Molecular characterization and gene expression of the pituitary adenylate cyclase-activating polypeptide (PACAP) in the lizard brain

Valiante, Salvatore; Prisco, Marina; Capaldo, Anna; Zambrano, Immacolata; Falco, Maria De; Andreuccetti, Piero; Laforgia, Vincenza; Varano, Lorenzo

doi:10.1016/j.brainres.2006.09.103

Cited by 17 publications

(14 citation statements)

References 37 publications

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“…The presence of VIP in chromaffin tissue, and VIP-stimulated release of catecholamines and aldosterone in vivo, was previously shown in the Italian wall lizard Podarcis sicula (Laforgia et al 1999). It has also been demonstrated that VIP administration enhances the shift from noradrenaline to adrenaline production in the chromaffin cells (De Falco et al 2003); further, we cloned lizard PACAP and showed that it is produced and widely expressed by P. sicula brain (Valiante et al 2007).…”

Section: Introductionmentioning

confidence: 82%

“…Furthermore, recent studies have shown that the in vivo administration of exogenous VIP acts on adrenal glands of P. sicula, enhancing adrenaline production (De Falco et al 2003). Further, the evolutionary conservation of PACAP in P. sicula (Valiante et al 2007) suggests an important role of these neuropeptides in the physiology of reptiles.…”

Section: Discussionmentioning

confidence: 99%

“…The immunohistochemistry procedure was described elsewhere (Valiante et al 2007). Briefly, antigen unmasking was carried out in 10 mM citrate buffer (pH 6 .…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…They were complementary to the lizard PACAP (Valiante et al 2007), human VPAC 2 and mouse VPAC 1 cDNA were kindly provided by P Robberecht and M S O'Dorisio respectively (Svoboda et al 1994, Karacay et al 2001. Briefly, after transfection of JM109 cells, plasmids were recovered with Ultraclean miniplasmid kit (MoBIO Labs, Carlsbad, CA, USA) and used for an in vitro transcription reaction to synthesize both sense and antisense RNA probes of 300 bp each.…”

Section: In Situ Hybridizationmentioning

confidence: 99%

“…Briefly, after transfection of JM109 cells, plasmids were recovered with Ultraclean miniplasmid kit (MoBIO Labs, Carlsbad, CA, USA) and used for an in vitro transcription reaction to synthesize both sense and antisense RNA probes of 300 bp each. In situ hybridization procedure was performed as described elsewhere (Valiante et al 2007). All solutions used in in situ hybridization were made with bidistilled water treated with 0 .…”

Section: In Situ Hybridizationmentioning

confidence: 99%

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Pituitary adenylate cyclase-activating polypeptide, vasoactive intestinal polypeptide and their receptors: distribution and involvement in the secretion of Podarcis sicula adrenal gland

Valiante¹,

Prisco²,

Sciarrillo³

et al. 2007

Journal of Endocrinology

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Vasoactive intestinal polypeptide (VIP) and pituitary adenylate cyclase-activating polypeptide (PACAP) are regulatory neuropeptides of the hypothalamus-hypophyseal-adrenal axis, acting via the common receptors VPAC 1 and VPAC 2 and the selective PACAP receptor PAC 1 . In the adrenal glands of the Italian wall lizard, Podarcis sicula, the presence of VIP in chromaffin cells, and the VIP-stimulated release of catecholamine and aldosterone in vivo, was previously shown. To examine the localization of both peptides and receptors and their mRNAs in the adrenal gland of P. sicula, immunohistochemistry and in situ hybridization were performed: PACAP and its mRNA were detected in chromaffin cells, VPAC 1 was found associated with steroidogenic tissue, VPAC 2 and PAC 1 with chromaffin tissue. Using 'far western blot' technique, we showed the presence of specific binding sites for VIP/PACAP in the adrenal glands of the lizard. The effects of both VIP and PACAP on the adrenal cells of the lizard were examined in vitro in adrenal cell co-cultures: both VIP and PACAP enhanced catecholamine, corticosterone and aldosterone release from adrenal cell co-culture in a time-and dose-dependent manner. The catecholamine release was inhibited by PAC 1 antagonist and in VPAC 2 immunoneutralized adrenal cells. The effects of VIP and PACAP on aldosterone secretion were counteracted by VPAC 1 antagonist administration in vitro. Corticosterone secretion elicited by VIP was not blocked by VPAC 1 antagonist, while the PACAP-induced release of corticosterone was blocked by the antagonist. Overall, our investigations indicate that these neuropeptides of the secretin superfamily can act not only as neurotransmitters but also as autocrine and paracrine regulators on chromaffin and cortical cells, being important mediators of the non-cholinergic system in the lizard adrenal gland.

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Section: Introductionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

“…The immunohistochemistry procedure was described elsewhere (Valiante et al 2007). Briefly, antigen unmasking was carried out in 10 mM citrate buffer (pH 6 .…”

Section: Immunohistochemistrymentioning

confidence: 99%

Section: In Situ Hybridizationmentioning

confidence: 99%

Section: In Situ Hybridizationmentioning

confidence: 99%

See 3 more Smart Citations

Pituitary adenylate cyclase-activating polypeptide, vasoactive intestinal polypeptide and their receptors: distribution and involvement in the secretion of Podarcis sicula adrenal gland

Valiante¹,

Prisco²,

Sciarrillo³

et al. 2007

Journal of Endocrinology

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Molecular cloning of VIP and distribution of VIP/VPACR system in the testis ofPodarcis sicula

et al. 2014

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Using molecular, biochemical, and cytological tools, we studied the nucleotide and the deduced amino acid sequence of PHI/VIP and the distribution of VIP/VPAC receptor system in the testis of the Italian wall lizard Podarcis sicula to evaluate the involvement of such a neuropeptide in the spermatogenesis control. We demonstrated that (1) Podarcis sicula VIP had a high identity with other vertebrate VIP sequences, (2) differently from mammals, VIP was synthesized directly in the testis, and (3) VIP and its receptor VPAC2 were widely distributed in germ and somatic cells, while the VPAC1 R had a distribution limited to Leydig cells. Our results demonstrated that in Podarcis sicula the VIP sequence is highly preserved and that this neuropeptide is involved in lizard spermatogenesis and steroidogenesis.

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Sex steroid hormone secretion in the wall lizard Podarcis sicula testis: The involvement of VIP

et al. 2015

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Vasoactive intestinal peptide (VIP) is a pleiotropic neuropeptide involved in different functions, including testosterone synthesis. Recently, we reported the presence of VIP in the testis of Podarcis sicula, throughout the reproductive cycle. Now, we investigated the effects of the VIP on steroidogenesis in significant periods of the Podarcis reproductive cycle: winter stasis, reproductive period, and summer stasis. Using VIP treatments in testis culture in absence or presence of receptors antagonists, we demonstrated for the first time that in P. sicula, VIP is involved not only in testosterone synthesis, as in mammals, but in 17β-estradiol synthesis too. J. Exp. Zool. 323A: 714-721, 2015. © 2015 Wiley Periodicals, Inc.

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Molecular characterization and gene expression of the pituitary adenylate cyclase-activating polypeptide (PACAP) in the lizard brain

Cited by 17 publications

References 37 publications

Pituitary adenylate cyclase-activating polypeptide, vasoactive intestinal polypeptide and their receptors: distribution and involvement in the secretion of Podarcis sicula adrenal gland

Pituitary adenylate cyclase-activating polypeptide, vasoactive intestinal polypeptide and their receptors: distribution and involvement in the secretion of Podarcis sicula adrenal gland

Molecular cloning of VIP and distribution of VIP/VPACR system in the testis ofPodarcis sicula

Sex steroid hormone secretion in the wall lizard Podarcis sicula testis: The involvement of VIP

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