Molecular characteristics of early-stage female germ cells revealed by RNA sequencing of low-input cells and analysis of genome-wide DNA methylation

Ma, Baoshan; Lee, Tin‐Lap; Hu, Baishi; Li, Jing; Li, Xiaoyong; Zhao, Xiaodong; Hou, Changliang; Zhang, Chen; Huang, Xingxu; Chen, Xuejin; Wu, Ji

doi:10.1093/dnares/dsy042

Cited by 15 publications

(21 citation statements)

References 54 publications

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“…Among these, hif1an , which is related to Notch signaling, could be a potential marker for female germline cells. Our findings indicated that FGSCs belong to ASCs in the high-order organization, and further confirmed the existence of FGSCs, consistent with the previous reports for cellular and molecular characteristic 4–7,44,45 .…”

Section: Discussionsupporting

confidence: 92%

Three-dimensional Genome Structure Reveals Distinct Chromatin Signatures of Mouse Female Germline Stem Cells During Development

Tian

Zhao

Xie

et al. 2019

Preprint

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2 0 3 1 conformation analysis. We also showed that the X chromosome structures were 3 2 similar in spermatogonial stem cells and FGSCs. Using integrative analysis of the 3 3 three-dimensional chromatin structure, we observed that the TADs were attenuated in 3 4 germinal vesicle oocytes and disappeared in metaphase II oocytes during FGSC 3 5 development. Finally, we identified conserved compartments belonging to the 3 6 paternal/maternal genomes during early embryonic development, which were related 3 7to imprinted genes. These results will provide a valuable resource for studying and 3 8 further our understanding of the fundamental characteristics of oogenesis and early 3 9 embryo development. 4 0 4 1

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Section: Discussionsupporting

confidence: 92%

Three-dimensional Genome Structure Reveals Distinct Chromatin Signatures of Mouse Female Germline Stem Cells During Development

Tian

Zhao

Xie

et al. 2019

Preprint

Self Cite

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“…Time course RNA sequencing has been widely used to study cellular differentiation (Ma et al, 2018), tissue development (Cardoso-Moreira et al, 2019), and aging (Baumgart et al, 2016). We provide a new insight on the transcriptome changes in chickens between different development stages of BM and AF.…”

Section: Discussionmentioning

confidence: 99%

“…WGCNA is a powerful tool for identifying genes that are associated with the phenotypes under study ( Langfelder and Horvath, 2008 ). WGCNA can also be used to identify tissue- or stage-specifically expressed gene modules ( Gao et al, 2018 ; Ma et al, 2018 ). To investigate the expressed genes in different developmental stages, we performed the WGCNA for BM and AF tissues as a consensus data set.…”

Section: Discussionmentioning

confidence: 99%

RNA-Seq Analysis Reveals Hub Genes Involved in Chicken Intramuscular Fat and Abdominal Fat Deposition During Development

et al. 2020

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Fat traits are important in the chicken industry where there is a desire for high intramuscular fat (IMF) and low abdominal fat. However, there is limited knowledge on the relationship between the dynamic status of gene expression and the body fat deposition in chicken. Transcriptome data were obtained from breast muscle and abdominal fat of female chickens from nine developmental stages (from embryonic day 12 to hatched day 180). In total, 8,545 genes in breast muscle and 6,824 genes in abdominal fat were identified as developmentally dynamic genes. Weighted correlation network analysis was used to identify gene modules and the hub genes. Twenty-one hub genes were identified, e.g., ENSGALG00000041996, which represents a candidate for high IMF, and CREB3L1, which relates to low abdominal fat weight. The transcript factor L3MBTL1 and the transcript factor cofactors TNIP1, HAT1, and BEND6 related to both high breast muscle IMF and low abdominal fat weight. Our results provide a resource of developmental transcriptome profiles in chicken breast muscle and abdominal fat. The candidate genes can be used in the selection for increased IMF content and/or a decrease in abdominal fat weight which would contribute to the improvement of these traits.

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“…In our experiments, Kunming mice were chosen to construct the POF models in view of their excellent reproductive capacity and adaptability. Previous studies [45,46] have shown that FGSCs not only present in the ovaries of Kunming mice, but also exist in CD-1 and C57 mice. We believe RES is also as effective in other strains.…”

Section: Discussionmentioning

confidence: 99%

Resveratrol Plays a Protective Role against Premature Ovarian Failure and Prompts Female Germline Stem Cell Survival

Jiang

Zhao-yuan

Cha

et al. 2019

IJMS

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This study was designed to investigate the protective effect of resveratrol (RES) on premature ovarian failure (POF) and the proliferation of female germline stem cells (FGSCs) at the tissue and cell levels. POF mice were lavaged with RES, and POF ovaries were co-cultured with RES and/or GANT61 in vitro. FGSCs were pretreated with Busulfan and RES and/or GANT61 and co-cultured with M1 macrophages, which were pretreated with RES. The weights of mice and their ovaries, as well as their follicle number, were measured. Ovarian function, antioxidative stress, inflammation, and FGSCs survival were evaluated. RES significantly increased the weights of POF mice and their ovaries as well as the number of follicles, while it decreased the atresia rate of follicles. Higher levels of Mvh, Oct4, SOD2, GPx, and CAT were detected after treatment with RES in vivo and in vitro. RES treatment resulted in significantly lower TNF-α and IL-6 concentrations and an obviously higher IL-10 concentration in the ovaries. In FGSCs, higher Mvh, Oct4, and SOD2 concentrations and lower TNF-α, IL-6, and MDA concentrations were measured in the RES group. Blockage of the Hh signaling pathway reversed the protective effect of RES on FGSCs. In conclusion, RES effectively improved the ovarian function of the POF model and the productive capacity of FGSCs via relieving oxidative stress and inflammation and a mechanism involving the Hh signaling pathway, suggesting that RES is a potential agent against POF and can aid in the survival of FGSCs.

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Molecular characteristics of early-stage female germ cells revealed by RNA sequencing of low-input cells and analysis of genome-wide DNA methylation

Cited by 15 publications

References 54 publications

Three-dimensional Genome Structure Reveals Distinct Chromatin Signatures of Mouse Female Germline Stem Cells During Development

Three-dimensional Genome Structure Reveals Distinct Chromatin Signatures of Mouse Female Germline Stem Cells During Development

RNA-Seq Analysis Reveals Hub Genes Involved in Chicken Intramuscular Fat and Abdominal Fat Deposition During Development

Resveratrol Plays a Protective Role against Premature Ovarian Failure and Prompts Female Germline Stem Cell Survival

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