Molecular characterization and expression analysis of a novel C-type lectin (CTL) gene in yellow catfish Pelteobagrus fulvidraco

Tang, Xin; Ye, Xiao-Ting; Wu, Qing; Tang, Ying-Yu; Zhang, Dai-Zhen; Liu, Qiu-Ning; Tang, Boping; Zhu, Haoru

doi:10.1016/j.aqrep.2021.100640

Cited by 4 publications

(3 citation statements)

References 29 publications

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“…The length of the GH gene in P. gigas was 1.176 bp (Lemaire et al, 1994); while in P. hypophthalmus, it was 1.151 bp (Dewi et al, 2009). The GH gene in yellow catfish (Pelteobagrus fulvidraco) has 615 bp ORF translated into a polypeptide amino acid code to 204 (Tang et al, 2021). Tiger shovelnose catfish GH gene has 17 types of amino acids in it.…”

Section: Resultsmentioning

confidence: 99%

ISOLATION AND IDENTIFICATION OF PARTIAL GROWTH HORMONE (GH) mRNA OF TIGER SHOVELNOSE CATFISH Pseudoplatystoma fasciatum (LINNAEUS, 1766)

Hayuningtyas

Kusrini

Abinawanto

2022

Indonesian Aquaculture Journal

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The growth of fish is regulated by growth hormones secreted in a limited amount by its pituitary glands. Tiger shovelnose catfish has a relatively faster growth indicating that some growth hormones suspectedly play roles in the process and could be used in improving other farmed fish species. This study aimed to isolate and identify the growth hormone (GH) mRNA gene in Tiger shovelnose catfish (Pseudoplatystoma fasciatum Linnaeus, 1766). The sample was isolated from the pituitary, the fish measuring 602 g and 43 cm body length; Total RNA was extracted using the Tri Reagent Kit, followed with cDNA synthesis. The success of the extraction was confirmed by quantification and PCR using the β-actin gene as an internal control. GH mRNA gene was isolated by RT-PCR method, with degenerated primers from seven catfish species sequence data in the NCBI gene bank. The single band from GH gene was cloned and sequenced. Total RNA quantification with a concentration of 227 ng/µL and purity of 1.821. The successful isolation of mRNA from the pituitary gland was confirmed by amplifying the β-actin gene generated at 300 bp. This isolation of the GH mRNA gene had a sequence length of 234 bp. Tiger shovelnose catfish GH gene consists of 17 amino acid residues. The GH gene of Tiger shovelnose catfish was close to that of striped catfish (P. hypophthalmus) and Indian catfish (Clarias batrachus) with almost similar homology value of 90.6 %. Partial GH gene from tiger shovelnose catfish can be used a molecular marker in revealing the role of growth hormone on fish development, fish biology, growth gene expression, selective breeding and other mechanisms related to the aquaculture.

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Section: Resultsmentioning

confidence: 99%

ISOLATION AND IDENTIFICATION OF PARTIAL GROWTH HORMONE (GH) mRNA OF TIGER SHOVELNOSE CATFISH Pseudoplatystoma fasciatum (LINNAEUS, 1766)

Hayuningtyas

Kusrini

Abinawanto

2022

Indonesian Aquaculture Journal

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“…One of the most destructive pathogens, causing significant losses, is Aeromonas hydrophila, a Gram-negative bacterium, which is an oxidase-positive and facultative anaerobic. This is an opportunistic aquatic pathogen that produces various virulence factors, including hemolysins, aerolysins, adhesins, enterotoxins, phospholipase, and lipase [4], P. fulvidraco has become increasingly popular as a model fish for studying breeding technology, toxicology, immune response, and lipid metabolism in recent years [5][6][7][8][9]. However, the effect of A. hydrophila on P. fulvidraco has not been reported.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis Revealed Potential Immune-Related Genes of Head Kidney in the Yellow Catfish (Pelteobagrus fulvidraco) Challenged with Aeromonas hydrophila

Jiang,

Lei,

Wang

et al. 2024

Fishes

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Yellow catfish (Pelteobagrus fulvidraco) is an important freshwater fish species in aquaculture. However, as intensive farming has rapidly expanded, infectious diseases caused by various bacteria, such as Aeromonas hydrophila, have also increased. We conducted a transcriptomic analysis of head kidney from P. fulvidraco stimulated by A. hydrophila strain SHOU. A total of 43,249 unigenes with an average length of 1342 bp were obtained following assembly and annotation. By analyzing GO and KEGG enrichment, many differentially expressed genes (DEGs) and pathways related to immunity were identified. Out of the 973 DEGs that were identified, 553 were upregulated and 420 were downregulated. Moreover, KEGG enrichment analysis revealed that the innate immune system, including the TNF signaling pathway, NF-kappa B signaling pathway, and the Toll-like receptor signaling pathway involved in the defense, is activated against infectious response. Real-time quantitative reverse transcription-PCR (qRT-PCR) analysis demonstrated that immune response genes were upregulated in response to A. hydrophila stimulation compared to the control. In conclusion, this study provides valuable insights into the immune defense mechanism of P. fulvidraco and sheds light on the host immune genes involved in the response to bacterial infection.

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“…Lectins are an important component of the innate immune system for PRRs and include one or more carbohydrate recognition domains (CRDs) that can recognize and bind to a carbohydrate on the surface of bacteria, fungi, and viruses [5]. Based on their structure, binding specificity, and calcium dependency, lectins are divided into seven different families, including C-type lectins [6], F-type lectins [7], galectins [8], intelectins (ITLNs) [9], rhamnose-binding lectins [10], I-type lectins [11], and Lily-type lectins [12].…”

Section: Introductionmentioning

confidence: 99%

Molecular Identification and Expression Analysis of an Intelectin Gene in the Yellow Catfish Pelteobagrus fulvidraco (Siluriformes: Bagridae)

Jiang,

Lei,

et al. 2023

Fishes

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Intelectins (ITLNs) are a family of calcium-dependent lectins with carbohydrate-binding capacity, are distributed across various vertebrates, and play an important role in the innate immune response against pathogen infection. The yellow catfish Pelteobagrus fulvidraco (Siluriformes: Bagridae) is an economically important fish in China. The aim of this study was to quantify the gene expression of ITLN in response to pathogen-associated molecular patterns (PAMPs) stimulation. Here, the ITLN gene of P. fulvidraco was characterized and named PfITLN. The full-length cDNA of PfITLN was 1132 bp, including a 5’-untranslated region (UTR) of 140 bp, a 3’-UTR of 110 bp, and an open reading frame (ORF) of 882 bp encoding a polypeptide of 293 amino acids, which contains a signal peptide and two fibrinogen-related domains (FReDs). PfITLN had a molecular weight of 32.39 kDa with a theoretical pI of 5.03. The deduced PfITLN amino acid sequence had 81%, 64%, and 55% homology with Ictalurus furcatus, Danio rerio, and Homo sapiens, respectively. Moreover, the predicted tertiary protein structure of PfITLN was highly similar to that of other animals, and phylogenetic analysis showed that the PfITLN protein was close to those of other Teleostei. Real-time quantitative reverse transcription-PCR (qRT-PCR) analysis showed PfITLN expression in all examined tissues, with the highest abundance seen in the liver, followed by the head kidney, spleen, trunk kidney, and muscle. After PAMP infection with lipopolysaccharide (LPS) and polyriboinosinic polyribocytidylic acid (poly I:C), the expression levels of PfITLN were significantly upregulated at different time points. These results suggested that PfITLN might be involved in innate immunity.

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Molecular characterization and expression analysis of a novel C-type lectin (CTL) gene in yellow catfish Pelteobagrus fulvidraco

Cited by 4 publications

References 29 publications

ISOLATION AND IDENTIFICATION OF PARTIAL GROWTH HORMONE (GH) mRNA OF TIGER SHOVELNOSE CATFISH Pseudoplatystoma fasciatum (LINNAEUS, 1766)

ISOLATION AND IDENTIFICATION OF PARTIAL GROWTH HORMONE (GH) mRNA OF TIGER SHOVELNOSE CATFISH Pseudoplatystoma fasciatum (LINNAEUS, 1766)

Transcriptome Analysis Revealed Potential Immune-Related Genes of Head Kidney in the Yellow Catfish (Pelteobagrus fulvidraco) Challenged with Aeromonas hydrophila

Molecular Identification and Expression Analysis of an Intelectin Gene in the Yellow Catfish Pelteobagrus fulvidraco (Siluriformes: Bagridae)

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