Molecular Characterization and Genotypic Identification of Metschnikowia Species

Lopandić, Ksenija; Prillinger, Hansjörg; Molnár, Orsolya; Giménez-Jurado, Gabriella

doi:10.1016/s0723-2020(96)80068-3

Cited by 30 publications

(16 citation statements)

References 21 publications

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“…In contrast to the findings of various authors (e.g. Messner et al, 1994 ;Baleiras Couto et al, 1994 ;Mollnar et al, 1995 ;Lopandic et al, 1996 ;Romano et al, 1996 ;Prillinger et al, 1999 ;Naumova et al, 2000 ;Paramithiotis et al, 2000), we conclude that RAPD-PCR is not a reliable method for the determination or comparison of unknown yeast strains. We agree with de Barros Lopes et al (1998) that strains producing distinct amplification patterns cannot be assumed to be different species, given that members of a species may be represented by several banding patterns.…”

Section: Molecular Characterizationcontrasting

confidence: 99%

“…The following primers were synthesized by MWG-Biotech : 1, 5h-ACGGTCTTGG3h ; 2, 5h-TGCAGCGTGG-3h ; 3, 5h-GGGTAACGCC-3h. The primers were as suggested by Lopandic et al (1996). The PCR protocol used was as follows.…”

Section: Methodsmentioning

confidence: 99%

“…Messner et al, 1994 ;Mollna! r et al, 1995 ;Lopandic et al, 1996 ;Romano et al, 1996 ;Prillinger et al, 1999 ;Ganter & de Barros Lopes, 2000 ;Paramithiotis et al, 2000 ;Naumova et al, 2000) have been used frequently in the characterization and determination of yeast strains.…”

Section: Introductionmentioning

confidence: 99%

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Conflicting results obtained by RAPD-PCR and large-subunit rDNA sequences in determining and comparing yeast strains isolated from flowers: a comparison of two methods

Herzberg¹

2002

International Journal of Systematic and Evolutionary Microbiology

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Sixty-six yeast strains isolated from the nectar of various plant species in Central Europe were characterized by randomly amplified polymorphic DNA PCR (RAPD-PCR) and by sequencing of the variable D1/D2 domain of large-subunit (26S) rDNA. The usefulness of both methods for the determination and comparison of unknown ascomycetous and basidiomycetous yeast strains was compared and evaluated. The reproducibility of RAPD-PCR was shown to be low and the information obtained by this method was clearly not as precise as that obtained from sequence analysis. Numerous imponderables make RAPD-PCR analysis unreliable, at least as a means of identifying yeasts in ecological studies. The lack of standard protocols for RAPD-PCR analysis and the absence of a general database of banding patterns made it impossible to identify unknown yeast strains or to recognize new species. In contrast to RAPD-PCR, sequence analysis of the D1/D2 domain was found to be a fast and reliable method for the rapid identification of yeast species and was also shown to be an invaluable tool for the discovery of new species.

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Section: Molecular Characterizationcontrasting

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Conflicting results obtained by RAPD-PCR and large-subunit rDNA sequences in determining and comparing yeast strains isolated from flowers: a comparison of two methods

Herzberg¹

2002

International Journal of Systematic and Evolutionary Microbiology

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“…Further analyses are needed to elucidate the functional role of chlamydospores in Metschnikowia. Resistance to Pichia membranifaciens mycocins (Golubev, 2011) and carbohydrate composition of cell walls (Lopandic et al, 1996) have been also suggested as differential characters between the two main ecological Metschnikowia groups (aquatic vs. terrestrial). Limited sampling (nine and 12 species respectively) in both studies prevents us from testing these hypotheses in a phylogenetic framework.…”

Section: Evolutionary Trends Of Morphological Key Characters Within Mmentioning

confidence: 99%

Phylogenetic analysis of the angiosperm-floricolous insect–yeast association: Have yeast and angiosperm lineages co-diversified?

Guzmán

Lachance

Herrera

2013

Molecular Phylogenetics and Evolution

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a b s t r a c tMetschnikowia (Saccharomycetales, Metschnikowiaceae/ Metschnikowia clade) is an ascomycetous yeast genus whose species are associated mostly with angiosperms and their insect pollinators over all continents. The wide distribution of the genus, its association with angiosperm flowers, and the fact that it includes some of the best-studied yeasts in terms of biogeography and ecology makeMetschnikowia an excellent group to investigate a possible co-radiation with angiosperm lineages. We performed phylogenetic analyses implementing Bayesian inference and likelihood methods, using a concatenated matrix (%2.6 Kbp) of nuclear DNA ( ACT1, 1st and 2nd codon positions of EF2, Mcm7, and RPB2) sequences. We included 77 species representing approximately 90% of the species in the family. Bayesian and parsimony methods were used to perform ancestral character reconstructions within Metschnikowia in three key morphological characters. Patterns of evolution of yeast habitats and divergence times were explored in the Metschnikowia clade lineages with the purpose of inferring the time of origin of angiosperm-associated habitats within Metschnikowiaceae. This paper presents the first phylogenetic hypothesis to include nearly all known species in the family. The polyphyletic nature of Clavispora was confirmed and Metschnikowia species (and their anamorphs) were shown to form two groups: one that includes mostly floricolous, insect-associated species distributed in mostly tropical areas (the large-spored Metschnikowia clade and relatives) and another that comprises more heterogeneous species in terms of habitat and geographical distribution. Reconstruction of character evolution suggests that sexual characters (ascospore length, number of ascospores, and ascus formation) evolved multiple times within Metschnikowia. Complex and dynamic habitat transitions seem to have punctuated the course of evolution of the Metschnikowiaceae with repeated and independent origins of angiosperm-associated habitats. The origin of the family is placed in the Late Cretaceous (71.7 Ma) with most extant species arising from the Early Eocene. Therefore, the Metschnikowiaceae likely radiated long after the Mid-Cretaceous radiations of angiosperms and their diversification seems to be driven by repeated radiation on a pre-existing diverse resource.

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“…Vitamin requirements were tested after at least two consecutive inoculations on vitamin-free medium. The qualitative and quantitative monosaccharide pattern of purified yeast cell walls was determined after trifluoracetic acid hydrolysis according to the method of Dörfler (1990) with some modifications as described by Lopandic et al (1996).…”

Section: Phenotypic Characterisationmentioning

confidence: 99%

Schizonella caricis-atratae (Ustilaginomycetes): a new cryptic species on Carex atrata from Austria

et al. 2009

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Based on DNA-fingerprinting, ITS-DNA sequencing and physiological characteristics, a new species of Schizonella, Sch. caricis-atratae, is described. Sch. caricisatratae is a so-called cryptic species, i.e. morphologically it is identical to Sch. melanogramma, but differs in physiological and molecular phylogenetic characters. Sch. caricisatratae can be distinguished by the following tests: growth on myo-inositol, D,L-lactate, salicine, maltose, αmethyl-Dglucoside, butane-2,3-diol, and D-turanose, at 30°C; no growth on galactose. Sch. caricis-atratae can be isolated from Carex atrata. The type strain is HB 3, CBS 123477.

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Molecular Characterization and Genotypic Identification of Metschnikowia Species

Cited by 30 publications

References 21 publications

Conflicting results obtained by RAPD-PCR and large-subunit rDNA sequences in determining and comparing yeast strains isolated from flowers: a comparison of two methods

Conflicting results obtained by RAPD-PCR and large-subunit rDNA sequences in determining and comparing yeast strains isolated from flowers: a comparison of two methods

Phylogenetic analysis of the angiosperm-floricolous insect–yeast association: Have yeast and angiosperm lineages co-diversified?

Schizonella caricis-atratae (Ustilaginomycetes): a new cryptic species on Carex atrata from Austria

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