Molecular Characterization and Expression of OfJNK and Ofp38 in Ostrinia furnacalis (Guenée) Under Different Environmental Stressors

Su, Li; Meng, Jian‐Yu; Yang, Hong; Zhang, Changyu

doi:10.3389/fphys.2020.00125

Cited by 5 publications

(13 citation statements)

References 40 publications

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“…In addition, AKT is an excellent connector molecule that regulates the interaction between P38 and JUN pathway (Cuadrado & Nebreda 2010). Currently, the expression responses of MAPK pathway genes have been analyzed under different environmental stimuli such as high temperature and UV irradiation in several insects (Wu & Cho 2014;Su et al 2020). However, the MAPK pathway genes in predatory mites has not been systematically studied.…”

Section: Discussionmentioning

confidence: 99%

“…The mitogen-activated protein kinase (MAPK) signaling pathway has significant importance for decoding external stimuli into adaptive responses and signal transduction in eukaryotes (Cowan & Storey 2003). Many studies have shown that MAPKs can be activated by various environmental stress stimuli including heat stress and regulate the physiological processes from gene expression to cell death (Su et al 2020; Tafesh-Edwards & Eleftherianos 2020). The MAPK signaling pathway is mainly composed of three subfamilies including C-Jun N-terminal kinase (JNK), p38 and extracellular signal-regulated kinase (ERK).…”

Section: Introductionmentioning

confidence: 99%

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Activation of MAPK signaling pathway genes leads to promoted thermotolerance in a high-temperature adapted predatory mite Neoseiulus barkeri (Hughes)

Tian

Wang

et al. 2023

saa

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Predatory mites are important biological control agents for phytophagous mites and several small insect pests on vegetables, while the control efficiency is often limited by the changeable or fluctuating environment, especially under the threat of global warming. To mitigate the undesired impacts of high temperature environment, a high-temperature adapted strain (HTAS) of the predatory mite Neoseiulus barkeri (Hughes) was screened from a conventional strain (CS) by long-term heat acclimation (35°C) and regular heat hardenings (45°C). In this study, to investigate the molecular mechanisms of enhanced thermotolerance in HTAS N. barkeri, four mitogen-activated protein kinase (MAPK) pathway genes were identified from the predatory mite N. barkeri. The expression levels at different developmental stages showed four MAPK pathway genes were highest at adults and lowest at eggs or nymphs in both strains. Under 42℃ high temperature stress, the expression levels of four identified MAPK pathway genes increased rapidly at a short time in both N. barkeri strains and the magnitude of expression increase in HTAS N. barkeri was even more evident. In addition, the MAPK pathway genes expression in CS N. barkeri sharply decreased following longer exposure periods at 4 h, whereas the expression in HTAS N. barkeri remained elevated after 4 h. These results suggested that MAPK signaling pathway participated in the formation of thermotolerance in predatory phytoseiid mites, which provided new insights in promoting the biological control efficiency of predatory mites under changeable environment.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Activation of MAPK signaling pathway genes leads to promoted thermotolerance in a high-temperature adapted predatory mite Neoseiulus barkeri (Hughes)

Tian

Wang

et al. 2023

saa

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“…Insects sense the external environment stimulation and transmit the signal to the body to respond to environmental stresses 43 . Most insects have developed various mechanisms, such as morphological and physiological adaptations, to cope with environmental stresses, such as high temperature, cold stress, UV radiation, and bacterial and fungal infections 32,[77][78][79] . Solar UV radiation is one of the most omnipresent environmental stresses 41,42 .…”

Section: Discussionmentioning

confidence: 99%

“…Our previous study reported that JNK in O. furnacalis shows increased expression after UV-A exposure for 60 min. RasGAP and JNK are upregulated in O. furnacalis under UV-A stress, indicating that MAPK pathways in O. furnacalis might involve UV-A exposure-induced stress responses 32 .…”

Section: Discussionmentioning

confidence: 99%

“…UV-A is used as black light for forecasting and controlling O. furnacalis and directly affects the behavior and visual physiology of O. furnacalis 29 . Studies also have reported the effects of UV-A on the proteome, reproductive system, and signal pathway proteins of O. furnacalis [30][31][32] . Therefore, O. furnacalis might have specific adaptive strategies for responding to UV-A stress in order to better adapt to environmental stresses.…”

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confidence: 99%

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Comparative transcriptome and metabolome analysis of Ostrinia furnacalis female adults under UV-A exposure

Yang

Meng

et al. 2021

Sci Rep

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Ultraviolet A (UV-A) radiation is a significant environmental factor that causes photoreceptor damage, apoptosis, and oxidative stress in insects. Ostrinia furnacalis is an important pest of corn. To understand the adaptation mechanisms of insect response to UV-A exposure, this study revealed differentially expressed genes (DEGs) and differently expressed metabolites (DEMs) in O. furnacalis under UV-A exposure. Three complementary DNA libraries were constructed from O. furnacalis adult females (CK, UV1h, and UV2h), and 50,106 expressed genes were obtained through Illumina sequencing. Of these, 157 and 637 DEGs were detected in UV1h and UV2h after UV-A exposure for 1 and 2 h, respectively, compared to CK, with 103 and 444 upregulated and 54 and 193 downregulated genes, respectively. Forty four DEGs were detected in UV2h compared to UV1h. Comparative transcriptome analysis between UV-treated and control groups revealed signal transduction, detoxification and stress response, immune defense, and antioxidative system involvement. Metabolomics analysis showed that 181 (UV1h vs. CK), 111 (UV2h vs. CK), and 34 (UV2h vs. UV1h) DEMs were obtained in positive ion mode, while 135 (UV1h vs. CK), 93 (UV2h vs. CK), and 36 (UV2h vs. UV1h) DEMs were obtained in negative ion mode. Moreover, UV-A exposure disturbed amino acid, sugar, and lipid metabolism. These findings provide insight for further studies on how insects protect themselves under UV-A stress.

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Identification and characteristic analysis of an extracellular signal‐regulated kinase from Ostrinia furnacalis Guenée

Li,

Xu,

Zhang

et al. 2023

Arch Insect Biochem Physiol

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The extracellular signal‐regulated kinase (ERK) pathway, a critical genetic determinant, controls diverse physiological functions, including innate immunity, development, and stress response. In the current study, a full‐length cDNA (1592bp) encoding the ERK gene (OfERK) was cloned from Ostrinia furnacalis Guenée (GenBank accession number: MF797866). The open reading frame of the OfERK gene encoded 364 amino acids and shared 96.43%–98.08% amino acid identities with other insect mitogen‐activated protein kinases. For spatiotemporal analysis of the expression pattern, OfERK exhibited a significant peak expression on the 3rd day of the pupa stage and showed the highest expression in hemocytes specifically. Indirect immunofluorescence assays and immuno‐electron microscopy revealed a wide distribution of the OfERK protein in hemocytes and epidermis. Moreover, the results demonstrated that the Bt Cry1Ab‐activated toxin significantly induces the expression of OfERK. Other genes related to immune response, development, and stress response exhibited dynamic changes in expression after Cry1Ab oral treatment. The expression of OfERK was downregulated through RNA interference, and the correlation of its expression with other related genes was verified using quantitative real‐time polymerase chain reaction. Our study provides valuable insights into the regulatory mechanism of ERK in insects for future studies.

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Molecular Characterization and Expression of OfJNK and Ofp38 in Ostrinia furnacalis (Guenée) Under Different Environmental Stressors

Cited by 5 publications

References 40 publications

Activation of MAPK signaling pathway genes leads to promoted thermotolerance in a high-temperature adapted predatory mite Neoseiulus barkeri (Hughes)

Activation of MAPK signaling pathway genes leads to promoted thermotolerance in a high-temperature adapted predatory mite Neoseiulus barkeri (Hughes)

Comparative transcriptome and metabolome analysis of Ostrinia furnacalis female adults under UV-A exposure

Identification and characteristic analysis of an extracellular signal‐regulated kinase from Ostrinia furnacalis Guenée

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