Molecular characterization and expression of CD2 in Nile tilapia (Oreochromis niloticus) in response to Streptococcus agalactiae stimulus

Costimulatory Function of Cd58/Cd2 Interaction in Adaptive Humoral Immunity in a Zebrafish Model

“…Most of these key residues are conserved in zebrafish, such as Glu-46, Trp-49, Lys-50, Lys-55, Glu-58, and Glu-99 of Cd58 and Lys-43, Asp-49, Arg-51, Lys-65, Phe-103, and Gly-107 of Cd2 (Figure 2 C). Interestingly, Tyr-86, an important residue in mammalian CD58 and CD2 interaction, is replaced by Phe-103 in Cd2, which can also be observed in other fish species, such as Oreochromis niloticus ( 4 , 5 , 55 ). Coincidentally, a research in humans has proved that the mutation from Tyr to Phe at site 86 of CD2 has no effect on the interaction between CD58 and CD2.…”

Molecular and functional characterization of peptidoglycan-recognition protein SC2 (PGRP-SC2) from Nile tilapia (Oreochromis niloticus) involved in the immune response to Streptococcus agalactiae

“…The b-actin gene was used as an internal control to normalize the potential variations in RNA loading. The relative expression levels of On-PGRP-SC2 were calculated using Nile tilapia b-actin expression as a reference, and the results were further compared to respective control group expression levels to determine the fold induction [27,28]. The PCR was performed in a 15 mL reaction volume containing 0.5 mL of each primer (10 mM), 1 mL of 10 À1 diluted cDNA, 3 mL PCR-grade water and 10 mL of 2 Â TransStart™ Green qPCR SuperMix (TransGen, China) according to the manufacturer's protocol.…”