Molecular characterization and immune protection of an AN1-like zinc finger protein of Eimeria tenella

Zhao, Huihui; Zhao, Qiping; Zhu, Shanfeng; Huang, Bing; Lv, Ling; Liu, Guiling; Li, Zhihang; Wang, Lu; Dong, Hui

doi:10.1007/s00436-019-06545-x

Cited by 4 publications

(5 citation statements)

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“…Likewise, the rate of invasion inhibition increased with the increase of anti-rEtCHP18905 IgG concentration. Previous studies have shown that polyclonal antibodies can significantly inhibit Spz invading DF-1 cells [7,11,19,48]. In 2016, Zhai et al found that rabbit antiserum against rEtCHP559 can block invasion of host cells by Spz [47].…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization and protective efficacy of a new conserved hypothetical protein ofEimeria tenella

et al. 2021

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Eimeria tenella is an obligate intracellular parasite that actively invades cecal epithelial cells of chickens. This parasite encodes a genome of more than 8000 genes. However, more than 70% of the gene models for this species are currently annotated as hypothetical proteins. In this study, a conserved hypothetical protein gene of E. tenella, designated EtCHP18905, was cloned and identified, and its immune protective effects were evaluated. The open reading frame of EtCHP18905 was 1053bp and encoded a protein of 350 amino acids with a molecular weight of 38.7kDa. The recombinant EtCHP18905 protein (rEtCHP18905) was expressed in E. coli. Using western blot, the recombinant protein was successfully recognized by anti GST-Tag monoclonal antibody and anti-sporozoites protein rabbit serum. Real-time quantitative PCR analysis revealed that the EtCHP18905 mRNA levels were higher in sporozoites than in unsporulated oocysts, sporulated oocysts and second-generation merozoites. Western blot analysis showed that EtCHP18905 protein expression levels were lower in sporozoites than in other stages. Immunofluorescence analysis indicated that the EtCHP18905 protein was located on the surface of sporozoites and second-generation merozoites. Inhibition experiments showed that the ability of sporozoites to invade host cells was significantly decreased after treatment with the anti-rEtCHP18905 polyclonal antibody. Vaccination with rEtCHP18905 protein was able to significantly decrease mean lesion scores and oocyst outputs as compared to non-vaccinated controls. The results suggest that the rEtCHP18905 protein can induce partial immune protection against infection with E. tenella and could be an effective candidate for the development of new vaccines.

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Section: Discussionmentioning

confidence: 99%

Molecular characterization and protective efficacy of a new conserved hypothetical protein ofEimeria tenella

et al. 2021

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“…The antiserum of rHis‐ Et CBS was raised by immunizing six‐week‐old BALB/c mice or two‐month‐old New Zealand white rabbits with 50 or 200 μg of the purified r Et CBS mixed in an equal volume of Freund's Adjuvant as previously reported (Zhao et al, 2020). The polyclonal antibody serum was collected and stored at −80°C for later use.…”

Section: Methodsmentioning

confidence: 99%

“…To evaluate the efficacy of immunization, the average body‐weight gain, fecal oocyst output, and percentage reduction in oocyst excretion were determined at days from 6 to 8 postchallenge, and mean lesion scores were determined at day 8 postchallenge as described previously (Zhai et al, 2016; Zhao et al, 2020). The number of fecal oocysts (oocyst per gram, OPG) was counted using a McMaster chamber.…”

Section: Methodsmentioning

confidence: 99%

Molecular characterization and immune protection by cystathionine β‐synthase from Eimeria tenella

Zhao

Liu

Zhu

et al. 2021

J Eukaryotic Microbiology

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Eimeria tenella is an obligate intracellular apicomplexan parasite that causes avian coccidiosis and leads to severe economic losses in the global poultry industry. Cystathionine β‐synthase (CBS) and cystathionine γ‐lyase (CGL) act together to generate H2S in the reverse transsulfuration pathway. In this study, E. tenella Cystathionine β‐synthase (EtCBS) was cloned using rapid amplification of cDNA 5′‐ends (5'RACE) and characterized, and its immunoprotective effects were evaluated. The recombinant EtCBS protein (rEtCBS) was expressed and successfully recognized by anti‐sporozoites (Spz) protein rabbit serum. EtCBS mRNA levels were highest in Spz by qPCR, and the protein expression levels were higher in unsporulated oocysts (UO) than in other stages by Western blot. Indirect immunofluorescence showed that EtCBS protein was found on the surface of Spz and second‐generation merozoites (Mrz). The invasion inhibition assays showed that rabbit anti‐rEtCBS polyclonal antibodies effectively inhibited parasite invasion host cells. Chickens immunized with rEtCBS protein showed prominently increased weight gains and decreased oocyst output compared to nonimmunized and infected control group. The results suggest that EtCBS could be a potential vaccine candidate against E. tenella.

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“…Another study reported the application of CRISPR for gene function study in E. tenella (Tang et al 2020). Similarly, a study deploying zinc finger-like proteins revealed partial immune-protective effects of AN-1 like proteins of E. tenella (Zhao et al 2020).…”

Section: Opportunities (The Way Forward)mentioning

confidence: 98%

“…Over many decades, Eimeria vaccines have gained a considerable importance, by virtue of effectively preventing the disease without developing resistance in field strains as in anticoccidial chemotherapeutics (Sander et al 2019 ; Liu et al 2020 ; Zhao et al 2020 ). Rather, the sensitivity towards previously resistant chemical anticoccidials has been enhanced by the use of Eimeria vaccines (Jenkins et al 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Vaccines against chicken coccidiosis with particular reference to previous decade: progress, challenges, and opportunities

et al. 2022

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Chicken coccidiosis is an economically significant disease of commercial chicken industry accounting for losses of more than £10.4 billion (according to 2016 prices). Additionally, the costs incurred in prophylaxis and therapeutics against chicken coccidiosis in developing countries (for instance Pakistan according to 2018 prices) reached US $45,000.00 while production losses for various categories of chicken ranges 104.74 to US $2,750,779.00. The infection has been reported from all types of commercial chickens (broiler, layer, breeder) having a range of reported prevalence of 7–90%. The concern of resistance towards major anticoccidials has provided a way forward to vaccine research and development. For prophylaxis of chicken coccidiosis, live virulent, attenuated, ionophore tolerant strains and recombinant vaccines have been extensively trialed and commercialized. Eimeria antigens and novel vaccine adjuvants have elicited the protective efficacy against coccidial challenge. The cost of production and achieving robust immune responses in birds are major challenges for commercial vaccine production. In the future, research should be focused on the development of multivalent anticoccidial vaccines for commercial poultry. Efforts should also be made on the discovery of novel antigens for incorporation into vaccine designs which might be more effective against multiple Eimeria species. This review presents a recap to the overall progress against chicken Eimeria with particular reference to previous decade. The article presents critical analysis of potential areas for future research in chicken Eimeria vaccine development.

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Molecular characterization and immune protection of an AN1-like zinc finger protein of Eimeria tenella

Cited by 4 publications

References 64 publications

Molecular characterization and protective efficacy of a new conserved hypothetical protein ofEimeria tenella

Molecular characterization and protective efficacy of a new conserved hypothetical protein ofEimeria tenella

Molecular characterization and immune protection by cystathionine β‐synthase from Eimeria tenella

Vaccines against chicken coccidiosis with particular reference to previous decade: progress, challenges, and opportunities

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