Molecular Characterization and Genetic Diversity of Yellow Passion Fruit Based on RAPD Markers

Campos, Angélica Vieira Sousa; Peixoto, José Ricardo; Faleiro, Fábio Gelape; Vilela, Michelle Souza; Pires, Márcio de Carvalho

doi:10.5539/jas.v11n3p575

Cited by 4 publications

(4 citation statements)

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“…Traditionally, diversity in Passiflora is estimated by observing variations in morphological or agronomic characteristics or tolerance to biotic or abiotic factors, which does not reflect the genetic relationship between evaluated materials and is affected by environmental changes and dependence on the stage of development (Pereira et al, 2015). DNA markers overcome these limitations and are a useful tool for the evaluation and characterization of germplasm (Vieira et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…The genetic diversity in Passiflora has been studied with different molecular techniques, such as random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and sequence-related amplified polymorphism (SRAP) (Oluoch et al, 2018;Vieira et al, 2019); however, simple sequence repeat (SSR) markers are the most used techniques for the characterization of germplasm in different crops since they are highly polymorphic, reproducible and co-dominant, with wide genome coverage (Gramazio et al, 2018). Among the microsatellite markers, inter-simple sequence repeat (ISSR), which combines characteristics of the RAPD and microsatellites techniques, facilitates multilocus genomic analysis with a single primer based on regions of SSR, presents good reproducibility, has a low cost, is simple, does not need previous genome information (Morillo et al, 2018), and has proven to be polymorphic and informative in studies on genetic diversity in Passiflora (Santos et al, 2011;Sousa et al, 2015;Batista et al, 2017;Oliveira et al, 2019;Vianna et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of the genetic diversity in Passiflora spp. in the Boyacá Department, Colombia

Martínez

Morillo

Reyes-Ardila

2020

Chil. j. agric. res.

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Passiflora is a group of species of economic importance in Colombia because of their productive potential and nutritional, pharmaceutical and industrial properties; therefore, it is necessary to study the genetic diversity of the species cultivated in principal productive departments, such as Boyacá. The use of molecular markers has become a fundamental tool for germplasm characterization, which effectively complements morphological and agronomic information. This research aimed to characterize the genetic diversity of Passiflora spp. grown in the Department of Boyacá using inter-simple sequence repeat (ISSR) markers. The eight ISSRs generated a total 138 bands with molecular weights between 250 and 1350 kb. With a similarity coefficient of 0.60, seven groups were formed, showing lax distribution of the individuals. The percentage of polymorphic loci was between 56% (TG) and 90% (AG). The average value of heterozygosity was 0.56 with a genetic differentiation coefficient (FST) of 0.16, which indicated great genetic diversity but without a defined population structure. The AMOVA showed that 64% of the observed genetic variation was due to the component within the groups. The results suggested levels of hierarchy and subdivision finer than those considered in this study, in addition to a complementary morphoagronomic characterization that established the total genetic diversity, which can be used to implement a breeding program for more productive cultivars that are resistant to the principal phytosanitary problems.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of the genetic diversity in Passiflora spp. in the Boyacá Department, Colombia

Martínez

Morillo

Reyes-Ardila

2020

Chil. j. agric. res.

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“…As an alternative, molecular marker utilizations for plant identification have been widely accepted due to several advantages, such as unlimited number, unaffected by environment and growing conditions, easy interpretation, and reliable repeatable results (Antunes et al, 1997). Several studies have been reported on the genetic diversity of Passiflora species with random amplified polymorphic DNA (RAPD) (Vieira et al, 2019), amplified fragments length polymorphism markers (AFLP) (Ortiz et al, 2012), simple sequence repeat markers (SSR) (Araya et al, 2017;Grisi et al, 2019), inter-simple sequence repeats (ISSR) (Martinez et al, 2020) and sequence-related amplified polymorphism (SRAP) markers (Oluoch et al, 2018). Among these, the ISSR method is preferred for use in plants with limited prior genetic knowledge, as it is a simple, fast, relatively cheap technique, requires minimal laboratory skills, small amounts of DNA, and generates a large number of fragments in each reaction (Vianna et al, 2019).…”

Section: Issr Amplificationmentioning

confidence: 99%

Molecular characterization of Passiflora edulis f. flavicarpa Degener with ISSRs markers

Morillo,

Muñoz,

Morillo

2023

Braz. J. Biol.

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Passiflora edulis it is a specie widely distributed and cultivated in Colombia, with economic potential. Although there is a wide genetic and phenotypic variability, it has not yet been explored through the use of molecular techniques. This study aimed to characterize the structure and genetic diversity of P. edulis cultivars using ISSR markers. The study was carried out using leaf samples from 21 cultivars of P. edulis collected within a productive system in the department of Boyacá, Colombia, using seven ISSR primers. Genetic similarity was used to cluster by the UPGMA method, polymorphic information content (PIC), expected heterozygosity (He), Shannon index (I), gene flow (Nm), and coefficient of genetic differentiation (Gst) were estimated using POPGENE and TFPGA software. The Bayesian model and analysis of molecular variance (AMOVA) were used to assess the genetic structure. Cultivars of P. edulis showed high polymorphism rates. Seven ISSR produced 138 loci. The cluster analysis formed two groups according to the genetic similarity and phenotypic characteristics associated mainly with the fruit. The average value of expected heterozygosity was 0.29 for the total population and 0.27 and 0.22 for groups I and II, respectively. AMOVA indicates higher diversity within groups, but not between groups showing levels of hierarchy different from those considered in this study. Moderate genetic differentiation (Gst=0.12) and high gene flow (Nm=3.91) are observed.

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“…The knowledge generated by genetic based characterization offers information for the future breeding efforts and other aspects of biochemical and ecological studies. Several studies have been reported on the genetic diversity of Passiflora species with random amplified polymorphic DNA (RAPD) (Vieira et al, 2019), amplified fragments length polymorphism markers (AFLP) (Ortiz et al, 2012), simple sequence repeat markers (SSR) (Oliveira et al, 2005;Pérez and d'Eeckenbrugge, 2017;Araya et al, 2017;Grisi, 2019;Vianna et al, 2019), inter-simple sequence repeats (ISSR) (dos Santos et al, 2011;Vianna et al, 2019) and sequence-related amplified polymorphism (SRAP) markers (Oluoch et al, 2018). Thus, the main objective of this study was to assess the genetic variability and decrypt the population structure of purple passion fruit accessions Chepkoech et al 203 grown within in Uasin Gishu County, Kenya.…”

Section: Introductionmentioning

confidence: 99%

Assessment of genetic variability of passion fruit using simple sequence repeat (SSR) markers

Alkamoi¹,

Rotich²,

Chepkoech³

2020

J. Agric. Sci. Pract.

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Purple passion fruit (Passiflora edulis Sims) is the third most important fruit crop in Kenya that is produced for both local and export markets. In Uasin Gishu County, passion fruit had recently emerged as an important cash crop for the small-holder farmers. Understanding the structure and diversity of species is very important in plant breeding and in conservation of genetic resources related activities. This study was set out in 2017-2018 to determine the genetic diversity of purple passion fruits genotypes grown in Uasin Gishu County, Kenya using SSR markers. Among the 50 purple passion fruit accessions used in this study, the genetic distance coefficients among accessions ranged from 0.24 to 0.72, with an average of 0.48. The results of STRUCTURE analysis suggested that the 50 accessions could be grouped into five sub-populations. The clustering was based on the unweighted pair-group method of arithmetic averages (UPGMA) where accessions were divided into three major clusters. The UPGMA dendrogram revealed that accessions from identical or adjacent areas were generally, but not entirely, clustered into the same cluster. Comparison of the UPGMA dendrogram and the Bayesian STRUCTURE analysis showed general agreement between the population sub-divisions and the genetic relationships among accessions. Principal coordinate analysis (PCoA) with SSR markers revealed a similar grouping of accessions to the UPGMA dendrogram and STRUCTURE analysis. Analysis of molecular variance (AMOVA) indicated that 16% of the total was attributed to the diversity among sub-populations, while 84% was associated with differences within sub-populations. Overall, there was a considerable amount of genetic variability among passion fruit accessions grown in Uasin Gishu County of Kenya. The study represents the comprehensive investigation of the genetic diversity of passion fruit accessions which would be valuable for germplasm collection, genetic improvement, and efficient utilization.

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Molecular Characterization and Genetic Diversity of Yellow Passion Fruit Based on RAPD Markers

Cited by 4 publications

References 4 publications

Characterization of the genetic diversity in Passiflora spp. in the Boyacá Department, Colombia

Characterization of the genetic diversity in Passiflora spp. in the Boyacá Department, Colombia

Molecular characterization of Passiflora edulis f. flavicarpa Degener with ISSRs markers

Assessment of genetic variability of passion fruit using simple sequence repeat (SSR) markers

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