Molecular characterization and mRNA expression during metal exposure and thermal stress of copper/zinc- and manganese-superoxide dismutases in disk abalone, Haliotis discus discus

Kim, Keun-Yong; Lee, Sang Yoon; Cho, Young Sun; Bang, In Chul; Kim, Ki Hong; Kim, Dong Soo; Nam, Yoon Kwon

doi:10.1016/j.fsi.2007.04.010

Cited by 115 publications

(76 citation statements)

References 38 publications

(26 reference statements)

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“…MnSOD and Cu/Zn SOD are found in both prokaryotes and eukaryotes, FeSOD is found in prokaryotes and plants [1], and NiSOD has recently been purified from several aerobic soil bacteria of Streptomyces [2]. SOD is one of the sensitive biomarker to indicate organisms being under stress [3,4].…”

Section: Introductionmentioning

confidence: 99%

“…Both the transcription and enzyme activity of Cu/Zn SOD are sensitive to stresses such as exposure to heavy metals or biocides, like tributyltin, heat shock, and anoxia [3,4,14,15]. Cu/Zn SOD genes have been cloned from several aquatic species including frog, Xenopus laevis [16], grouper, Epinephelus malbaricus [17], Pacific oyster, Crassostrea gigas [18] and the abalones Haliotis discus discus [4].…”

Section: Introductionmentioning

confidence: 99%

“…Cu/Zn SOD genes have been cloned from several aquatic species including frog, Xenopus laevis [16], grouper, Epinephelus malbaricus [17], Pacific oyster, Crassostrea gigas [18] and the abalones Haliotis discus discus [4]. So far, the Cu/Zn SOD from pearl oysters has not been elucidated.…”

Section: Introductionmentioning

confidence: 99%

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Molecular cloning, characterization and expression analysis of cytoplasmic Cu/Zn-superoxid dismutase (SOD) from pearl oyster Pinctada fucata

Anju

Jeswin

Thomas

et al. 2013

Fish & Shellfish Immunology

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a b s t r a c tBecause of its capacity to rapidly convert superoxide to hydrogen peroxide, superoxide dismutase (SOD) is crucial in both intracellular signalling and regulation of oxidative stress. In this paper we report the cloning of a Cu/Zn SOD (designated as pfSOD) from the pearl oyster (Pinctada fucata) using rapid amplification of cDNA ends (RACE) PCR. The full-length cDNA of this Cu/Zn SOD contains an open reading frame (ORF) of 471 bp coding for 156 amino acids. No signal peptide was identified at the N-terminal amino acid sequence of Cu/Zn SOD indicating that this pfSOD encodes a cytoplasmic Cu/Zn SOD. This is supported by the presence of conserved amino acids required for binding copper and zinc. Semi-quantitative analysis in adult tissues showed that the pfSOD mRNA was abundantly expressed in haemocytes and gill and scarcely expressed in other tissues tested. After challenge with lipopolysaccharide (LPS), expression of pfSOD mRNA in haemocytes was increased, reaching the highest level at 8 h, then dropping to basal levels at 36 h. These results suggest that Cu/Zn SOD might be used as a bioindicator of the aquatic environmental pollution and cellular stress in pearl oyster.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular cloning, characterization and expression analysis of cytoplasmic Cu/Zn-superoxid dismutase (SOD) from pearl oyster Pinctada fucata

Anju

Jeswin

Thomas

et al. 2013

Fish & Shellfish Immunology

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“…Generation of reactive oxygen species (ROS) and reactive oxygen intermediates (ROI) is an unavoidable consequence in most aerobic organisms [1]. ROS, on the one hand, can stimulate signal transduction pathways like NF-kB or MAPK [2e4], and mediate various responses such as cell growth and apoptosis [5,6], or be responsible for killing invading microorganisms [7].…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of an intracellular Cu, Zn-superoxide dismutase (icCu/Zn-SOD) gene from clam Venerupis philippinarum

Li¹,

Sun²,

Chen³

et al. 2010

Fish & Shellfish Immunology

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“…It can increase phagocytic cell activity and immune function and protect the cell from ROS poisoning (Kim et al. 2007). The expression pattern of Cu/Zn‐SOD was JJ (1.057) > VV (1.036) > TT (0.36).…”

Section: Discussionmentioning

confidence: 99%

Protein changes in abalone foot muscle from three geographical populations of Haliotis diversicolor based on proteomic approach

Miao

et al. 2016

Ecology and Evolution

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Using two‐dimensional gel electrophoresis, the foot muscle proteome of three geographical populations of Haliotis diversicolor were examined, with a total of 922 ± 21 protein spots detected in the Japanese population (JJ), 904 ± 25.6 in the Taiwanese population (TT), and 936 ± 16.2 in the Vietnamese population (VV). Of these, 254 spots showed differential expression and 85 protein spots percentage volumes varied more than twofold. Both “genotype” and “spot” analysis of variance approaches significantly showed differences among the three populations. Hierarchical clustering analysis showed that TT and VV clustered together followed by clustering with JJ, which is consistent with their geographical location. Following matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry, 30 differentially expressed proteins involved in major biological processes including energy production and storage and stress response were identified. Of these proteins, proteins pertaining to muscle contraction and muscle protein regulation showed highest expression levels in VV samples. Proteins involved in energy production and storage, including ATP synthase beta subunit, fructose‐1,6‐bisphosphate aldolase, arginine kinase, enolase, triosephosphate isomerase, and tauropine dehydrogenase, showed diverse expression patterns among the three populations. For stress‐responsive proteins, the expression of heat shock protein 70 was JJ > VV > TT. The expression pattern of Cu/Zn‐superoxide dismutase was JJ > VV > TT. Overall, these results may aid in the detection of new differentially expressed proteins within three different abalone populations.

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Molecular characterization and mRNA expression during metal exposure and thermal stress of copper/zinc- and manganese-superoxide dismutases in disk abalone, Haliotis discus discus

Cited by 115 publications

References 38 publications

Molecular cloning, characterization and expression analysis of cytoplasmic Cu/Zn-superoxid dismutase (SOD) from pearl oyster Pinctada fucata

Molecular cloning, characterization and expression analysis of cytoplasmic Cu/Zn-superoxid dismutase (SOD) from pearl oyster Pinctada fucata

Identification and characterization of an intracellular Cu, Zn-superoxide dismutase (icCu/Zn-SOD) gene from clam Venerupis philippinarum

Protein changes in abalone foot muscle from three geographical populations of Haliotis diversicolor based on proteomic approach

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