Molecular characterization and antimicrobial susceptibility of Listeria monocytogenes isolated from foods and humans

Su, Xudong; Zhang, Jianmin; Shi, Weimin; Yang, Xiaowei; Li, Yong; Pan, Hongyu; Kuang, Dai; Xu, Xuebin; Shi, Xianming; Meng, Jianghong

doi:10.1016/j.foodcont.2016.04.020

Cited by 34 publications

(21 citation statements)

References 47 publications

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“…Despite that, ANOVA test reported that our extremely variable results are significantly connected (P<0.01) to the strains tested; thus, to different cellular answers, when occurring injuring factors. It is well known that sublethal stressors can induce L. monocytogenes adaptation and develop of specific resistance against several substances such as antibiotics, bacteriophages and disinfectants (Fister et al , 2016; Kovacevic et al , 2016; Su et al , 2016). L. monocytogenes population is characterized by different stress robustness parameters that may represent an advantage in unfavorable condition.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the antibacterial activity of bergamot essential oils on different Listeria monocytogenes strains

et al. 2016

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Essential oils are aromatic and volatile substances extracted from plants and characterized by antimicrobial activity. The aim of the present study was to evaluate the antibacterial activity (agar disc-diffusion method) of seven different bergamot essential oils (BEOs) on eight Listeria monocytogenes strains. Minimal inhibitory concentration (MIC) of most efficient BEOs was estimated. Extremely variable results for agar disc-diffusion method for L. monocytogenes strains were reported. One of the tested microorganisms resulted insensible to all the BEOs; 3 strains showed an inhibition from weak to null and the remaining 4 a variable susceptibility. Among the BEOs tested, one showed a strong activity against four pathogenic strains. Four BEOs revealed weak, moderate or null activity in all the 7 sensitive strains, while for two oils only a weak or no activity was reported. MIC values were 0.625 μL/mL for the most efficient BEO, 2.5 and 5 μL/mL for the other samples that showed moderate inhibition. Experiment results are significantly related to the strains tested (P<0.01), rather than the BEO employed (P>0.01). In conclusion, we can consider BEO as a natural technological hurdle for Listeria monocytogenes in combination with other preservation strategies. Finally, this study underlines the necessity to evaluate the antimicrobial activity of EOs on a significant strains number of the same bacteria.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the antibacterial activity of bergamot essential oils on different Listeria monocytogenes strains

et al. 2016

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“…On other hand, the global increase in the incidence, prevalence, and antimicrobial resistance are being reported in recent years. Multiple drug resistance of bacterial pathogens severely limiting therapeutic option for infections in both animals and humans has increased concerns of food-borne diseases [18,2,19]. The use of antimicrobials as prophylaxis or sub-therapeutic level in food producing animals has been a great concern, because it is believed to be an important factor in the emergence of strains of resistant to certain antimicrobials and markedly increase the human health risk associated with consumption of contaminated meat products [2,20,21].…”

Section: Introductionmentioning

confidence: 99%

Occurrence and antibiotic susceptibility of Listeria monocytogenes along meat production chain in southwest Ethiopia

St¹,

Gume²,

Getaneh³

et al. 2020

Preprint

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Background The widespread habit of consumption of raw meat along with poor hygiene during handling, transportation and processing are the major contributors to the high prevalence of food-borne diseases in Ethiopia. Listeriosis is one of the important food-borne diseases acquired through consumption of meat. The aim of this study was to assess the occurrence and antimicrobial susceptibility of Listeria species in meat collected from abattoir and butcher shops.Methods A cross-sectional study was conducted from January 2018 to February 2019 on isolation, phenotypic characterization, and determination of antibiotic susceptibility profiles of isolates of L. monocytogenes. A total of 100 meat samples were collected from abattoir and butcher shops in Jimma Town. Laboratory based isolation and characterization of the Listeria species was made using conventional culture and biolog. Antimicrobial susceptibility test was conducted using ten different selected antibiotics of current veterinary and human therapy. An observational check list was used to assess meat handling practices of butcher shops and abattoir workers.Results From the total of 100 samples collected from butcher shops and the abattoir, 29 (29%; 95% CI: 21.0-38.5%) were found positive for Listeria species. Out of this, 15 (30%, 95% CI: 34.4–68.6%) samples were from the butcher shops and 14 (28%, 95% CI: 31.4–65.6%) were from the abattoir. L. innocua (48.3%) was the dominant species followed by L. seeligeri (27.6%). L. monocytogenes accounts 7% of the isolates. Antibiotic susceptibility tests revealed that L. monocytogenes isolates were susceptible to gentamicin, vancomycin, Sulfamethoxazole-Sulfamethrimethoprim, chloramphenicol and cloxacillin. In contrary, L. monocytogenes isolates were resistant to cloxacillin, penicillin, nalidixic acid and tetracycline. On the other hand, low level of education, lack of training on meat handling and poor cleanliness of meat contact surfaces were significantly associated with the occurrence of Listeria spp. (P < 0.05).Conclusion The findings highlighted the need for regular training on hygienic meat handling practices for meat handlers in order to minimize the risk of food borne diseases. Furthermore, regulation of the optimal use of antimicrobials in food producing animals is highly recommended so as to reduce antimicrobial resistance.

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“…Many multidrug-resistant L. monocytogenes strains have been isolated from foods, dairy farms and human listeriosis cases in previous studies (Srinivasan et al 2005;Jamali et al 2013;Osman et al 2014;Su et al 2016). The results of this study suggest the presence of a high level of resistance to cephalosporines (CAZ, CEP, FOX, CRO), and aminoglycosides (K, CN).…”

Section: Discussionmentioning

confidence: 53%

“…; Su et al . ). The results of this study suggest the presence of a high level of resistance to cephalosporines (CAZ, CEP, FOX, CRO), and aminoglycosides (K, CN).…”

Section: Discussionmentioning

confidence: 97%

Characterization ofListeria monocytogenesfrom encephalitis cases of small ruminants from different geographical regions, in Greece

Kotzamanidis¹,

Papadopoulos

Vafeas³

et al. 2019

J Appl Microbiol

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Aims The aim of this study was to evaluate the genetic diversity and resistance phenotypes of Listeria monocytogenes strains isolated from clinical encephalitis cases, and compare this population to isolates derived from tank milk of healthy animals. Methods and Results A total of 57 L. monocytogenes strains isolated from ruminant's listeriosis cases (n = 31) and from tank milk of healthy ruminants (n = 26) were characterized by species PCR, molecular serotyping, PCR detection of virulence genes, pulsed‐field gel electrophoresis and antimicrobial susceptibility testing. All strains possessed inlA, inlC, inlJ, plcA, actA, hlyA and iap virulence‐associated genes while serotyping analysis revealed that they were mainly assigned into IVb group. Genotyping revealed 50 pulsotypes among the 57 strains assigned into seven clusters while indistinguishable pulsotypes between clinical and milk strains were not identified. Resistance of L. monocytogenes isolates to 14–16 antimicrobial agents tested was observed and 23 antimicrobial resistance profiles (ARPs) were defined while no apparent predominant ARP type was observed among isolates. Conclusions Small ruminants are exposed to a broad range of antimicrobial‐resistant as well as genetically diverse strains of L. monocytogenes carrying virulence‐associated genes but not all of them associated with the disease. Pulsed‐field gel electrophoresis analysis suggests that pulsotypes associated with encephalitis are found in farms only in association with listeriosis. Significance and Impact of the Study These findings are valuable in understanding the ecology of this important food‐borne pathogen and creating awareness for the emerging antimicrobial resistance.

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Molecular characterization and antimicrobial susceptibility of Listeria monocytogenes isolated from foods and humans

Cited by 34 publications

References 47 publications

Evaluation of the antibacterial activity of bergamot essential oils on different Listeria monocytogenes strains

Evaluation of the antibacterial activity of bergamot essential oils on different Listeria monocytogenes strains

Occurrence and antibiotic susceptibility of Listeria monocytogenes along meat production chain in southwest Ethiopia

Characterization ofListeria monocytogenesfrom encephalitis cases of small ruminants from different geographical regions, in Greece

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Molecular characterization and antimicrobial susceptibility of Listeria monocytogenes isolated from foods and humans

Cited by 34 publications

References 47 publications

Evaluation of the antibacterial activity of bergamot essential oils on different Listeria monocytogenes strains

Evaluation of the antibacterial activity of bergamot essential oils on different Listeria monocytogenes strains

Occurrence and antibiotic susceptibility of Listeria monocytogenes along meat production chain in southwest Ethiopia﻿

Characterization ofListeria monocytogenesfrom encephalitis cases of small ruminants from different geographical regions, in Greece

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Occurrence and antibiotic susceptibility of Listeria monocytogenes along meat production chain in southwest Ethiopia