Molecular characterization and localization of the first tyramine receptor of the American cockroach (Periplaneta americana)

Rotte, Cathleen; Krach, Christian; Balfanz, Sabine; Baumann, Arnd; Walz, Bernd; Blenau, Wolfgang

doi:10.1016/j.neuroscience.2009.05.066

Cited by 51 publications

(83 citation statements)

References 60 publications

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“…Recent reviews have hypothesized, based on sequence data, that this receptor is a TAR1 (this class was previously known as octopamine/tyramine or tyramine receptor), which would normally signal through the Ga i pathway (Ohta and Ozoe, 2014;Verlinden et al, 2010;Farooqui, 2012). Several TAR1 receptors have been identified and second messenger pathways analyzed from Apis mellifera, AJ245824 (Blenau et al, 2000), Bombyx mori, AB162828 (Ohta et al, 2003), Chilo Suppressalis, JQ416145 (Wu et al, 2013), Drosophila melanogaster, X54794 (Saudou et al, 1990), Heliothis virescens, X95606 (von Nickisch-Rosenegk et al, 1996), Locusta migratoria, X69520 (Vanden Broeck et al, 1995) and Periplaneta americana, AM990461 (Rotte et al, 2009). …”

Section: Discussionmentioning

confidence: 99%

“…Yohimbine is a known a 2 -adrenergic antagonist, and has previously been shown to be effective at blocking the inhibitory effect of tyramine on forskolinstimulated cAMP in heterologous expression systems (Ohta et al, 2003;Rotte et al, 2009;Wu et al, 2013). Rotte et al (2009) found that yohimbine produced the strongest antagonistic effect, followed by chlorpromazine then cyproheptadine. However, we find that yohimbine and cyproheptadine produce the strongest antagonistic effect, at the three tested concentrations, followed by mianserin (significant antagonism at 1 mM and 10 mM), then followed by phentolamine (significant at 10 mM).…”

Section: Discussionmentioning

confidence: 99%

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Pharmacological characterization of a tyramine receptor from the southern cattle tick, Rhipicephalus (Boophilus) microplus

Gross

Temeyer

Day

et al. 2015

Insect Biochemistry and Molecular Biology

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The southern cattle tick (Rhipicephalus (Boophilus) microplus) is a hematophagous external parasite that vectors the causative agents of bovine babesiosis or cattle tick fever, Babesia bovis and B. bigemina, and anaplasmosis, Anaplasma marginale. The southern cattle tick is a threat to the livestock industry in many locations throughout the world. Control methods include the use of chemical acaricides including amitraz, a formamidine insecticide, which is proposed to activate octopamine receptors. Previous studies have identified a putative octopamine receptor from the southern cattle tick in Australia and the Americas. Furthermore, this putative octopamine receptor could play a role in acaricide resistance to amitraz. Recently, sequence data indicated that this putative octopamine receptor is probably a type-1 tyramine receptor (TAR1). In this study, the putative TAR1 was heterologously expressed in Chinese hamster ovary (CHO-K1) cells, and the expressed receptor resulted in a 39-fold higher potency for tyramine compared to octopamine. Furthermore, the expressed receptor was strongly antagonized by yohimbine and cyproheptadine, and mildly antagonized by mianserin and phentolamine. Tolazoline and naphazoline had agonistic or modulatory activity against the expressed receptor, as did the amitraz metabolite, BTS-27271; however, this was only observed in the presence of tyramine. The southern cattle tick's tyramine receptor may serve as a target for the development of anti-parasitic compounds, in addition to being a likely target of formamidine insecticides. RightsWorks produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted. a b s t r a c tThe southern cattle tick (Rhipicephalus (Boophilus) microplus) is a hematophagous external parasite that vectors the causative agents of bovine babesiosis or cattle tick fever, Babesia bovis and B. bigemina, and anaplasmosis, Anaplasma marginale. The southern cattle tick is a threat to the livestock industry in many locations throughout the world. Control methods include the use of chemical acaricides including amitraz, a formamidine insecticide, which is proposed to activate octopamine receptors. Previous studies have identified a putative octopamine receptor from the southern cattle tick in Australia and the Americas. Furthermore, this putative octopamine receptor could play a role in acaricide resistance to amitraz. Recently, sequence data indicated that this putative octopamine receptor is probably a type-1 tyramine receptor (TAR1). In this study, the putative TAR1 was heterologously expressed in Chinese hamster ovary (CHO-K1) cells, and the expressed receptor resulted in a 39-fold higher potency for tyramine compared to octopamine. Furthermore, the expressed receptor was strongly antagonized by yohimbine and cyproheptadine, and mildly antagonized by mianserin and phentolamine. Tolazoline and naphazoline had agonistic or modulatory activity against the expresse...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Pharmacological characterization of a tyramine receptor from the southern cattle tick, Rhipicephalus (Boophilus) microplus

Gross

Temeyer

Day

et al. 2015

Insect Biochemistry and Molecular Biology

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“…CsOA2B2 was stably expressed in HEK-293 cells, which have been used successfully in previous studies to examine the pharmacological properties of cloned insect GPCRs (Bischof and Enan, 2004;Han et al, 1998;Huang et al, 2010;Rotte et al, 2009 ) determined in CHO and HEK-293 cells, respectively. In agonist assays, naphazoline was less potent than octopamine but more potent than clonidine, which is consistent with that of the classical type-2A octopamine receptors (Evans, 1981).…”

Section: Discussionmentioning

confidence: 99%

“…The OctR class is subdivided into three subclasses and they each specifically increase [cAMP] i levels in response to octopamine. The TyrR class, which shows structural and pharmacological similarities with vertebrate  2 -adrenergic receptors, was preferentially activated by tyramine to induce a decrease in [cAMP] i (Blenau et al, 2000;Ohta et al, 2003;Rotte et al, 2009;Saudou et al, 1990). For this reason, researchers thought that this class of receptors represents a class of tyramine receptors (Lange, 2009).…”

Section: Introductionmentioning

confidence: 99%

Characterization of a β-adrenergic-like octopamine receptor from the rice stem borer (Chilo suppressalis)

Yao

Huang

et al. 2012

Journal of Experimental Biology

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SUMMARYOctopamine, the invertebrate counterpart of adrenaline and noradrenaline, plays a key role in regulation of many physiological and behavioral processes in insects. It modulates these functions through binding to specific octopamine receptors, which are typical rhodopsin-like G-protein coupled receptors. A cDNA encoding a seven-transmembrane receptor was cloned from the nerve cord of the rice stem borer, Chilo suppressalis, viz. CsOA2B2, which shares high sequence similarity to CG6989, a Drosophila -adrenergic-like octopamine receptor (DmOct2R). We generated an HEK-293 cell line that stably expresses CsOA2B2 in order to examine the functional and pharmacological properties of this receptor. Activation of CsOA2B2 by octopamine increased the production of cAMP in a dose-dependent manner (EC 50 2.33nmoll Supplementary material available online at

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“…Heterologous expression of this tyramine receptor indicated it couples to the inositol pathway when expressed with a Ga qi(5) -protein chimeria [15]. In addition, tyramine receptors have been identified in a variety of organisms including Drosophila melanogaster [2,4], Bombyx mori [20,33], Chilo suppressalis [54], Periplaneta americana [45] and Apis mellifera [3]. The objective of this study was to determine if essential oil terpenoids could interact with a tyramine receptor from the southern cattle tick.…”

Section: Introductionmentioning

confidence: 99%

Interaction of plant essential oil terpenoids with the southern cattle tick tyramine receptor: A potential biopesticide target

Gross

Temeyer

Day

et al. 2017

Chemico-Biological Interactions

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a b s t r a c tAn outbreak of the southern cattle tick, Rhipicephalus (Boophilus) microplus, (Canestrini), in the United States would have devastating consequences on the cattle industry. Tick populations have developed resistance to current acaricides, highlighting the need to identify new biochemical targets along with new chemistry. Furthermore, acaricide resistance could further hamper control of tick populations during an outbreak. Botanically-based compounds may provide a safe alternative for efficacious control of the southern cattle tick. We have developed a heterologous expression system that stably expresses the cattle tick's tyramine receptor with a G-protein chimera, producing a system that is amenable to high-throughput screening. Screening an in-house terpenoid library, at two screening concentrations (10 mM and 100 mM), has identified four terpenoids (piperonyl alcohol, 1,4-cineole, carvacrol and isoeugenol) that we believe are positive modulators of the southern cattle tick's tyramine receptor.

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Molecular characterization and localization of the first tyramine receptor of the American cockroach (Periplaneta americana)

Cited by 51 publications

References 60 publications

Pharmacological characterization of a tyramine receptor from the southern cattle tick, Rhipicephalus (Boophilus) microplus

Pharmacological characterization of a tyramine receptor from the southern cattle tick, Rhipicephalus (Boophilus) microplus

Characterization of a β-adrenergic-like octopamine receptor from the rice stem borer (Chilo suppressalis)

Interaction of plant essential oil terpenoids with the southern cattle tick tyramine receptor: A potential biopesticide target

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