Molecular characterization and expression analysis of the porcine paraoxonase 3 (PON3) gene

Adiponectin gene polymorphisms (T45G and G276T), adiponectin levels and risk for metabolic diseases in an Arab population

“…Screening for additional SNPs in the adiponectin gene may potentially reveal the existence of other such associations within the adiponectin gene and metabolic disease risk in a Saudi population. Other such SNPs linked to T2DM in different ethnic groups might include paraoxonase (PON)3 for dyslipidemia and T2DM (Labrecque et al, 2009); PEA15 gene for diabetes (Wolford et al, 2000), and Npr3 for hypertension (Gilmore, et al, 2001), to name a few. Although we did not find associations with studied SNPs, the findings emphasize that "ethnicity" based differences exist in the associations between adiponectin SNPs and the risk of developing metabolic phenotypes.…”

Effects of supplementation of flax meal and flax oil on mammary gene expression and activity of antioxidant enzymes in mammary tissue, plasma and erythrocytes of dairy cows

“…Extraction of total RNA from mammary tissue and cDNA synthesis was performed as previously described by Labrecque et al (2009). Integrity and purity of extracted RNA was assessed using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies Inc.).…”

Greater milk yield is related to increased DNA and RNA content but not to mRNA abundance of selected genes in sow mammary tissue

“…First strand cDNA was synthesized from 1 mg of total RNA with the Superscript II Reverse Transcriptase using oligo dT(12Á18) primers (Invitrogen Life Technologies) in a total reaction volume of 20 mL. Upon completion of cDNA synthesis, dilutions were performed as previously described (Labrecque et al 2009). …”

“…The PCR amplifications were performed in a 10-mL reaction volume consisting of corresponding concentrations of forward and reverse primers (Table 1), 5 mL of 2) Power SYBRGreen Master Mix (PE Applied BioSystems), 3 mL of 15)diluted cDNA, and 0.05 mL of Uracil N-glycosylase (UNG) AmpErase (PE Applied BioSystems). Cycling conditions were as previously described (Labrecque et al 2009) and amplification, detection, and data analysis were performed with an ABI 7500 Fast Real-Time PCR System (PE Applied Biosystems). Specificity of amplified fragments was verified with the melting curve analysis.…”

“…Amplifications were performed in triplicate and standard curves were established in duplicate for each gene. Standard curves were composed of serial dilutions of complementary DNA pools (Labrecque et al 2009) and were used to obtain the relative quantification of mRNA using the standard curve method as described in the User Bulletin #2 (Applied Biosystems 1997). Relative quantification values were then normalized with the RG.…”