Molecular characterization and morphological clustering of exotic early maturing rice (Oryza sativa L.) lines

Abstract

Characterization and variability analysis is important for the improvement of crop plant. This study aimed to evaluate the morphological and molecular variation of exotic early maturing rice (Oryza sativa L.) lines. A total of 32 exotic rice lines collected from different locations were genotyped and clustered using selected SSR markers. Based on morphological dendrogram, the lines were grouped into three clusters viz.I, II and III. Cluster I, cluster II and cluster III had 12, 11, 9 lines respectively. The results showed that the varieties were closely related belonging to the same cluster. DNA Markers namely Simple Sequence Repeats (SSR) is a useful tool for assessing genetic variations and resolving cultivar identities. Positive correlations were found between gene diversity, number of allele, the allele size range and the maximum number of repeats. Among the primers used RM147 identified more number of alleles and average PIC was 0.88. The UPGMA dendrogram based on Nei's (1972) genetic distance grouped the 32 rice lines into three major clusters. This result indicates that the line which formed grouped together, they are less diverse. A significant level of polymorphism based on morphological and molecular levels was observed. Being grouped into three clusters C1-4-11-7P-2P-1P and IR 79201-49-1-1-1 could be utilized as potential parents for the improvement of yield in early maturing rice lines.

Estimation of Genetic Variability, Correlation and Path analysis in short duration Rice Genotypes of Manipur

“…number of spikelets per panicle (0.821, 6.631), day to maturity (0.889, 1.542) and panicle length (0.052, 0.992) resulted in highly significant association with grain yield per plant. Similar report was reported by and Mehetre et al 2003Sarker et al 2014 Sarma Sharma Table 2: Phenotypic and genotypic coefficient of variation, heritability and genetic advance (as % of mean) among Short duration genotypes for yield and yield attributing traits Table 3: Estimates of phenotypic and genotypic correlation coefficient for yield and yield attributing traits (*: Significant at 5% level of significance, **: Signficant at 1% level of significance) ( ). At the both phenotypic and genotypic level, days to maturity had positive indirect effect via, number of spikelets per panicle (0.436, 2.793), while it had positive indirect effect via plant height (0.817) at the phenotypic level only.…”

Genetic Diversity and Population Structure of Basmati Rice (Oryza sativa L.) Germplasm Collected from North Western Himalayas Using Trait Linked SSR Markers

“…Identification of diverse genotypes using molecular markers is advantageous over the conventional approach [ 39 ]. SSRs molecular markers have been widely applied in the genetic diversity analysis, genotypic identification and population structure estimation in several rice genetic studies, including basmati rice [ 1 , 4 – 11 , 40 – 45 ]. In the present study, 38 out of 40 markers were polymorphic and produced unique allelic profiles for the 141 basmati rice genotypes.…”

Genetic divergence and cluster analysis for bulb yield and related traits in garlic (Allium sativum) accessions at Dorze, southern Ethiopia

“…The higher distance between groups indicated greater genetic divergence between the genotypes of these groups (Dejen et al., 2021; Yadav, 2018). The selection of parents for hybridization should be made from two clusters with larger distances between the clusters to achieve maximum variability in the separation of the generations (Sarker et al., 2013; Viana et al., 2015; Hoogerheide et al., 2017). However, the smallest intercluster distance was observed between Clusters II and III ( D 2 = 2.96) followed by Clusters I and VII ( D 2 = 2.93).…”