Molecular Characterization And Antimicrobial Effect Of Some Antibiotics On Aeromonas hydrophila Isolated From Different Sources

Abstract

The global assessment of the spread of treatment resistance among Aeromonas hydrophila identified this bacterium as one of the most opportunistic human diseases. So, the purpose of this study was to evaluate antibiotic resistance of 44 Aeromonas hydrophila. (A. hydrophila) isolates previously isolated from different samples representing African catfish (Clarias gariepinus); cow's milk; beef; ground water and diarrheic human stool. In addition, determination of some antimicrobial resistant genes was carried out. The findings showed that all A. hydrophila were resistant to ampicillin, then to methicillin, oxacillin, and amoxicillin, as well as to cefotaxime, tetracycline, streptomycin, and co-trimethoprim. At the same time, they were highly sensitive to meropenem followed by norfloxacin, ciprofloxacin; gentamicin; florphenicol and doxycycline. Antimicrobial resistant-blaTEM and bla CTX-M genes were determined in seven out of eight studied strains of 516 bp., and 593 bp., respectively. Meanwhile, sul1and aadA1 antimicrobial resistant genes were detected in all eight studied strains giving products of 433 bp. and 484 bp., respectively. Six of A. hydrophila strains revealed tet A(A) resistance gene at 576 bp. Therefore, this study concluded that, consumers may have public health concerns if antibiotic resistant A. hydrophila strains are found in foods derived from animals (meat, milk), African catfish, and groundwater.

Phenotypic Characteristics of Aeromonas hydrophila Isolated from the Wild African Catfish (Clarias gariepinus) in the River Nile, Egypt

“…The present data from the examination of 100 wild C. gariepinus with prevalence of 40% of MAS within this study showed that A. hydrophila was sensitive to gentamicin and ciprofloxacin, while they were highly resistant to Penicillins and Tetracycline. These results nearly coincide with other studies (Nasser et al, 2022;Austin et al, 2012). This study confirmed that A. hydrophila is resistant to β-lactam antimicrobials, and their effectiveness has considerably declined because of the production of βlactamases and other resistant genes.…”