Molecular characterization and genetic variability studies associated with fruit quality of indigenous mango (Mangifera indica L.) cultivars

Start codon targeted polymorphism for evaluation of functional genetic variation and relationships in cultivated castor (Ricinus communis L.) genotypes

“…HUANG et al (2014) assessed the genetic diversity of six Hemarthria cultivars using seven SCoT primers, which together amplified 105 bands with an average of 15 bands per sample. Start codon-targeted markers were utilized by GAJERA et al (2014) who used 19 SCoT markers for characterization and genetic comparison among 20 mango cultivars. These primers produced total 117 loci across 20 cultivars, of which 96 (79.57 %) were polymorphic.…”

“…In principle, SCoT is similar to RAPD and ISSR because the same single primer is used as the forward and reverse primer (COLLARD and MACKILL, 2009). Suitability of SCoT markers system has been successfully employed in genetic diversity analysis and fingerprinting of a number of agricultural and horticultural crop species, such as oat (BALÁŽOVÁ et al, 2017), rye (PETROVIČOVÁ et al, 2017), maize (VIVODÍK et al, 2016, date palm (AL-QURAINY et al, 2015), orchardgrass (JIANG et al, 2014), pepper (TSABALLA et al, 2015, ramie (SATYA et al, 2015), castor (KALLAMADIA et al, 2015), sugarcane (QUE et al, 2014) and mango (GAJERA et al, 2014).…”

Start codon targeted (SCoT) polymorphism reveals genetic diversity of Manilkara in Thailand

“…An innovative molecular marker known as Start Codon Targeted (SCoT) polymorphism targets on short ATG start codon in plant genes has been reported (Collard and Mackill 2009;Satya et al 2016;Jedrzejczyk 2020). It has several advantages over RAPD, ISSR, and AFLP, as it is more consistent, produce more reproducible and reliable bands and can be used effectively for population studies, genetic mapping in different plants and in the marker-assisted selection programs (Gorji et al 2011;Xiong et al 2011;Alikhani et al 2014;Gajera et al 2014;Yue et al 2019). Similar to RAPD and ISSR markers, SCoT are important markers that could be used for different genetic application such as, to assess genetic diversity and structure, in bulk segregation analyses, quantitative trait loci (QTL) mapping and DNA fingerprinting (Luo et al 2012;Gao et al 2014;Emami et al 2018;Gupta et al 2019).…”

Origen, dispersión y diversidad del chirimoyo (Annona cherimola Mill.) en el continente americano

“…Otros marcadores basados en PCR son los marcadores de intersecuencia simple (Inter Simple Sequence Repeat) (ISSR), aplicados, por ejemplo, en higuera (Amel et al 2004;2005;Ikegami et al 2009) o clementina (Breto et al 2001 (Gajera et al 2014;Luo et al 2011). Otros, como polimorfismo amplificado de secuencias relacionadas (Sequence Related Amplified Polymorphism) (SRAP) ha sido usado en albaricoquero (Li et al 2014c), caqui (Jing et al 2013a) o almendro (Jing et al 2013b), así como los polimorfismos para la amplificación de regiones blanco (Target Region Amplification Polymorphism) (TRAP) que se han usado en manzano (Guo et al 2009).…”

“…(Crowhurst et al 2008). Other less used molecular markers include start codon targeted polymorphisms (SCoT) that uses single primers designed to anneal to the flanking regions of the ATG initiation codon on both DNA strands and has been used in Mangifera indica (Gajera et al 2014;Luo et al 2011) and SRAP and TRAP, which stand for sequence-related amplified polymorphism and targeted region amplified polymorphism, respectively, that have been used in several fruit tree species. In the case of SRAP, recent works in fruit trees include apricot (Li et al 2014c), persimmon (Jing et al 2013a), or almond (Jing et al 2013b), and, in the case of TRAP, apple (Guo et al 2009).…”