Comparative transcriptome and metabolome analysis of Ostrinia furnacalis female adults under UV-A exposure

Abstract

Ultraviolet A (UV-A) radiation is a significant environmental factor that causes photoreceptor damage, apoptosis, and oxidative stress in insects. Ostrinia furnacalis is an important pest of corn. To understand the adaptation mechanisms of insect response to UV-A exposure, this study revealed differentially expressed genes (DEGs) and differently expressed metabolites (DEMs) in O. furnacalis under UV-A exposure. Three complementary DNA libraries were constructed from O. furnacalis adult females (CK, UV1h, and UV2h), and 50,106 expressed genes were obtained through Illumina sequencing. Of these, 157 and 637 DEGs were detected in UV1h and UV2h after UV-A exposure for 1 and 2 h, respectively, compared to CK, with 103 and 444 upregulated and 54 and 193 downregulated genes, respectively. Forty four DEGs were detected in UV2h compared to UV1h. Comparative transcriptome analysis between UV-treated and control groups revealed signal transduction, detoxification and stress response, immune defense, and antioxidative system involvement. Metabolomics analysis showed that 181 (UV1h vs. CK), 111 (UV2h vs. CK), and 34 (UV2h vs. UV1h) DEMs were obtained in positive ion mode, while 135 (UV1h vs. CK), 93 (UV2h vs. CK), and 36 (UV2h vs. UV1h) DEMs were obtained in negative ion mode. Moreover, UV-A exposure disturbed amino acid, sugar, and lipid metabolism. These findings provide insight for further studies on how insects protect themselves under UV-A stress.

Using machine learning to predict protein-protein interactions between a zombie ant fungus and its carpenter ant host

“…ecdysone and juvenile hormone), stress response, xenobiotic detoxification processes, and cuticular development (Iga & Kataoka, 2012; Scott & Wen, 2001; Su et al, 2021; Sztal et al, 2012; Huidong Wang et al, 2018; Xing et al, 2021; B. zhong Zhang et al, 2016). For example, we found multiple cytochrome p450 proteins bound uniquely by Ophiocordyceps of the 4C1 subtype that has been implicated in stress response (e.g., social isolation, temperature, UV, or starvation), insecticide/xenobiotic detoxification, and is regulated by hormones including juvenile hormone (Kim Lien et al, 2019; Lu et al, 1999; Scharf et al, 2021; Shen et al, 2021; Su et al, 2021). In turn, we predicted that many of those host cytochrome P450s interacted with fungal cytochrome P450s.…”

“…In insects, cytochrome P450 proteins are often involved in hormone metabolism (e.g. ecdysone and juvenile hormone), stress response, xenobiotic detoxification processes, and cuticular development (Iga & Kataoka, 2012; Scott & Wen, 2001; Su et al, 2021; Sztal et al, 2012; Huidong Wang et al, 2018; Xing et al, 2021; B. zhong Zhang et al, 2016).…”

“…In insects, cytochrome P450 proteins are often involved in hormone metabolism (e.g. ecdysone and juvenile hormone), stress response, xenobiotic detoxification processes, and cuticular development [115][116][117][118][119][120][121]. For example, we found multiple cytochrome p450 proteins bound uniquely by Ophiocordyceps of the 4C1 subtype that has been implicated in stress response (e.g., social isolation, temperature, UV, or starvation) and insecticide/xenobiotic detoxification, and is regulated by hormones including juvenile hormone [117,[122][123][124][125].…”

“…ecdysone and juvenile hormone), stress response, xenobiotic detoxification processes, and cuticular development [115][116][117][118][119][120][121]. For example, we found multiple cytochrome p450 proteins bound uniquely by Ophiocordyceps of the 4C1 subtype that has been implicated in stress response (e.g., social isolation, temperature, UV, or starvation) and insecticide/xenobiotic detoxification, and is regulated by hormones including juvenile hormone [117,[122][123][124][125]. In turn, D-SCRIPT predicted that many of those host cytochrome P450s interacted with fungal cytochrome P450s.…”

Integrated Metabolomics and Transcriptomic Analysis of Hepatopancreas in Different Living Status Macrobrachium nipponense in Response to Hypoxia

“…Therefore, to improve screening the results of high-throughput sequencing, the joint analysis of metabolic and transcriptomic analysis has gradually entered the public field of vision. This method has been widely used in animals by searching for differential metabolites and DEGs, respectively, and then jointly analyzing them to find common differential genes to lock in genes that are related to the purpose of the research [ 15 , 16 , 17 , 18 ].…”