Molecular characterization and association analysis of porcine <i>CA3</i>

Abstract

Carbonic anhydrase 3 (CA3) is a member of the carbonic anhydrase family, which plays an important role in various cell processes. In this paper, molecular characterization revealed that CA3 genomic DNA consists of seven exons and six introns, spans about 10.5 kb and maps to porcine chromosome 4q11→q14. Results of expression profiles showed that the expression levels of CA3 increased in skeletal muscles from prenatal 33- to 65-day-old Chinese Tongcheng pigs. These levels subsequently decreased to a steady state in prenatal 90-day-old, postnatal 2-day-old, postnatal 28-day-old, and pregnant 65-day-old pigs. The expression patterns of Chinese Tongcheng pig embryos were different from that of Landrace pig embryos. CA3 was expressed at higher levels in skeletal muscle and liver than in kidney, lung, stomach, intestine, and brain, but was not detected in heart and spleen. Statistical analysis showed the CA3 gene polymorphism was different between Chinese indigenous and introduced commercial western pig breeds, and was associated with intramuscular fat content and percentage of ham of pigs.

Expression pattern and polymorphism of three microsatellite markers in the porcineCA3gene

“…In our study, the porcine CA3 gene was also differentially expressed in the skeletal muscle of Yorkshire and Meishan pig breeds. The results of expression profiles showed that the expression levels of CA3 increased in skeletal muscles from prenatal 33-to 65-day-old Chinese Tongcheng pigs and subsequently decreased to a steady state in prenatal 90-day-old, postnatal 2-day-old, postnatal 28-dayold, and pregnant 65-day-old pigs [11]. Therefore, our results are supported by these former reports.…”

“…In our study, we isolated the whole genomic DNA sequence of the porcine CA3 gene and found that its structural features were identical to those reported by Wang et al [11]. Moreover, we analysed the polymorphism of microsatellites present in the whole genomic DNA sequence of CA3.…”

“…Liu et al [7] isolated the porcine CA3 gene by mRNA differential display in the longissimus dorsi muscle tissues from a Landrace × Large White cross-combination. Wang et al [11] analysed temporal and spatial expression differences of the CA3 gene in the pig and reported that the CA3 protein is associated with intramuscular fat content and ham percentage.…”

Mass transfer of CO2 in MAP systems: Advances for non-respiring foods

“…In tissues, the CO 2 transfer could also be facilitated by the presence of an intrinsic mechanism such as the enzymatic activity of carbonic anhydrase (Nomenclature of Enzyme Classification: EC 4.2.1.1. ), present in cows (Poulsen et al, 2005), pigs (Wang et al, 2006), chickens (Yoshihara et al, 1984), fish (Tohse et al, 2006) and others non-processed fresh foods…”

The normalisation ofCAPNgene expression inM. pectoralis superficialisin broiler lines differing in growth rate and their relationship to breast muscle tenderness

“…Kwon et al (2009) examined over two thousand reference genes to find the most stable genes between different human tissues and only 13 of all investigated genes had a low coefficient of variation (CV) and could be used as endogenous controls. Genes commonly used as endogenous controls include ACTB and GAPDH (Wang et al, 2006), but some authors have observed that they obtain poor stability results (Glare et al, 2002). Therefore, the initial aim of this study was to find the most stable endogenous controls for the analysis of gene expression in chicken muscle tissue.…”