Molecular characterization and expression analysis of caveolin-1 in pig tissues

Wang, Chong; Mei, Yingjie; Li, Li; Mo, Delin; Li, Jiaqi; Zhang, Hao; Tian, Xingguo; Chen, Yaosheng

doi:10.1007/s11427-008-0082-0

Cited by 8 publications

(8 citation statements)

References 33 publications

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“…This sequence of CSD exactly corresponds to the sequence of Sus scrofa caveolin-1 at this position (Wang et al 2008). A scrambled peptide was used as a control (Calbiochem/Merck, Darmstadt, Germany).…”

Section: Methodsmentioning

confidence: 84%

Effect of Cavtratin, a Caveolin-1 Scaffolding Domain Peptide, on Oligodendroglial Signaling Cascades

2011

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Caveolin and caveolin containing rafts are involved in the signaling of growth factors in various cell types. Previous reports of our lab indicated a co-localization of caveolin and the high affinity nerve growth factor (NGF) receptor tyrosine kinase A (TrkA). Mutual effects have been observed among which a caveolin-1 knock-down resulted in an impairment of the NGF signaling cascade rather than in an increase of activity as expected from other growth factor reports. On the other hand, an over-expression of caveolin-1 impaired the NGF stimulated activity of p42/44 mitogen activated protein kinases (MAPK). In this study, we used a caveolin-1 scaffolding domain (CSD) peptide (cavtratin) of which an inhibitory effect on growth factor receptors was reported. Our data showed that cavtratin suppresses the NGF-induced phosphorylation of TrkA as well as the activation of MAPK in porcine oligodendrocytes significantly.

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Section: Methodsmentioning

confidence: 84%

Effect of Cavtratin, a Caveolin-1 Scaffolding Domain Peptide, on Oligodendroglial Signaling Cascades

2011

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“…Os dados revelam imunomarcação intensa para as proteínas cav -1 e -2 localizadas no placentônio, visto que essas proteínas são importantes para manter a aparência morfológica da cavéola e estão envolvidos no metabolismo lipídico, crescimento e apoptose celular (Wang et al 2008), eventos importantíssimos para o ótimo desenvolvimento dos conceptos e saúde materna, e o entendimento desses mecanismos nos auxiliam a entender as razões de tantas perdas gestacionais e anormalidades placentárias, tanto em gestações naturais, como em manipuladas em laboratório. Sendo assim, o estudo das proteínas caveolinas -1 e -2 em conceptos bovinos clonados transgênicos auxíliará para futuros estudos relacionados com desenvolvimento placentário e transporte via placenta em mamíferos.…”

Section: Discussionunclassified

Caracterização das proteínas caveolinas -1 e -2 na placenta de conceptos bovinos clonados transgênicos

et al. 2015

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RESUMO.-A utilização da transgenia com a proteína fluorescente verde (GFP) como marcador de células de origem fetal nas placentas de clones bovinos servirá de modelo inédito para estudo morfofisiológico e imunológico da interação materno-fetal, visto que possibilitará o seu mapeamento, diferenciando as células fetais das maternas. TalCaracterização das proteínas caveolinas -1 e -2 na placenta de conceptos bovinos clonados transgênicos The transgenic application of green fluorescent protein (GFP) as fetal cell marker on cattle cloned placenta could provide an exclusive model for studying the morphologic and immunologic maternal-fetal interactions, providing information about its mapping, distinguishing the fetal from maternal cells. This model will have direct application, mainly because these animals present problems during its development. With this model's support, we intend to verify the substances transport between mother and fetus during endocytosis, through the immunolocalization of protein named caveolae. For these, we used 06 cloned bovine and 30 cattle samples of artificial insemination (AI) with 90 days of pregnancy, which had been their development interrupted by humanitarian slaughter of the recipient and recovery of the pregnant uterus. We collected the placentome and the chorion. A part of the samples was cut and fixed, by immersion, on a solution containing 4% of parafomaldehyde or 10% of formaldehyde on a sodium phosphate buffer (PBS), at 0,1M pH 7.4, Zamboni solution (4% of paraformaldehyde, 15% of picric acid, on sodium phosphate buffer 0,1M pH 7.4), metacarn (60% of metanol, 30% of chloroform, and 10% glacial acetic acid), for morphologic and immunohistochemistry verification for caveolinas proteins -1 and -2 (CAV -1 and CAV-2). The caveolins -1 were found in fetal and maternal villi, but its strongest staining was observed in the endometrial stroma. The caveolins -2 had positive staining in trophoblast and chorioallantoic membrane, and specifically in giant trophoblastic binucleated cell. Therefore the results were compared between cloned cattle and from AI or natural mating, for assisting on detection of the reason of many placental alterations, embryonic losses, spontaneous abortion, post-natal mortality and large offspring syndrome on laboratory-manipulated animals. The result suggests that the proteins caveolins -1 and -2 (CAV-1 and CAV-2) are part of the caveolae composition and important structures related to the molecule transfer to the fetus, nourish it through endocytosis and pinocytosis.INDEX TERMS: Caveolin-1 and -2 proteins, caveolae, placentation, immunolocalization, cattle.

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“…As a major fatty acid binding protein in adipocytes, CAV-1 moves from the plasma membrane to lipid droplets under the action of free fatty acids [68,69]. Compared with Large YK pigs, CAV-1 showed a signi cantly higher expressional level in the backfat(P < 0.01)and longissimus dorsi muscle P < 0.05 in LT [70]. CAV-1-de cient mice showed a lean body phenotype, insulin resistance, and hypertriglyceridemia with adipocyte abnormalities [71].…”

Section: Discussionmentioning

confidence: 97%

LncRNA IMFlnc1 promotes porcine intramuscular adipocyte differentiation by sponging miR-199a-5p to up-regulate CAV-1

Wang

Chen

et al. 2020

Preprint

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Background Local Chinese local pig breeds have better meat quality, such as thinner muscle fiber and higher intramuscular-fat (IMF) content. However, its molecular regulation mechanism has not been discussed in-depth. Studies indicated that long non coding RNAs (lncRNAs) participate in the regulation of muscle and fat development. The expressional differences of lncRNAs in the longissimus dorsi (LD) muscle were identified between Huainan pigs (local Chinese pigs, fat-type, HN) and Large White pigs (lean-type, LW) at 38, 58, and 78 days post conception (dpc).Results In total, 2131 novel lncRNAs were identified in 18 samples, and 291, 305, and 683 differentially expressed lncRNAs (DELs) were found between these two breeds at three stages, respectively. GO and KEGG analysis of the DEL co expressed mRNAs showed that muscle development and energy metabolism were more active at 58 dpc in HN, but at 78 dpc in LW pigs. Muscle cell differentiation and myofibril assembly might contribute to earlier myogenesis and primary-muscle-fiber assembly in HN, and cell proliferation, insulin, and the mitogen-activated protein kinase (MAPK) pathway might contribute to longer proliferation in LW pigs. The PI3K/Akt and cAMP pathways were associated with higher IMF deposition in HN. IMFlnc1 was selected for functional verification, and results indicated that it regulated the expressional level of caveolin-1 (CAV-1) as a competing endogenous RNA (ceRNA) for miR-199a-5p.Conclusion Our data contributed to understanding lncRNAs in porcine-muscle development and IMF deposition, and provided valuable information for improving pig-meat quality.

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Molecular characterization and expression analysis of caveolin-1 in pig tissues

Cited by 8 publications

References 33 publications

Effect of Cavtratin, a Caveolin-1 Scaffolding Domain Peptide, on Oligodendroglial Signaling Cascades

Effect of Cavtratin, a Caveolin-1 Scaffolding Domain Peptide, on Oligodendroglial Signaling Cascades

Caracterização das proteínas caveolinas -1 e -2 na placenta de conceptos bovinos clonados transgênicos

LncRNA IMFlnc1 promotes porcine intramuscular adipocyte differentiation by sponging miR-199a-5p to up-regulate CAV-1

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