoprim (57.1%), streptomycin (53.5%), and erythromycin (50%) antimicrobial agents. The lowest incidence of resistance was obtained against chloramphenicol (3.5%) and imipenem (7.1%) antimicrobial agents. This is the first report of isolation and identification and antimicrobial resistance pattern of A. baumannii in seafood samples globally. The role of seafood samples as reservoirs of antimicrobial-resistant A. baumannii was determined. Complete cooking of seafood and prevention from their consumption in raw form can diminish the risk of A. baumannii foodborne diseases.

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Section: Discussionsupporting

confidence: 70%

The First Report of the Antimicrobial Resistance of Acinetobacter baumannii Isolates from Seafood Samples

et al. 2021

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“…In this study, a large number of pathogens identified in fish, such as bacterium 2013Ark19i (Bacteria_norank) (25.39%), bacterium 2013Arg42i (Bacteria no_rank) (15.27%), and Acinetobacter baumannii (9.79%) (Xia et al, 2008;Seth-Smith et al, 2016;Behera et al, 2017), existed in the stomach of yellowfin sea bream, and there were also many pathogenic microorganisms that affected fish health, such as Flavobacterium, Aeromonas, and Pseudomonas (Chen et al, 2018). However, the pathogenic bacteria reported in the intestinal tract of yellowfin sea bream, such as Streptococcus iniae, and Vibrio harveyi (Zhou et al, 1996;Wang et al, 2018), were not found in the stomach of yellowfin sea bream.…”

Section: Composition and Functional Structures Of The Stomach Microbiome Of Yellowfin Sea Breammentioning

confidence: 88%

Is Metagenomic Analysis an Effective Way to Analyze Fish Feeding Habits? A Case of the Yellowfin Sea Bream Acanthopagrus latus (Houttuyn) in Daya Bay

et al. 2021

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Yellowfin seabream, Acanthopagrus latus, is one of the most important species in terms of stock enhancement in China. However, using metagenomic techniques to explore the feeding habits and stomach microbiome of yellowfin seabream is still rare. The objective of this work was to study the feeding habits and stomach microbiome of yellowfin sea bream from Daya Bay through metagenomic analysis of different weight classes (≤50, 50–100, and >100 g). Whole-metagenome shotgun sequencing and morphological observation were used to investigate the stomach contents. The dietary composition and the community composition of the stomach microbiome of A. latus were examined. In this study, 153 species were detected in the eukaryotic composition of the stomach contents of yellowfin sea bream. At the species level, Mytilus edulis was the only species identified by both metagenomic analysis and morphological observation. The proportion of fish and bivalves was over 98%, but the diet changed little with body size. Larimichthys crocea, Scophthalmus maximus, and Seriola dumerili were the most abundant species among all samples. In total, 285 species were identified in the stomach microbiome of yellowfin sea bream. Bacterium 2013Ark19i, bacterium 2013Arg42i and Acinetobacter baumannii, first reported in the stomach contents of yellowfin sea bream, were the most abundant species of the stomach microbiomes. There was no difference in the biodiversity of the stomach microbiomes among the different body sizes. Overall, the composition of the yellowfin sea bream diet mainly consists of fish and bivalves. The use of metagenomics techniques is a promising approach for assessing the feeding habits of yellowfin sea bream. The results derived from this study can provide important information for evaluating the feeding ecology of yellowfin sea bream in Daya Bay.

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“…One of the most promising isolates of the present culture collection is Acinetobacter haemolyticus , revealing strong antagonism towards T. maritimum , an interesting profile of essential fatty acids and the ability to metabolize saponin (Table 2 ). Interestingly, beneficial Acinetobacter species have been used as probiotics in shrimp aquaculture [ 63 ] but to our knowledge no Acinetobacter species have been evaluated as probiotic supplement in fish farming, probably due to increasing reports on opportunistic fish pathogens within this genus [ 64 – 67 ]. However, our isolate assigned to A. haemolyticus seems to be taxonomically not related to pathogenic Acinetobacter species.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of native probiotics for fish farming

et al. 2018

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BackgroundInnovations in fish nutrition act as drivers for the sustainable development of the rapidly expanding aquaculture sector. Probiotic dietary supplements are able to improve health and nutrition of livestock, but respective bacteria have mainly been isolated from terrestrial, warm-blooded hosts, limiting an efficient application in fish. Native probiotics adapted to the gastrointestinal tract of the respective fish species will establish within the original host more efficiently.ResultsHere, 248 autochthonous isolates were cultured from the digestive system of three temperate flatfish species. Upon 16S rRNA gene sequencing of 195 isolates, 89.7% (n = 175) Gram-negatives belonging to the Alpha- (1.0%), Beta- (4.1%) and Gammaproteobacteria (84.6%) were identified. Candidate probiotics were further characterized using in vitro assays addressing 1) inhibition of pathogens, 2) degradation of plant derived anti-nutrient (saponin) and 3) the content of essential fatty acids (FA) and their precursors. Twelve isolates revealed an inhibition towards the common fish pathogen Tenacibaculum maritimum, seven were able to metabolize saponin as sole carbon and energy source and two isolates 012 Psychrobacter sp. and 047 Paracoccus sp. revealed remarkably high contents of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). Furthermore, a rapid and cost-effective method to coat feed pellets revealed high viability of the supplemented probiotics over 54 d of storage at 4°C.ConclusionsHere, a strategy for the isolation and characterization of native probiotic candidates is presented that can easily be adapted to other farmed fish species. The simple coating procedure assures viability of probiotics and can thus be applied for the evaluation of probiotic candidates in the future.

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Molecular characterization and pathogenicity of a virulent Acinetobacter baumannii associated with mortality of farmed Indian Major Carp Labeo rohita (Hamilton 1822)

Cited by 48 publications

References 14 publications

The First Report of the Antimicrobial Resistance of Acinetobacter baumannii Isolates from Seafood Samples

The First Report of the Antimicrobial Resistance of Acinetobacter baumannii Isolates from Seafood Samples

Is Metagenomic Analysis an Effective Way to Analyze Fish Feeding Habits? A Case of the Yellowfin Sea Bream Acanthopagrus latus (Houttuyn) in Daya Bay

Isolation and characterization of native probiotics for fish farming

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