Characterization of<i>Listeria monocytogenes</i>from encephalitis cases of small ruminants from different geographical regions, in Greece

Kotzamanidis, Christos; Papadopoulos, T.; Vafeas, George; Tsakos, P.; Giantzi, Virginia; Ζdragas, Αntonios

doi:10.1111/jam.14244

Cited by 11 publications

(9 citation statements)

References 38 publications

(91 reference statements)

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“…213 Among the 13 serovars, 1/2a, 1/2b, and 4b are the most commonly identified in human and animal clinical isolates, with a noticeable preponderance of serotype 4b in major listeriosis outbreaks 157,266,278 and ruminant neurolisteriosis cases. 155,164,251,308 All 3 serotypes, apart from being implicated in disease, were additionally isolated from food, food processing and farm environments, and animal feces. 26,89,102,152,211,234,277 More recently, molecular typing methods such as pulsed field gel electrophoresis (PFGE), 36,106,123 multilocus sequence typing (MLST), 114 and whole-genome sequencing (WGS) 161 (reviewed in Datta and Burall 54 and Datta et al 55 ) have been employed to link clinical, food, and environmental isolates in epidemiological investigations during outbreaks (PFGE), to study genetic relatedness in Lm populations (MLST), or both (WGS).…”

Section: Lm Strain Heterogeneitymentioning

confidence: 99%

Listeria monocytogenes at the interface between ruminants and humans: A comparative pathology and pathogenesis review

2021

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The bacterium Listeria monocytogenes ( Lm) is widely distributed in the environment as a saprophyte, but may turn into a lethal intracellular pathogen upon ingestion. Invasive infections occur in numerous species worldwide, but most commonly in humans and farmed ruminants, and manifest as distinct forms. Of those, neuroinfection is remarkably threatening due to its high mortality. Lm is widely studied not only as a pathogen but also as an essential model for intracellular infections and host-pathogen interactions. Many aspects of its ecology and pathogenesis, however, remain unclear and are rarely addressed in its natural hosts. This review highlights the heterogeneity and adaptability of Lm by summarizing its association with the environment, farm animals, and disease. It also provides current knowledge on key features of the pathology and (molecular) pathogenesis of various listeriosis forms in naturally susceptible species with a special focus on ruminants and on the neuroinvasive form of the disease. Moreover, knowledge gaps on pathomechanisms of listerial infections and relevant unexplored topics in Lm pathogenesis research are highlighted.

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Section: Lm Strain Heterogeneitymentioning

confidence: 99%

Listeria monocytogenes at the interface between ruminants and humans: A comparative pathology and pathogenesis review

2021

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“…The interaction of internalin with its receptor, E-cad, has been the focus of several studies [35, 46, 47]. InlA has been used as a virulence marker for detecting L. monocytogenes in food [8, 23, 28, 41, 50].…”

Section: Introductionmentioning

confidence: 99%

Characterization of internalin genes in Listeria monocytogenes from food and humans, and their association with the invasion of Caco-2 cells

Cao

Zhang

et al. 2019

Gut Pathog

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Background Internalins are surface proteins that are utilized by Listeria monocytogenes to facilitate its invasion into human intestinal epithelial cells. The expression of a full-length InlA is one of essential virulence factors for L. monocytogenes to cross the intestinal barrier in order to invade epithelial cells. Results In this study, the gene sequences of inlA in 120 L. monocytogenes isolates from food (n = 107) and humans (n = 13) were analyzed. Premature stop codon (PMSC) mutations in inlA were identified in 51 isolates (50 from food and 1 from human). Six mutation types of PMSCs were identified. Among the 51 isolates with PMSCs in inlA , there were 44 serogroup 1/2c, 3c isolates from food, of which seven belonged to serogroups 1/2a, 3a. A total of 153,382 SNPs in 2247 core genes from 42 genomes were identified and used to construct a phylogenetic tree. Serotype 1/2c isolates with inlA PMSC mutations were grouped together. Cell culture studies on 21 isolates showed that the invasion to Caco-2 cells was significantly reduced among isolates with inlA PMSC mutations compared to those without PMSC mutations (P < 0.01). The PMSC mutations in inlA correlated with the inability of the L. monocytogenes isolates to invade Caco-2 cells (Pearson’s coefficient 0.927, P < 0.01). Conclusion Overall, the study has revealed the reduced ability of L. monocytogenes to invade human intestinal epithelial cells in vitro was linked to the presence of PMSC mutations in inlA . Isolates with PMSC mutations shared the same genomic characteristics indicating the genetic basis on the potential virulence of L. monocytogenes invasion. Electronic supplementary material The online version of this article (10.1186/s13099-019-0307-8) contains supplementary material, which is available to authorized users.

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“…The Listeria spp.-specific marker prs was detected in all L. monocytogenes isolates. In Greece, a limited number of studies have provided characterization data (serotypic and genotypic diversity, carriage of virulence genes, antibiotic resistance or biofilm-forming ability) of L. monocytogenes strains isolated from different sources such as meat, meat products, meat-processing environment and vegetables [42,43], soft whey-cheese and related food-processing surfaces [44], veterinary samples and raw ovine milk [19] or human clinical cases [45]. However, to our knowledge, data on L. monocytogenes isolates from bovine milk in Greece are not available.…”

Section: Pcr-serogrouping Of the L Monocytogenes Isolatesmentioning

confidence: 99%

Prevalence, Serotypes, Antimicrobial Resistance and Biofilm-Forming Ability of Listeria monocytogenes Isolated from Bulk-Tank Bovine Milk in Northern Greece

Angelidis,

Grammenou,

Kotzamanidis

et al. 2023

Pathogens

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The prevalence of Listeria monocytogenes in bovine bulk-tank milk (BTM) in Greece has not been previously investigated. The aim of the study was to estimate the prevalence of L. monocytogenes in bovine BTM in Greece and to characterize the isolates in terms of carriage of genes encoding for pathogenic determinants, assess the isolates’ biofilm-forming ability and determine their susceptibility against 12 antimicrobials. Samples (n = 138) of bovine BTM were obtained from farms located throughout Northern Greece and were analyzed qualitatively and quantitatively for L. monocytogenes. Five samples (3.6%) tested positive for L. monocytogenes. The pathogen’s populations in these positive samples were below 5 CFU/mL. Most isolates belonged to the molecular serogroup “1/2a, 3a”. All isolates carried the virulence genes inlA, inlC, inlJ, iap, plcA and hlyA, but actA was detected in only three isolates. The isolates displayed weak to moderate biofilm-forming ability and distinct antimicrobial resistance profiles. All isolates were characterized as multidrug resistant, with resistance to penicillin and clindamycin being a common feature. Considering that L. monocytogenes constitutes a serious public health threat, the key findings of the study, related to the carriage of virulence genes and multidrug resistance, highlight the importance of continued monitoring of the pathogen in farm animals.

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Characterization ofListeria monocytogenesfrom encephalitis cases of small ruminants from different geographical regions, in Greece

Cited by 11 publications

References 38 publications

Listeria monocytogenes at the interface between ruminants and humans: A comparative pathology and pathogenesis review

Listeria monocytogenes at the interface between ruminants and humans: A comparative pathology and pathogenesis review

Characterization of internalin genes in Listeria monocytogenes from food and humans, and their association with the invasion of Caco-2 cells

Prevalence, Serotypes, Antimicrobial Resistance and Biofilm-Forming Ability of Listeria monocytogenes Isolated from Bulk-Tank Bovine Milk in Northern Greece

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