Expression pattern and polymorphism of three microsatellite markers in the porcineCA3gene

Abstract

-Carbonic anhydrase III (CA3) is an abundant muscle protein characteristic of adult type-1, slow-twitch, muscle fibres. In order to further understand the functions of the porcine CA3 protein in muscle, the temporal and spatial distributions of its gene product were analysed and the association between the presence of specific polymorphisms and carcass traits in the pig was also examined. Real-time PCR revealed that the CA3 mRNA expression showed no differences with age in skeletal muscles from Yorkshire pigs at postnatal day-1, month-2, and month-4. We provide the first evidence that CA3 is differentially expressed in the skeletal muscle of Yorkshire and Meishan pig breeds. In addition, the whole pig genomic DNA sequence of CA3 was investigated and shown to contain seven exons and six introns. Comparative sequencing of the gene from three pig breeds revealed the existence of microsatellite SJ160 in intron 5 and microsatellite SJ158 and a novel microsatellite marker that includes a tandem repeat of (TC) n in intron 4. We also determined the allele number and frequencies of the three loci in seven pig breeds and found that they are low polymorphic microsatellite markers. Statistical analysis showed that the CA3 microsatellite polymorphism was associated with dressing percentage, internal fat rate, carcass length, rib number and backfat thickness in the pig.pig / carbonic anhydrase III / expression pattern / microsatellite polymorphism

The liver transcriptome of two full-sibling Songliao black pigs with extreme differences in backfat thickness

“…Previously, the expression levels of CA3 , SERPINA6 , GATM , GSTA2 , and ALAS1 were also found to be significantly different between the two groups [9]. CA3 is associated with the internal fat rate and backfat thickness ofthe pig [20], while SERPINA6 within the quantitative trait loci is associated with cortisol levels, fat, and muscle content, so can be considered a key regulator of obesity susceptibility [21]. These findings indicate that CA3 and SERPINA6 are strongly associated with fatty traits.…”