Biochemical Analysis of a Castor Bean Leaf Extract and its Insecticidal Effects Against Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae)

Rossi, Gabriel Augusto Marques; Santos, Custódio Donizete dos; Carvalho, Geraldo Andrade; Alves, Dejane Santos; Pereira, Luciana Lopes Silva

doi:10.1007/s13744-012-0078-0

Cited by 22 publications

(17 citation statements)

References 32 publications

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“…Brahim et al [25] studied the toxicity of aqueous extracts of the plant against mosquito larvae of Culex pipiens, Aedes caspius, Culiseta longiareolata and Anopheles maculipennis (Diptera: Culicidae). The leaf extract of R. communis has been shown to posses insecticidal properties against insect pests like Spodoptera frugiperda [26]; Callosobruchus chinensis [27] and Cosmopolites sordidus (Coleoptera: Curculionidae) [28]. Several studies have reported the toxic effects of various plant extracts in control of fly populations of M. domestica [29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

Toxicity of Leaf Extracts of Ricinus communis L. (Euphorbiaceace) Against the Third Instar Larvae of Musca domestica L. (Diptera: Muscidae)

Singh¹

2016

AJBIO

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The housefly, Musca domestica is a ubiquitous insect that has potential to spread wide variety of pathogens to humans and livestock animals leading to diseases and huge economic losses in developing countries. Flies have resisted human attempts to control them since antiquity and the problem of fly resistance to synthetic insecticides had resulted in need to develop biopesticides as an alternative management tool. Plant product are the most promising sources and under extensive trials for their insecticidal activity against various insect species. This study evaluated the efficacy of crude extracts of the castor plant Ricinus communis against Musca domestica by using dipping and thin film technique. The laboratory bioassay in both techniques resulted in considerable larval and pupal mortalities indicating toxicity of plant extract against the fly. Besides, the larval mortalities the extracts induced developmental aberrations such as reduced pupations and non emergence of adults.The results indicate that the plant extracts contain certain active principles which interfere with the hormonal control of development affecting the life cycle of the fly. It can be concluded that crude extract of R. communis can be effectively used as in controlling fly populations of M. domestica as the safer, ecofriendly and economic alternative to synthetic insecticidal agents.

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Section: Introductionmentioning

confidence: 99%

Toxicity of Leaf Extracts of Ricinus communis L. (Euphorbiaceace) Against the Third Instar Larvae of Musca domestica L. (Diptera: Muscidae)

Singh¹

2016

AJBIO

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“…Previous works performed with castor oil leaves suggested that trypsin inhibitor is a nonproteic organic molecule (Rossi et al, 2012) differing from other trypsin inhibitors described in literature. Precipitations with acetone and saturated ammonium solution, high temperature exposition and inhibition assays in the presence of de β-mercapto-ethanol were performed demonstrating the molecule non-proteic characteristic and suggesting different ways to extract and purify this trypsin inhibitor.…”

Section: Discussionmentioning

confidence: 76%

Toxic effects of Ricinus communis non-protein trypsin inhibitor on Spodoptera frugiperda (J. E. Smith) (Lepidoptera:Noctuidae)

Carvalho¹,

Santos²,

Alves³

et al. 2015

Afr. J. Biotechnol.

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In the study reported herein, we aimed to isolate a trypsin inhibitor from Ricinus communis leaves through chromatographic and spectrometric techniques and evaluate its toxic effects on the development of Spodoptera frugiperda larvae. Plant extracts were submitted to fractionation in adsorption column. The fraction 10, which showed the highest inhibitory activity, were incorporated into an artificial diet at the concentrations of 0, 0.06, 0.12, 0.25 and 0.5%, and offered to S. frugiperda larvae. Fresh weight of larvae, food consumed and weight of eliminated faeces were registered. Based on these parameters the following nutritional index were calculated: Relative Consumption Rate (RCR), Relative Metabolic Rate (RMR), Relative Growth Rate (RGR), Approximated Digestibility (AD), Efficiency of Ingested Food Conversion (EIC), Efficiency of Digested Food Conversion (EDC) and the Metabolic Cost (100 -EDC). The inhibitor at 0.5% concentration was deleterious to S. frugiperda, extending the larval stage in 11 days, with higher RCR and ECD, and lower RGR, ECI and ECD. Therefore, the trypsin inhibitor from leaves of R. communis affected the S. frugiperda larval development, being promising in studies of alternative and sustainable control methods for lepidopteran pest species.

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“…In general, percent mortality of the larvae due to insecticide application on castor had been observed to be decreased when the larvae were shifted from cotton (Figure 4e). This could be due to the fact that castor is grown as a perennial crop and S. exigua is habitual to consume it as one of the favorable hosts (Ahmad & Iqbal, 2009; Ahmad et al., 2007; Ishtiaq & Saleem, 2011; Rossi, Santos, Carvalho, Alves, & Pereira, 2012). Similarly, when the larvae sourced from all five hosts were switched to spinach, percent mortality due to all insecticides except cypermethrin decreased.…”

Section: Discussionmentioning

confidence: 99%

Switching among natal and auxiliary hosts increases vulnerability of Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae) to insecticides

Saeed¹,

Saeed

Ahmad

2017

Ecology and Evolution

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The role of insecticidal application and host plant resistance in managing Spodoptera exigua has been well documented, but the effect of different host plants, on which the pest cycles its population in the field, has seldom been investigated. Therefore, we have studied the vulnerability of S. exigua against commonly used insecticides (cypermethrin, chlorpyrifos, lufenuron, and emamectin benzoate) with different mode of actions when it switches its generations from natal to auxiliary hosts and vice versa. Different field populations being established on different host plants including castor, cauliflower, cotton, okra, and spinach were collected and reared in the laboratory before insecticidal bioassays. The role of larval diet and host plant switching on their response to tolerate applied insecticides was studied using leaf‐dip bioassay methods. Host switching demonstrated a significant role in altering the vulnerability of S. exigua populations to tested insecticides. Spodoptera exigua sourced from castor, when switched host to okra and spinach, exhibited 50% higher mortality when treated with emamectin benzoate. This trend in mortality was consistent upon complete host switch cycle (natal—auxiliary—natal host). However, the highest increase (92%) in vulnerability was recorded when the larvae were shifted to spinach from cotton. In general, chlorpyrifos and lufenuron had highest efficacies in terms of larval mortality. The findings of present studies provide insights to a better understanding the behavior of polyphagous pests and the role of different host plants in altering the susceptibility of these pests against applied insecticides. Ultimately the results warrant that due consideration should be given to cropping patterns and time of host switching by pest population during planning and executing chemical control.

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Biochemical Analysis of a Castor Bean Leaf Extract and its Insecticidal Effects Against Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae)

Abstract: Keywords Euphorbiaceae, fall armyworm, pest control, plant extract, Ricinus communis

Cited by 22 publications

References 32 publications

Toxicity of Leaf Extracts of <i>Ricinus communis</i> L. (Euphorbiaceace) Against the Third Instar Larvae of <i>Musca domestica</i> L. (Diptera: Muscidae)

Toxicity of Leaf Extracts of <i>Ricinus communis</i> L. (Euphorbiaceace) Against the Third Instar Larvae of <i>Musca domestica</i> L. (Diptera: Muscidae)

Toxic effects of Ricinus communis non-protein trypsin inhibitor on Spodoptera frugiperda (J. E. Smith) (Lepidoptera:Noctuidae)

Switching among natal and auxiliary hosts increases vulnerability of Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae) to insecticides

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Biochemical Analysis of a Castor Bean Leaf Extract and its Insecticidal Effects Against Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae)

Abstract: Keywords Euphorbiaceae, fall armyworm, pest control, plant extract, Ricinus communis

Cited by 22 publications

References 32 publications

Toxicity of Leaf Extracts of &lt;i&gt;Ricinus communis&lt;/i&gt; L. (Euphorbiaceace) Against the Third Instar Larvae of &lt;i&gt;Musca domestica&lt;/i&gt; L. (Diptera: Muscidae)

Toxicity of Leaf Extracts of &lt;i&gt;Ricinus communis&lt;/i&gt; L. (Euphorbiaceace) Against the Third Instar Larvae of &lt;i&gt;Musca domestica&lt;/i&gt; L. (Diptera: Muscidae)

Toxic effects of Ricinus communis non-protein trypsin inhibitor on Spodoptera frugiperda (J. E. Smith) (Lepidoptera:Noctuidae)

Switching among natal and auxiliary hosts increases vulnerability of Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae) to insecticides

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Toxicity of Leaf Extracts of <i>Ricinus communis</i> L. (Euphorbiaceace) Against the Third Instar Larvae of <i>Musca domestica</i> L. (Diptera: Muscidae)

Toxicity of Leaf Extracts of <i>Ricinus communis</i> L. (Euphorbiaceace) Against the Third Instar Larvae of <i>Musca domestica</i> L. (Diptera: Muscidae)