Molecular characterisation of Trypanosoma rangeli strains isolated from Rhodnius ecuadoriensis in Peru, R. colombiensis in Colombia and R. pallescens in Panama, supports a co-evolutionary association between parasites and vectors

Urrea, Daniel Alfonso; Carranza, Julio César; Cuba, César Augusto Cuba; Gurgel-Gonçalves, Rodrigo; Guhl, Felipe; Schofield, Claire; Triana-Chávez, Omar; Vallejo, Gustavo Adolfo

doi:10.1016/j.meegid.2004.07.005

Cited by 50 publications

(37 citation statements)

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“…Subsequent studies clearly showed host-parasite specific associations between T. rangeli-Rhodnius spp. and a hypothesis that these triatomines act as biological filters in the transmission of genetically distinct populations of T. rangeli (11,14,15 (11). Such association agrees with the report of a trypanolytic protein present in R. prolixus hemolymph, which selectively lyses (KP1-) strains isolated from R. pallescens, R. colombiensis and R. ecuadoriensis, but not (KP1+) strains (18).…”

supporting

confidence: 89%

“…Based on the current hypothesis regarding T. rangeli and Rhodnius spp. co-evolution (11,14,15), we propose that infectivity (intestinal, hemolymphatic and glandular) and the transmission of the T. rangeli strain SC-58 (characterized as KP1-) would be greater and more efficient in R. pictipes. Therefore, the goal of the present study was to analyze the susceptibility of four Rhodnius species (R. robustus, R. neglectus, R. nasutus and R. pictipes) to a Brazilian strain of T. rangeli (SC-58/KP1-).…”

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confidence: 99%

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Susceptibilidad de diferentes especies de Rhodnius (Hemiptera, Reduviidae, Triatominae) a una cepa brasileña de Trypanosoma rangeli (SC58/KP1-)

et al. 2014

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Introduction: Specific host-parasite associations have been detected experimentally and suggest that triatomines of the genus Rhodnius act as biological filters in the transmission of Trypanosoma rangeli. Objective: To analyze the susceptibility of four Rhodnius species (Rhodnius robustus, Rhodnius neglectus, Rhodnius nasutus and Rhodnius pictipes) to a Brazilian strain of T. rangeli (SC-58/KP1-). Materials and methods:We selected thirty nymphs of each species, which were fed on blood infected with T. rangeli. Periodically, samples of feces and hemolymph were analyzed. Triatomines with T. rangeli in their hemolymph were fed on mice to check for transmission by bites. Later, the triatomines were dissected to confirm salivary gland infection. Results: Specimens of R. pictipes showed higher rates of intestinal infection compared to the other three species. Epimastigotes and trypomastigotes were detected in hemolymph of four species; however, parasitism was lower in the species of the R. robustus lineage. Rhodnius robustus and R. neglectus specimens did not transmit T. rangeli by bite; after dissection, their glands were not infected. Only one specimen of R. nasutus and two of R. pictipes transmitted the parasite by bite. The rate of salivary gland infection was 16% for R. pictipes and 4% for R. nasutus. Conclusions: Both infectivity (intestinal, hemolymphatic and glandular) and transmission of T. rangeli (SC58/KP1-) were greater and more efficient in R. pictipes. These results reinforce the hypothesis that these triatomines may act as biological filters in the transmission of T. rangeli.

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supporting

confidence: 89%

mentioning

confidence: 99%

Susceptibilidad de diferentes especies de Rhodnius (Hemiptera, Reduviidae, Triatominae) a una cepa brasileña de Trypanosoma rangeli (SC58/KP1-)

et al. 2014

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“…T. rangeli KP1(+) strains have been isolated from domiciliated R. prolixus in Honduras, Colombia and Venezuela, from wild R. neglectus in the Federal District of Brazil (Urrea et al 2005) and wild R. prolixus in Casanare (Colombia) (unpublished data). On the other hand, T. rangeli KP1(-) strains have been isolated from wild R. pallescens from Panama and Colombia, from wild R. colombiensis in the central region of Colombia and from domiciliated R. ecuadoriensis in Peru (Vallejo et al 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Specificity between the vector and the parasite strain which it transmits has been pointed out; each Rhodnius species seems to select the T. rangeli subpopulation to which it is susceptible in nature so that it can then be transmitted to the vertebrate host via insect bite . Nevertheless, a close association has been observed between T. rangeli KP1(+) and vectors from the prolixus group (which includes Rhodnius prolixus and Rhodnius neglectus) and between T. rangeli KP1(-) and vectors from the pallescens group (including Rhodnius pallescens, Rhodnius colombiensis and Rhodnius ecuadoriensis) , Urrea et al 2005. Recent parasite-vector interaction studies have shown a trypanolytic factor in R. prolixus hemolymph impeding the development of T. rangeli KP1(-) strains isolated from R. pallescens, R. colombiensis and R. ecuadoriensis (Sánchez et al 2005).…”

mentioning

confidence: 99%

Preliminary characterization of a Rhodnius prolixus hemolymph trypanolytic protein, this being a determinant of Trypanosoma rangeli KP1(+) and KP1(-) subpopulations' vectorial ability

Pulido

Pérez

Vallejo

2008

Mem. Inst. Oswaldo Cruz

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Rhodnius prolixus is the main Trypanosoma rangeli vector in several Latin-American countries and is susceptible to infection with KP1(+) strains; however, it presents an invasion-resistant response to KP1(-) strains. The present work has identified a trypanolytic protein against T. rangeli KP1(-) in the R. prolixus hemolymph which was fractioned with ammonium sulfate (following dialysis). The results revealed a protein component which did not depend on divalent cations for its biological function whilst keeping its trypanolytic activity at temperatures ranging from -20ºC to 37ºC, at 7.0 to 10.5 pH. The protein was partially purified by gel filtration chromatography and ionic exchange chromatography. The major component presented a molecular weight of around 79 kDa and an isoelectric point between 4.9 and 6.3 and may be directly related to hemolymph trypanolytic activity against T. rangeli KP1(-) populations.Key words: Rhodnius prolixus -Trypanosoma rangeli KP1(+) -Trypanosoma rangeli KP1(-) -trypanolytic factorvectorial ability -insect immunity Trypanosoma rangeli is a hemoflagellate which is apparently apathogenous for humans, presenting pathogenicity for the invertebrate host. T. rangeli is of great interest due to its presence in mixed infection with Trypanosoma cruzi in vertebrates and invertebrate hosts, thereby interfering with a clear diagnosis of Chagas' disease and the epidemiology of these parasites in different Latin-American countries .T. rangeli vectors become infected by ingesting blood from wild or domestic reservoirs in natural conditions. The parasite develops in the digestive tract and then invades the hemolymph where it is recognized by the vector's immune system; however, some strains may escape host defense mechanisms, reaching the salivary glands where they become infective forms or metacyclic trypomastigotes (D'Alessandro-Bacigalupo & Gore-Saravia 1992). Salivary gland invasion is a necessary step for transmission, being mediated by one or several ligandreceptor interactions (Basseri et al. 2002).Previous work has revealed two phylogenetically different groups in T. rangeli called T. rangeli KP1(-) and T. rangeli KP1(+), these being mainly transmitted by the 16 Rhodnius species described so far (Vallejo et al. 2002(Vallejo et al. , 2007. Specificity between the vector and the parasite strain which it transmits has been pointed out; each Rhodnius species seems to select the T. rangeli subpopulation to which it is susceptible in nature so that it can then be transmitted to the vertebrate host via insect bite . Nevertheless, a close association has been observed between T. rangeli KP1(+) and vectors from the prolixus group (which includes Rhodnius prolixus and Rhodnius neglectus) and between T. rangeli KP1(-) and vectors from the pallescens group (including Rhodnius pallescens, Rhodnius colombiensis and Rhodnius ecuadoriensis) , Urrea et al. 2005. Recent parasite-vector interaction studies have shown a trypanolytic factor in R. prolixus hemolymph impeding the development of T. rangeli KP1(...

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“…Colombia is one of the countries in which T. cruzi shares vectors and reservoirs with T. rangeli 7,13,14 . Recently, two important epidemiological groups of T. rangeli have been described: KP1(-) strains, associated with the adaptive line of Rhodnius, represented by R. colombiensis, R. pallescens, and R. ecuadoriensis, and KP1(+) strains, associated with R. prolixus [30][31][32] . These two groups have been defined on the basis of independent mitochondrial (minicircle profile dimorphism obtained by kDNA PCR amplification) and nuclear (mini-exon PCR amplification) markers.…”

Section: Introductionmentioning

confidence: 99%

Detection of Trypanosoma cruzi and Trypanosoma rangeli infection in triatomine vectors by amplification of the histone H2A/SIRE and the sno-RNA-C11 genes

Pavía

Vallejo

Montilla

et al. 2007

Rev. Inst. Med. trop. S. Paulo

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SUMMARYTrypanosoma rangeli is non pathogenic for humans but of important medical and epidemiological interest because it shares vertebrate hosts, insect vectors, reservoirs and geographic areas with T. cruzi, the etiological agent of Chagas disease. Therefore, in this work, we set up two PCR reactions, TcH2AF/R and TrFR2, to distinguish T. cruzi from T. rangeli in mixed infections of vectors based on amplification of the histone H2A/SIRE and the small nucleolar RNA Cl1 genes, respectively. Both PCRs were able to appropriately detect all T. cruzi or T. rangeli experimentally infected-triatomines, as well as the S35/S36 PCR which amplifies the variable region of minicircle kDNA of T. cruzi. In mixed infections, whereas T. cruzi DNA was amplified in 100% of samples with TcH2AF/R and S35/S36 PCRs, T. rangeli was detected in 71% with TrF/R2 and in 6% with S35/S36. In a group of Rhodnius colombiensis collected from Coyaima (Colombia), T. cruzi was identified in 100% with both PCRs and T. rangeli in 14% with TrF/R2 and 10% with S35/S36 PCR. These results show that TcH2AF/R and TrF/R2 PCRs which are capable of recognizing all T. cruzi and T. rangeli strains and lineages could be useful for diagnosis as well as for epidemiological field studies of T. cruzi and T. rangeli vector infections.

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Molecular characterisation of Trypanosoma rangeli strains isolated from Rhodnius ecuadoriensis in Peru, R. colombiensis in Colombia and R. pallescens in Panama, supports a co-evolutionary association between parasites and vectors

Cited by 50 publications

References 24 publications

Susceptibilidad de diferentes especies de Rhodnius (Hemiptera, Reduviidae, Triatominae) a una cepa brasileña de Trypanosoma rangeli (SC58/KP1-)

Susceptibilidad de diferentes especies de Rhodnius (Hemiptera, Reduviidae, Triatominae) a una cepa brasileña de Trypanosoma rangeli (SC58/KP1-)

Preliminary characterization of a Rhodnius prolixus hemolymph trypanolytic protein, this being a determinant of Trypanosoma rangeli KP1(+) and KP1(-) subpopulations' vectorial ability

Detection of Trypanosoma cruzi and Trypanosoma rangeli infection in triatomine vectors by amplification of the histone H2A/SIRE and the sno-RNA-C11 genes

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