Characterization of a β-adrenergic-like octopamine receptor from the rice stem borer (<i>Chilo suppressalis</i>)

Wu, Shun‐Fan; Yao, Yao; Huang, Jia; Yè, Gōngyín

doi:10.1242/jeb.068932

Cited by 43 publications

(75 citation statements)

References 37 publications

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“…Moreover, BdOctβR1 exhibited a high expression in the CNS of the adult brain. Similarly, the orthologous receptors were found to be highly expressed in the CNS in many insects such as D. melanogaster [16], C. suppressalis [2], S. gregaria [10], and A. mellifera [17]. This may indicate that octopamine plays a key role in the nerve system as a neurotransmitter.…”

Section: Discussionmentioning

confidence: 99%

“…Among these ligands, mianserin, phentolamine, and chlorpromazine were effective antagonists for OctαRs [20,21,22,23,24], OctβRs [2,3,9], and TyrRs [25], respectively. In our antagonist assays, mianserin was the only chemical with a significant antagonistic activity on BdOctβR1 of B. dorsalis .…”

Section: Discussionmentioning

confidence: 99%

“…In a previous study, phentolamine also acted as a full agonist with little antagonistic effects on DmOctβR2 of D. melanogaster [3] and BmOctβR2 of B. mori [9]. However, phentolamine acted as a partial agonist and had a significant antagonistic effect on CsOctβR2 of C. suppressalis [2]. The fact that phentolamine structurally has an imidazoline ring which is similar to the agonist naphazoline might be the reason why it showed dual effects on different octopamine receptors [2].…”

Section: Discussionmentioning

confidence: 99%

“…It plays a key role in the insect nervous system as a neuromodulator, neurotransmitter, and neurohormone. For example, octopamine modulates behaviors such as aggression, sleep, oviposition, food-seeking, and locomotion [2]. …”

Section: Introductionmentioning

confidence: 99%

“…Two octopamine receptor subtypes have been isolated in Bombyx mori [9] and a partial sequence of a putative OctβR in the desert locust Schistocerca gregaria ( SgOctβR ) has also been cloned [10]. In addition, a β-adrenergic-like octopamine receptor from the rice stem borer ( Chilo suppressalis ) was characterized in a work that also provided evidence that CsOctβR2 is a likely mediator of locomotion [2]. Moreover, it has also been suggested that the octopaminergic system is associated with resource defense, alternative mating tactics, social tolerance, and indirect parental care in Nicrophorus vespilloides [11].…”

Section: Introductionmentioning

confidence: 99%

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Characterization of a β-Adrenergic-Like Octopamine Receptor in the Oriental Fruit Fly, Bactrocera dorsalis (Hendel)

Jiang

Gui

et al. 2016

IJMS

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The biogenic amine octopamine plays a critical role in the regulation of many physiological processes in insects. Octopamine transmits its action through a set of specific G-protein coupled receptors (GPCRs), namely octopamine receptors. Here, we report on a β-adrenergic-like octopamine receptor gene (BdOctβR1) from the oriental fruit fly, Bactrocera dorsalis (Hendel), a destructive agricultural pest that occurs in North America and the Asia-Pacific region. As indicated by RT-qPCR, BdOctβR1 was highly expressed in the central nervous system (CNS) and Malpighian tubules (MT) in the adult flies, suggesting it may undertake important roles in neural signaling in the CNS as well as physiological functions in the MT of this fly. Furthermore, its ligand specificities were tested in a heterologous expression system where BdOctβR1 was expressed in HEK-293 cells. Based on cyclic AMP response assays, we found that BdOctβR1 could be activated by octopamine in a concentration-dependent manner, confirming that this receptor was functional, while tyramine and dopamine had much less potency than octopamine. Naphazoline possessed the highest agonistic activity among the tested agonists. In antagonistic assays, mianserin had the strongest activity and was followed by phentolamine and chlorpromazine. Furthermore, when the flies were kept under starvation, there was a corresponding increase in the transcript level of BdOctβR1, while high or low temperature stress could not induce significant expression changes. The above results suggest that BdOctβR1 may be involved in the regulation of feeding processes in Bactrocera dorsalis and may provide new potential insecticide leads targeting octopamine receptors.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Characterization of a β-Adrenergic-Like Octopamine Receptor in the Oriental Fruit Fly, Bactrocera dorsalis (Hendel)

Jiang

Gui

et al. 2016

IJMS

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Pharmacological characterization of a β‐adrenergic‐like octopamine receptor in Plutella xylostella

Huang

Deng

et al. 2018

Arch Insect Biochem Physiol

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The β-adrenergic-like octopamine receptor (OA2B2) belongs to the class of G-protein coupled receptors. It regulates important physiological functions in insects, thus is potentially a good target for insecticides. In this study, the putative open reading frame sequence of the Pxoa2b2 gene in Plutella xylostella was cloned. Orthologous sequence alignment, phylogenetic tree analysis, and protein sequence analysis all showed that the cloned receptor belongs to the OA2B2 protein family. PxOA2B2 was transiently expressed in HEK-293 cells. It was found that PxOA2B2 could be activated by both octopamine and tyramine, resulting in increased intracellular cyclic AMP (cAMP) levels, whereas dopamine and serotonin were not effective in eliciting cAMP production. Further studies with series of PxOA2B2 agonists and antagonists showed that all four tested agonists (e.g., naphazoline, clonidine, 2-phenylethylamine, and amitraz) could activate the PxOA2B2 receptor, and two of tested antagonists (e.g., phentolamine and mianserin) had significant antagonistic effects. However, antagonist of yohimbine had no effects. Quantitative real-time polymerase chain reaction analysis showed that Pxoa2b2 gene was expressed in all developmental stages of P. xylostella and that the highest expression occurred in male adults. Further analysis with fourth-instar P. xylostella larvae showed that the Pxoa2b2 gene was mainly expressed in Malpighian tubule, epidermal, and head tissues. This study provides both a pharmacological characterization and the gene expression patterns of the OA2B2 in P. xylostella, facilitating further research for insecticides using PxOA2B2 as a target.

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Characterization and functional analysis of a β‐adrenergic‐like octopamine receptor from the oriental armyworm (Mythimna separata Walker)

Guo

Qiu

Chen

et al. 2021

Arch Insect Biochem Physiol

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The β‐adrenergic‐like octopamine receptor (OA2B2), which binds the biogenic amine octopamine, belongs to the class of G‐protein coupled receptors and significantly regulates many physiological and behavioral processes in insects. In this study, the putative open reading frame sequence of the MsOA2B2 gene in Mythimna separata was cloned, the full‐length complementary DNA was 1191 bp and it encoded a 396‐amino acid protein (GenBank accession number MN822800). Orthologous sequence alignment, phylogenetic tree analysis, and protein sequence analysis all showed that the cloned receptor belongs to the OA2B2 protein family. Real‐time quantitative polymerase chain reaction of spatial and temporal expression analysis revealed that the MsOAB2 gene was expressed in all developmental stages of M. separata and was most abundant in egg stages and second and fourth instars compared with other developmental stages, while the expression level during the pupal stage was much lower than that at the other stages. Further analysis with sixth instar M. separata larvae showed that the MsOA2B2 gene was expressed 1.81 times higher in the head than in integument and gut tissues. Dietary ingestion of dsMsOA2B2 significantly reduced the messenger RNA level of MsOA2B2 and decreased mortality following amitraz treatment. This study provides both a pharmacological characterization and the gene expression patterns of OA2B2 in M. separata, facilitating further research for insecticides using MsOA2B2 as a target.

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Characterization of a β-adrenergic-like octopamine receptor from the rice stem borer (Chilo suppressalis)

Cited by 43 publications

References 37 publications

Characterization of a β-Adrenergic-Like Octopamine Receptor in the Oriental Fruit Fly, Bactrocera dorsalis (Hendel)

Characterization of a β-Adrenergic-Like Octopamine Receptor in the Oriental Fruit Fly, Bactrocera dorsalis (Hendel)

Pharmacological characterization of a β‐adrenergic‐like octopamine receptor in Plutella xylostella

Characterization and functional analysis of a β‐adrenergic‐like octopamine receptor from the oriental armyworm (Mythimna separata Walker)

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