Three-dimensional Genome Structure Reveals Distinct Chromatin Signatures of Mouse Female Germline Stem Cells During Development

Abstract

2 0 3 1 conformation analysis. We also showed that the X chromosome structures were 3 2 similar in spermatogonial stem cells and FGSCs. Using integrative analysis of the 3 3 three-dimensional chromatin structure, we observed that the TADs were attenuated in 3 4 germinal vesicle oocytes and disappeared in metaphase II oocytes during FGSC 3 5 development. Finally, we identified conserved compartments belonging to the 3 6 paternal/maternal genomes during early embryonic development, which were related 3 7to imprinted genes. These results will provide a valuable resource for studying and 3 8 further our understanding of the fundamental characteristics of oogenesis and early 3 9 embryo development. 4 0 4 1

Offspring production of haploid spermatid-like cells derived from mouse female germline stem cells with chromatin condensation

“…Germ cells at each stage were sorted by flow cytometry, based on being positive for EGFP or EBFP. Original Hi-C-seq or H3K27me3 and H3K4me3 data for SSCs and FGSCs were obtained from previously published articles [28][29][30].…”