Molecular characterization and expression of SiFad1 in the sea urchin (Strongylocentrotus intermedius)

Han, Lingshu; Wang, Heng; Wang, Heng; Zuo, Rantao; Quan, Zijiao; Fan, Zihan; Liu, Quandi; Chang, Yaqing

doi:10.1016/j.gene.2019.04.043

Cited by 15 publications

(9 citation statements)

References 45 publications

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“…Fads A mediates the introduction of unsaturation (double bond) into a fatty acyl chain (Guillou et al 2010), showing Δ5-desaturated activity in which it uses 20: 3n-6 and 20: 4n-3 to transform into ARA and EPA (Kabeya et al 2017). LC-PUFA biosynthesis activity was also reported in other sea urchin’s species such as Strongylocentrotus intermedius (Han et al 2019). The gene expression of Fads A in the gonad is congruent with ARA and the EPA as the most abundant LC-PUFAs in the P. lividus gonad (Sanna et al 2017).…”

Section: Resultssupporting

confidence: 56%

The male and female gonad transcriptome of the edible sea urchin, Paracentrotus lividus: identification of sex-related and lipid biosynthesis genes

Machado

Fernández-Boo

Nande

et al. 2021

Preprint

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Paracentrotus lividus is the most abundant, distributed and desirable echinoid species in Europe. Although, economically important, this species has scarce genomic resources available. Here, we produced and comprehensively characterized the male and female gonad transcriptome of P. lividus. The P. lividus transcriptome assembly has 53,865 transcripts, an N50 transcript length of 1,842 bp and an estimated gene completeness of 97.4% and 95.6% in Eukaryota and Metazoa BUSCO databases, respectively. Differential gene expression analyses yielded a total of 3371 and 3351 up regulated genes in P. lividus male and female gonad tissues, respectively. Additionally, we analysed and validated a catalogue of pivotal transcripts involved in sexual development and determination (206 transcripts) as well as in biosynthesis and storage of lipids (119 transcripts) in male and female specimens. This study provides a valuable transcriptomic resource and will contribute for the future conservation of the species as well as the exploitation in aquaculture settings.HighlightsAssembly of a reference transcriptome of Paracentrotus lividus gonads.Differential gene expression between males and female gonads of Paracentrotus lividus.Identification and validation of pivotal genes involved in biosynthesis and storage of lipids.

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Section: Resultssupporting

confidence: 56%

The male and female gonad transcriptome of the edible sea urchin, Paracentrotus lividus: identification of sex-related and lipid biosynthesis genes

Machado

Fernández-Boo

Nande

et al. 2021

Preprint

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“…Its functional characterization in yeast ( Saccharomyces cerevisiae ) found that two of them showed Δ8 activity, and the other one showed Δ5 activity (Kabeya et al., 2017). Recently, two elongase (Elovl4 and Elovl5) and desaturase (Δ6 and Δ5 desaturases) were cloned from S. intermedius by our research team (Ding et al., 2019; Han et al., 2019). However, it is still unknown about the effects of dietary n‐3 LC‐PUFA on the transcription of rate‐limiting enzymes during the PUFA biosynthesis in adult S. intermedius .…”

Section: Introductionmentioning

confidence: 99%

Effects of dietary n‐3 LC‐PUFA on the growth performance, gonad development, fatty acid profile, transcription of related genes and intestinal microflora in adult sea urchin ( Strongylocentrotus intermedius )

Zhang

Ding

et al. 2020

Aquaculture Research

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A study was conducted to investigate the effects of dietary n‐3 LC‐PUFA on the growth performance, fatty acid profile, transcription of related genes and intestinal microflora in adult sea urchin (Strongylocentrotus intermedius). Kelp (Laminaria japonica) and four formulated feeds with graded levels of n‐3 LC‐PUFA (0%, 0.5%, 0.8% and 1.0% dry diet) were prepared as the experimental diets. Each diet was randomly allocated to ten adult sea urchin individuals. The feeding period lasted for 90 days. Results showed that gonadosomatic index (GSI) of adult S. intermedius fed formulated feeds was significantly higher than that fed kelp (p < 0.05), while weight gain rate (WGR) showed no significance between kelp and feed groups (p > 0.05). Neither WGR nor GSI showed significant differences among the four feed treatments (p > 0.05). Notably, ARA and EPA in the gonads of S. intermedius fed the feeds devoid of n‐3 LC‐PUFA were comparable to that fed the feeds with 0.5% n‐3 LC‐PUFA (p > 0.05). Transcription of Elovl4 and Δ5‐desaturase in the intestine and gonad significantly decreased as dietary n‐3 LC‐PUFA increased (p < 0.05). Proteobacteria and Bacteroidetes (phylum level) and Psychromonas and Sulfurimonas (genus level) were the most abundant bacteria species in the intestine of S. intermedius. The abundance of Sulfurimonas was comparable in the S. intermedius fed kelp and the feed with 0% n‐3 LC‐PUFA, which was significantly higher than that fed the other formulated feeds (p < 0.05). These results indicated that S. intermedius seems to have a low requirement for n‐3 LC‐PUFA to sustain its normal growth and gonad development. The increased transcription of rate‐limiting enzymes (Elovl4 and Δ5‐desaturase) could be responsible for the considerable amounts of EPA and ARA in the gonads of S. intermedius when fed feeds without LC‐PUFA. The intestinal microflora were affected to a certain extent by the die categories and the n‐3 LC‐PUFA in the formulated feeds. It is speculated that some unknown bacteria in the genus of Sulfurimonas could participate in the synthesis of LC‐PUFA.

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“…The analysis was conducted with a LightCycler ® 96 instrument (Roche Life Science, Basel, Switzerland), and gene-specific primers were designed using Primer Premier 5.0 (Supplemental Table S2). The detailed processes of cDNA reverse transcription, gene amplification and internal standardization were decribed in our pervious study 47 . Three independent biological replicates of each gender at each development stage were performed to increase the veracity of the results.…”

Section: Methodsmentioning

confidence: 99%

Integrated metabolomic and transcriptomic analyses identify critical genes in eicosapentaenoic acid biosynthesis and metabolism in the sea urchin Strongylocentrotus intermedius

Wang

Ding

et al. 2020

Sci Rep

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Gonads are the only edible part of the sea urchin and have great potential as a health-promoting food for human consumption. polyunsaturated fatty acids (pUfAs) are important necessary nutrients that determine not only the nutritional value of sea urchins but guarantee their normal growth and reproduction. However, the information on the molecular mechanisms of PUFA biosynthesis and metabolism in this species remains elusive. In this study, we used Strongylocentrotus intermedius as our model species and conducted integrated metabolomic and transcriptomic analyses of potentially critical genes involved in PUFA biosynthesis and metabolism during gonad growth and development, mainly focusing on eicosapentaenoic acid (EPA). We found six differentially accumulated metabolites associated with PUFA in the metabolomic analysis. More differentially expressed genes (DEGs) were related to PUFA in testis than ovary (1823 DEGs in testis and 1499 DEGs in ovary). We verified 12 DEGs by RNA-Seq results and found that Aldh7a1, Ecm3, Fads2, and Hsd17b12 genes had similar expression patterns in EPA concentration during gonad growth and development. In contrast, the other DEGs were downregulated and we inferred that epA or pUfA may be metabolized as energy during certain periods. our metabolic and genetic data will facilitate a better understanding of pUfA regulation networks during gonad growth and development in S. intermedius. The gonads which produce roe are the only edible part of the sea urchins and have great potential to promote benfites to human health 1,2. The sea urchin gonads have high nutritional value and possess several essential nutrients like protein, lipids, polysaccharides, fatty acids, minierlas and vitamins. Polyunsaturated fatty acids (PUFAs) are one of ' the most important and necessary nutrients, with eicosapentaenoic acid (EPA) as the main component among the PUFAs. Many biophysical studies have revealed that PUFAs significantly alter the basic properties of membranes such as acyl chain order, fluidity, elastic compressibility, phase behavior and permeability 3,4 , and play a beneficial role in stabilizing dynamic membrane 5 , membrane organization and cell division 6. In addition, some PUFAs have significant influence on the production of eicosanoids, which involved in the body's inflammatory response and homeostatic processes, neurological disorders, and cardiovascular diseases 7,8. The potential of PUFA in stimulating bone, brain and immune cell development at embryonic through to early phases of the animal's life could significantly improve productivity and welfare 9. In marine invertebrates, PUFAs play a key role in metabolic health and influence various cellular processes, such as serving as a repository for energy reserves or regulating gene expression of metabolic disorders 10. It is believed that PUFAs could be synthesized in marine invertebrates 11 , such as mollusks, in which the biosynthesis of PUFA has been most extensively investigated 12. However, unlike vertebrates, information on

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Molecular characterization and expression of SiFad1 in the sea urchin (Strongylocentrotus intermedius)

Cited by 15 publications

References 45 publications

The male and female gonad transcriptome of the edible sea urchin, Paracentrotus lividus: identification of sex-related and lipid biosynthesis genes

The male and female gonad transcriptome of the edible sea urchin, Paracentrotus lividus: identification of sex-related and lipid biosynthesis genes

Effects of dietary n‐3 LC‐PUFA on the growth performance, gonad development, fatty acid profile, transcription of related genes and intestinal microflora in adult sea urchin ( Strongylocentrotus intermedius )

Integrated metabolomic and transcriptomic analyses identify critical genes in eicosapentaenoic acid biosynthesis and metabolism in the sea urchin Strongylocentrotus intermedius

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