Molecular characterization and expression analysis reveal the roles of Cys2/His2 zinc-finger transcription factors during flower development of Brassica rapa subsp. chinensis

Lyu, Tianqi; Liu, Weimiao; Hu, Ziwei; Xiao, Xiang; Liu, Tingting; Xiong, Xingpeng; Cao, Jiashu

doi:10.1007/s11103-019-00935-6

Cited by 16 publications

(11 citation statements)

References 75 publications

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“…The phenotype characterization of the Atzat4 (+/−) mutant showed similar traits to other male-sterile plants [ 16 , 20 , 23 , 24 ]. We observed significative reduced germination and pollen tube elongation in the heterozygous insertional mutant compared with the WT ( Figure 5 C–E).…”

Section: Discussionmentioning

confidence: 76%

“…Several C 2 H 2 -type ZFPs have been described to participate in anther and pollen development in Arabidopsis and other model plant species [ 16 , 20 , 21 , 22 , 23 , 24 ]. AtZAT4 was primarily identified by Mittler et al (2006) [ 27 ] by evaluating the expression of some genes of the AtZAT family under different stress conditions, but so far AtZAT4 from A. thaliana has not been characterized in male reproductive and seeds development.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, AtZAT4 has an EAR repression motif, which has been associated with repressive activity and is found at the C-terminus of the proteins ( Figure S1 ) [ 38 ]. Several studies have shown that C 2 H 2 -ZFPs containing QALGGH and EAR transcriptional repression motifs participate in the development of plant reproductive organs [ 16 , 19 , 22 , 24 ]. In addition, according to in silico prediction, AtZAT4 has an NLS ( Figure S1 ).…”

Section: Discussionmentioning

confidence: 99%

“…In Brassica campestris , the C 2 H 2 -ZFP BcMF20 has been proposed to be involved in the development of tapetum [ 23 ]. Lastly, BrZFP38 from B. rapa is involved in the late development of pollen grain as well as pollination [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

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AtZAT4, a C2H2-Type Zinc Finger Transcription Factor from Arabidopsis thaliana, Is Involved in Pollen and Seed Development

Puentes-Romero

González

González-Villanueva

et al. 2022

Plants

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Pollen plays an essential role in plant fertility by delivering the male gametes to the embryo sac before double fertilization. In several plant species, including Arabidopsis, C2H2-type zinc-finger transcription factors (TFs) have been involved in different stages of pollen development and maturation. ZINC FINGER of Arabidopsis thaliana 4 (AtZAT4) is homologous to such TFs and subcellular localization analysis has revealed that AtZAT4 is located in the nucleus. Moreover, analysis of AtZAT4 expression revealed strong levels of it in flowers and siliques, suggesting a role of the encoded protein in the regulation of genes that are associated with reproductive development. We characterized a T-DNA insertional heterozygous mutant Atzat4 (+/−). The relative gene expression analysis of Atzat4 (+/−) showed significant transcript reductions in flowers and siliques. Furthermore, the Atzat4 (+/−) phenotypic characterization revealed defects in the male germline, showing a reduction in pollen tube germination and elongation. Atzat4 (+/−) presented reduced fertility, characterized by a smaller silique size compared to the wild type (WT), and a lower number of seeds per silique. Additionally, seeds displayed lower viability and germination. Altogether, our data suggest a role for AtZAT4 in fertilization and seed viability, through the regulation of gene expression associated with reproductive development.

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Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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AtZAT4, a C2H2-Type Zinc Finger Transcription Factor from Arabidopsis thaliana, Is Involved in Pollen and Seed Development

Puentes-Romero

González

González-Villanueva

et al. 2022

Plants

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“…The role of WRKY and zinc finger-containing transcription factors in abiotic stress response has been very well documented in various plants ( Khan et al, 2018 ; Yoon et al, 2020 ). Both of these are classically large TF families comprising many members, which makes their role in plant development more diverse and not limited to stress response ( Li M. et al, 2020 ; Lyu et al, 2020 ; Wani et al, 2021 ). More recently, the focus has shifted to smaller families of transcription factors, which have a more direct role in abiotic stress response in plants.…”

Section: Discussionmentioning

confidence: 99%

De novo Whole-Genome Assembly of Moringa oleifera Helps Identify Genes Regulating Drought Stress Tolerance

et al. 2021

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Abiotic stresses, especially drought stress, are responsible for heavy losses in productivity, which in turn poses an imminent threat for future food security. Understanding plants’ response to abiotic stress at the molecular level is crucially important for mitigating the impacts of climate change. Moringa oleifera is an important multipurpose plant with medicinal and nutritional properties and with an ability to grow in low water conditions, which makes the species an ideal candidate to study the regulatory mechanisms that modulate drought tolerance and its possible use in agroforestry system. In the present communication, we report whole-genome sequencing (WGS) of this species and assemble about 90% of the genome of M. oleifera var. Bhagya into 915 contigs with a N50 value of 4.7 Mb and predicted 32,062 putative protein-coding genes. After annotating the genome, we have chosen to study the heat shock transcription factor (HSF) family of genes to analyze their role in drought tolerance in M. oleifera. We predicted a total of 21 HSFs in the M. oleifera genome and carried out phylogenetic analyses, motif identification, analysis of gene duplication events, and differential expression of the HSF-coding genes in M. oleifera. Our analysis reveals that members of the HSF family have an important role in the plant’s response to abiotic stress and are viable candidates for further characterization.

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Plant biotechnologies for processing raw products in phytomedicines

Butnariu

Buţu

2021

Phytomedicine

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Molecular characterization and expression analysis reveal the roles of Cys2/His2 zinc-finger transcription factors during flower development of Brassica rapa subsp. chinensis

Cited by 16 publications

References 75 publications

AtZAT4, a C2H2-Type Zinc Finger Transcription Factor from Arabidopsis thaliana, Is Involved in Pollen and Seed Development

AtZAT4, a C2H2-Type Zinc Finger Transcription Factor from Arabidopsis thaliana, Is Involved in Pollen and Seed Development

De novo Whole-Genome Assembly of Moringa oleifera Helps Identify Genes Regulating Drought Stress Tolerance

Plant biotechnologies for processing raw products in phytomedicines

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