Molecular characterization and expression analysis of the Lrh-1 gene in Chinese Hu sheep

Abstract

ABSTRACT. Liver receptor homologue 1 (Lrh-1) is a member of the nuclear receptor belonging to the second subfamily of the nuclear receptor family 5A (NR5A), also named NR5A2, which is important for lipid homeostasis, embryogenesis, and regulation of aromatics. The present study aimed to understand the sequence of ovine Lrh-1 and the expression traits in reproductive organ tissues. Initially, we cloned Lrh-1 from the liver of Hu sheep through degenerate primer of reverse transcriptionpolymerase chain reaction and rapid amplification of cDNA ends. Characteristic functional domains of DNA binding and ligand binding, conserved among transcription factors of the nuclear receptor superfamily, were identified in Lrh-1 of Hu sheep. The Lrh-1 protein levels in the tissues detected by Western blotting correlated significantly with the transcript levels measured by quantitative real-time polymerase chain reaction (qRT-PCR). To understand the Lrh-1 expression change in the hypothalamus and hypophysis during the estrous cycle, we analyzed the expression pattern of Lrh-1 mRNA and protein by qRT-PCR and Western blotting, respectively. This analysis revealed that Lrh-1 expression in the hypothalamus was highest during the metestrus phase, while the Lrh-1 level was similar during other phases. In the hypophysis, the expression was significantly different during the 4 phases of the estrous cycle but highest during the estrus phase, Characterization of Lrh-1 in ovine significantly correlating with FSH concentration. These results indicate that Lrh-1 expression is correlated with gonadotropic hormone secretion, influencing follicular formation in the ovary.

Molecular characterization, expression, polymorphism of NR5A2 and its relationship with litter size in Hu sheep

“…Mice lacking NR5A2 in granulosa cells were sterile owning to anovulation (Duggavathi et al, 2008). Together, these data suggest that NR5A2 plays an important role in animal ovulation and reproduction (Labelle-Dumains et al, 2007;Duggavathi et al, 2008;Wang et al, 2013).…”

Atividade dos genes relacionados à pluripotência em ovinos

“…Sequência utilizada para sexagem de embriões Bernardi et al, 1996 Os receptores nucleares NR5A2 e NR0B1 são importantes para diversos processos biológicos durante a embriogênese de mamíferos (Wang et al, 2013b;Miao et al, 2016). Em células pluripotentes, NR5A2 é expresso em gametas, embriões e CTEs, além de substituir OCT4 na reprogramação celular por fatores definidos em camundongos (Heng et al, 2010).…”

“…Em células pluripotentes, NR5A2 é expresso em gametas, embriões e CTEs, além de substituir OCT4 na reprogramação celular por fatores definidos em camundongos (Heng et al, 2010). Em ovinos, NR5A2 foi detectado em nível de RNAm e proteína na hipófise, hipotálamo, ovários e ovidutos (Wang et al, 2013b). As células da granulosa de ovinos expressam NR5A2 (Wang et al, 2013b) e NR0B1 (Miao et al, 2016), fato que sugere que este tipo celular seja uma possível alternativa para investigações sobre a função destes genes durante a reprogramação celular em ovinos.…”