Molecular characteristics of extended-spectrum β-lactamase-producing Escherichia coli in Japan: emergence of CTX-M-15-producing E. coli ST131

Kuroda, Hiroshi; Yano, Hisakazu; Hirakata, Yoichi; Arai, Kohei; Endo, Shiro; Kanamori, Hajime; Yamamoto, Hiromi; Ichimura, Sachiko; Ogawa, Miho; Shimojima, Masayuki; Komatsu, Mayumi; Jonai, Teruko; Itagaki, Sachiko; Nonomiya, Yuriko; Suwabe, Akira; Kaku, Mitsuo

doi:10.1016/j.diagmicrobio.2012.06.011

Cited by 26 publications

(32 citation statements)

References 9 publications

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“…bla CTX-M-15 -carrying plasmids, particularly found in B2-O25b:H4-ST131 E. coli, have been spreading globally. 16,27 The bla CTX-M-15 genes are commonly found in large plasmids that often carry other antimicrobial-resistant genes, including bla TEM-1 , tetA, bla OXA-1 , and aac(6¢)-lb-cr, classifying most of them as members of incompatibility group F. 8 In this study, however, bla CTX-M-15 -harboring E. coli belong to phylogenetic group D and ST38, not B2-O25b:H4-ST131. In France, the bla CTX-M-15 -carrying plasmids from cattle were derived from non-ST131 E. coli isolates that were highly similar to those found in ST131 E. coli isolates in humans.…”

Section: Discussionmentioning

confidence: 97%

“…In France, the bla CTX-M-15 -carrying plasmids from cattle were derived from non-ST131 E. coli isolates that were highly similar to those found in ST131 E. coli isolates in humans. 21 bla CTX-M-15 -carrying plasmids from human clinical isolates derived from ST131 E. coli have recently been reported in Japan, 16,22 although the molecular structure of the plasmids has not been examined. Therefore, …”

Section: Discussionmentioning

confidence: 98%

“…In recent years, the most prevalent type of ESBLs has been CTX-M. 29 In particular, CTX-M-15, a member of the CTX-M-1 group, is the most widely disseminated ESBL globally. 16,29 Since the report of Swann et al 3 , the spreading of antimicrobial-resistant bacteria and genes from food-producing animals to humans has been investigated. Nationwide surveillance studies have indicated that antimicrobial resistances have been harbored in food-producing animals.…”

Section: Introductionmentioning

confidence: 99%

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The Role of Flies in Spreading the Extended-Spectrum β-lactamase Gene from Cattle

Usui

Iwasa

Fukuda

et al. 2013

Microbial Drug Resistance

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The spreading of antimicrobial-resistant bacteria and genes from food-producing animals to humans has been a subject of increasing concern. To clarify the role of flies in spreading the extended-spectrum β-lactamase (ESBL) gene from food-producing animals to humans, we isolated and characterized a third-generation cephalosporin-resistant Escherichia coli strain from flies and cattle feces from a cattle barn. Cephalosporin-resistant strains were isolated from 14.3% (13/91) of houseflies, 10.3% (7/68) of false stable flies, and 7.5% (7/93) of cattle feces. Twenty-seven cephalosporin-resistant strains were tested for the presence of antimicrobial resistance genes. Of the 27 samples, 22 isolates from 11 houseflies, 5 false stable flies, and 6 cattle feces samples harbored the blaCTX-M-15 gene. All blaCTX-M-15-harboring isolates belonged to phylogenetic group D and the ST38 clonal group. Analysis of pulsed-field gel electrophoresis showed that these isolates were divided into two clusters, indicating that flies carried several of the same clones that were detected in cattle feces. All blaCTX-M-15 gene-harboring plasmids were transferable and were members of incompatibility group FIB. These results suggest that transferable plasmids encoding ESBL were prevalent among flies and cattle. As vectors, flies may play an important role in spreading ESBL-producing bacteria from food-producing animals to humans.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Role of Flies in Spreading the Extended-Spectrum β-lactamase Gene from Cattle

Usui

Iwasa

Fukuda

et al. 2013

Microbial Drug Resistance

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“…8 42 In particular, CTX-M-15 belonging to the CTX-M-1 group is widely distributed in most European countries, 8,28 North and South America, 23,45 and some Asian countries, 18 including Japan. 25,33,42 Recently, an E. coli clone O-antigen type 25 (O25):H-antigen type 4 (H4), sequence type 131 (ST131) and the B2 phylogenetic group, producing CTX-M-15 with high virulence potential, has been reported as a major public health problem worldwide. 18,23,25,28,41,45 The detection of plasmid replicon types is an important tool to trace the diffusion of plasmids conferring antimicrobial resistance and to follow the evolution and spread of emerging plasmids.…”

Section: Introductionmentioning

confidence: 99%

“…25,33,42 Recently, an E. coli clone O-antigen type 25 (O25):H-antigen type 4 (H4), sequence type 131 (ST131) and the B2 phylogenetic group, producing CTX-M-15 with high virulence potential, has been reported as a major public health problem worldwide. 18,23,25,28,41,45 The detection of plasmid replicon types is an important tool to trace the diffusion of plasmids conferring antimicrobial resistance and to follow the evolution and spread of emerging plasmids. A formal scheme of plasmid classification is based on the incompatibility (Inc) group.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characteristics of Extended-Spectrum β-Lactamase–ProducingEscherichia coliin a University Teaching Hospital

OsawaKayo

ShigemuraKatsumi

ShimizuRika

et al. 2015

Microbial Drug Resistance

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The prevalence of extended-spectrum β-lactamases (ESBLs) has been increasing worldwide. Recently, a pandemic clone of Escherichia coli O25:H4, sequence type 131 (ST131), producing ESBL-type CTX-M-15 has been reported as a major problem. In this study, we investigated the molecular characteristics of 72 ESBL-producing E. coli isolates. We detected the ESBL blaCTX-M gene and nine virulence factor genes (papC, papEF, fimH, hlyA, iutA, sfa, eaeA, bfpA, and aggR) by PCR and DNA sequencing, plasmid replicon typing, phylogenetic grouping, repetitive-sequence-based PCR (rep-PCR), and multilocus sequence typing. All strains were positive for blaCTX-M. Twenty-two (30.6%) strains in CTX-M-1 group included 9 (12.5%) of CTX-M-15, 3 (4.2%) in CTX-M-2 group, and 47 (65.3%) strains in CTX-M-9 group. The CTX-M-15-producing E. coli O25:H4 ST131 was derived from phylogenetic group B2 and rep-PCR pattern d. The most prevalent virulence factor was fimH (72 strains; 100%) and the most common replicon type was the IncF type (65 strains; 90.3%). The CTX-M-9 group was significantly associated with the presence of papC and papEF [OR (95% CI)=9.22 (1.32-64.7), p=0.025] or hlyA [OR (95% CI)=5.57 (1.17-26.4), p=0.031]. In conclusion, we confirmed that CTX-M-15-producing E. coli O25:H4 ST131 has emerged in Japan and found significant virulence factors with CTX-M-9 group.

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Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in cattle production – a threat around the world

Palmeira

Ferreira

2020

Heliyon

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Food producing animal is a global challenge in terms of antimicrobial resistance spread. Extended-spectrum betalactamase (ESBL)-producing Enterobacteriaceae are relevant opportunistic pathogens that may spread in many ecological niches of the One Health approach as human, animal and environment due to intestinal selection of antimicrobial resistant commensals in food production animals. Cattle production is a relevant ecological niche for selection of commensal bacteria with antimicrobial resistance from microbiota. Enterobacteriaceae show importance in terms of circulation of resistant-bacteria and antimicrobial resistance genes via food chain creating a resistance reservoir, setting up a threat for colonization of humans and consequent health risk. ESBL-producing Enterobacteriaceae are a threat in terms of human health responsible for life threatening outbreaks and silent enteric colonization of community populations namely the elder population. Food associated colonization is a risk difficult to handle and control. In a time of globalization of food trading, population intestinal colonization is a mirror of food production and in that sense this work aims to make a picture of ESBL-producing Enterobacteriaceae in animal production for food over the world in order to make some light in this reality of selection of resistant threats in food producing animal.

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Molecular characteristics of extended-spectrum β-lactamase-producing Escherichia coli in Japan: emergence of CTX-M-15-producing E. coli ST131

Cited by 26 publications

References 9 publications

The Role of Flies in Spreading the Extended-Spectrum β-lactamase Gene from Cattle

The Role of Flies in Spreading the Extended-Spectrum β-lactamase Gene from Cattle

Molecular Characteristics of Extended-Spectrum β-Lactamase–ProducingEscherichia coliin a University Teaching Hospital

Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in cattle production – a threat around the world

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