Molecular characterization and distribution of Bacillus thuringiensis cry1 genes from Brazilian strains effective against the fall armyworm, Spodoptera frugiperda

Valicente, Fernando Hercos; Picoli, Edgard Augusto de Toledo; Vasconcelos, M. J. V. de; Carneiro, N. P.; Carneiro, A. A.; Guimarães, C. T.; Lana, U. G. de P.

doi:10.1016/j.biocontrol.2010.02.003

Cited by 32 publications

(31 citation statements)

References 22 publications

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“…Using specific cry primers, the cry1 gene was detected in all isolates, the cry1Ac gene was the most present with 45.9% (17 isolates), followed by the cry1Ab gene with 32.4% (12 isolates), cry1Aa with 18.9% (9 isolates), cry1D with 5.4% (2 isolates), cry2 with 10,8% (4 isolates) and cry2Ab with 5,4% (2 isolates) ( Figure 2). Different Bt screening programs worldwide have identified the cry1 genes as the most common crytype gene (BRAVO et al, 1998;URIBE et al, 2003;BERÓN;SALERMO, 2006;SU et al, 2007;GAO et al, 2008;SANTOS et al, 2009;VALICENTE et al, 2010).…”

Section: Identification Of Cry Genesmentioning

confidence: 99%

“…Valicente et al (2010) suggested that the toxicity of different Brazilian B. thuringiensis isolates to S. frugiperda was related to their cry1 gene content. The cry genes frequencies and profiles differ among worldwide Bt collections based on region, moreover, they reported that the genes most frequently found in Minas Gerais, Amazonas, Paraná and São Paulo State, Brazil were cry1D, cry1G, cry1B and cry1E, respectively.…”

Section: Identification Of Cry Genesmentioning

confidence: 99%

“…The biological control of S. frugiperda has been attempted using various microorganisms. Bacillus thuringiensis (BERLINER, 1911) (Bt) produces an array of Cry proteins with are potentially toxic to S. frugiperda (SANTOS et al, 2009;VALICENTE et al, 2010). The interest in this microorganism stems from its potential as an economical, effective, speciesspecific and environmentally safe pesticide (ARRIETA; ESPINOSA, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Selection and characterization of Bacillus thuringiensis isolates with a high insecticidal activity against Spodoptera frugiperda (Lepidoptera: Noctuidae)

et al. 2016

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Spodoptera frugiperda (SMITH, 1797) (Lepidoptera: Noctuidae) affects diverse crops of great economic interest, for instance, it can cause severe yield losses in maize, rice and sorghum. In this study, a selection and characterization of Bacillus thuringiensis (BERLINER, 1911) isolates with a high insecticidal activity against S. frugiperda was performed. Fifty-two crystal-forming B. thuringiensis isolates that were identified from 3384 Bacillus-like colonies were examined and screened by PCR for the presence cry genes (cry1, cry1Aa, cry1Ab, cry1Ac, cry1D, cry2 and cry2Ab). Four isolates that showed high toxicity towards S. frugiperda were shown to harbor cry2 genes. The crystals were analyzed by electron microscopy and showed bipyramidal and cuboidal shapes. Furthermore, these four isolates had lethal concentration (LC 50 ) values of 44.5 ng/cm 2 (SUFT01), 74.0 ng/cm 2 (SUFT02), 89.0 ng/cm 2 (SUFT03) and 108 ng/cm 2 (SUFT 04) to neonate S. frugiperda larvae. An ultrastructural analysis of midgut cells from S. frugiperda incubated with the SUFT01 spore-crystal complex showed disruptions in cellular integrity and in the microvilli of the midgut columnar cells. The isolates characterized in this work are good candidates for the control of S. frugiperda, and could be used for the formulation of new bioinsecticides.

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Section: Identification Of Cry Genesmentioning

confidence: 99%

Section: Identification Of Cry Genesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Selection and characterization of Bacillus thuringiensis isolates with a high insecticidal activity against Spodoptera frugiperda (Lepidoptera: Noctuidae)

et al. 2016

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“…Preventive and curative pest control measures must be undertaken in crop production systems to reduce losses inflicted by insect pests to allow for a sustainable crop production. The strategies available for controlling S. frugiperda still rely on the heavily use of insecticides in many areas of the world, although new technologies are becoming increasingly available (Valicente et al 2010, Kos et al 2009. The use of insecticides is known to cause a number of undesired effects to the nontarget species and to the environment, and to speed the process of development of insecticide resistance in insects (Hardstone & Scott 2010).…”

Section: Introductionmentioning

confidence: 99%

Biochemical Analysis of a Castor Bean Leaf Extract and its Insecticidal Effects Against Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae)

Rossi¹,

Santos

Carvalho

et al. 2012

Neotrop Entomol

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“…To produce crystal proteins, B. thuringiensis strains were cultured on Luria Bertani (LB) solid medium enriched with salts according to the method described by Valicente et al (2010), for 24 h at 30°C. From the 24 h culture, the strains were transferred in 50 ml nutrient broth and incubated at 30°C for 72 h with continuous stirring at 200 rpm (Ammouneh et al, 2011).…”

Section: Production Extraction and Separation Of Crystalline Proteinsmentioning

confidence: 99%

Molecular characterization and insecticidal activities of Malian native crystalliferous Bacillus thuringiensis

Fane¹,

Kassogue²,

Traoré³

et al. 2017

Afr. J. Biotechnol.

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The objective of this work was to select the most active Bacillus thuringiensis (Bt) isolated from agricultural soils of Mali through the molecular characterization and the determination of insecticidal activities of the protein crystals, produced by these native isolates. Crystal proteins were extracted from B. thuringiensis culture, and characterized using the SDS-PAGE techniques. Their insecticidal activities were tested using third-instar larvae of Helicoverpa armigera in bioassay tests. The results showed that, of 62 B. thuringiensis treated, 52 isolates showed fragments varying between 10 and 140 kDa on 12% polyacrylamide gel. Cry1 and Cry2 protein crystals were recognized to be effective against Lepidoptera's larvae, which were found in 21% of the tested isolates. In addition to these two expected crystal protein weights, other molecular weights were observed at different proportions, suggesting the presence of other cry genes in the local B. thuringiensis isolates. Four native B. thuringiensis isolates were able to kill 95 to 100% of H. armigera 3rd-instar larvae. Only one native of B. thuringiensis isolate was able to kill 100% of the H. armigera larvae. This is the first study for molecular characterization of Malian native B. thuringiensis isolates, showing the efficacy of the native B. thuringiensis against an important agricultural insect pest.

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Molecular characterization and distribution of Bacillus thuringiensis cry1 genes from Brazilian strains effective against the fall armyworm, Spodoptera frugiperda

Cited by 32 publications

References 22 publications

Selection and characterization of Bacillus thuringiensis isolates with a high insecticidal activity against Spodoptera frugiperda (Lepidoptera: Noctuidae)

Selection and characterization of Bacillus thuringiensis isolates with a high insecticidal activity against Spodoptera frugiperda (Lepidoptera: Noctuidae)

Biochemical Analysis of a Castor Bean Leaf Extract and its Insecticidal Effects Against Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae)

Molecular characterization and insecticidal activities of Malian native crystalliferous Bacillus thuringiensis

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