Molecular characterisation of the inactive allele of the gene Glu-A1 and the development of a set of AS-PCR markers for HMW glutenins of wheat

Bustos, Alfredo de; Rubio, P.; Jouve, N.

doi:10.1007/s001220051390

Cited by 93 publications

(55 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Hence, the single base alterations and the different insertions/deletions in the B-hordein clones could result in various sizes or structures of prolamin polypeptides. There were numerous nonsynonymous cSNPs among certain B-hordein clones, which can be used to develop allelicspecific markers for selecting the corresponding genes in different hull-less barley accessions, and this method was previously used for c-gliadin (Zhang et al 2003) and for the selection of the HMW-GS genes (De-Bustos et al 2000) in wheat. Since the LMW-GSs make a noticeable contribution to the bread-making quality of wheat, the high homology of the B-hordeins of hull-less barley with the LMW-GSs of T. aestivum may suggest similar breadmaking influences for these B-hordeins.…”

Section: Discussionmentioning

confidence: 99%

Structural and expressional analysis of the B-hordein genes in Tibetan hull-less barley

Han

Deng

et al. 2009

Genetica

View full text Add to dashboard Cite

The B-hordein gene family was analyzed from two Tibetan hull-less barley cultivars Z09 and Z26 (Hordeum vulgare subsp. vulgare). Fourteen B-hordein genes, designated BZ09-2 to BZ09-5 (from Z09) and BZ26-1 to BZ26-10 (from Z26), were sequenced. Seven of them, similar to a previously reported BZ09-1 from Z09, were predicted to encode putative active proteins each with a signal peptide, a repetitive domain, and a C-terminal region; seven of them were predicted to be pseudogenes. The B-hordein gene family was analyzed using all known representatives of B-hordein sequences and two most similar LMW-GSs of Triticum aestivum. Alignment of these seven putative proteins with known B-hordeins and two most similar LMW-GSs of T. aestivum revealed that they shared a common motif. A large variation was observed between numbers of repeat units of predicted B-hordeins of Z26 and Z09. Phylogenetic analysis revealed that all BZ26 clones were clustered in a subgroup, and BZ09-1 formed another subgroup by itself in the putative eight active genes. In addition, six 5 0 -upstream regulatory sequences of the B-hordein genes were isolated from the two accessions by a single oligonucleotide nested PCR, and several different mutations were identified in the cis-acting element GLM and two distinctive sequences (Z09P-2 and Z26P-3). Phylogenetic analysis of 5 0 -upstream regulatory regions of the B-hordein genes showed that members from the same accession were clustered together except for two distinct members. Quantitative real time PCR analysis indicated distinct expression levels of B-hordein genes in four developing stages of developing grains in two accessions. These findings suggest B-hordein genes have intrinsic differences between accessions, and this knowledge will be useful for incorporating the B-hordeins protein in barley breeding programs.

show abstract

Section: Discussionmentioning

confidence: 99%

Structural and expressional analysis of the B-hordein genes in Tibetan hull-less barley

Han

Deng

et al. 2009

Genetica

View full text Add to dashboard Cite

show abstract

“…So the differences in BH1 to BH4 DNA sequences involving insertions and deletions could result in polypeptides with different size or structure in prolamins family, and of these differences, there were numerous nonsynonymous cSNPs, which represented over 50% of the total cSNPs in BH1 to BH4. These differences may be used in developing allelic gene-specific marker for selecting the corresponding genes in hull-less barley, and this molecular marker technology has been successfully used in selection for γ-gliadin [28] and HMW gene [29] in wheat.…”

Section: Discussionmentioning

confidence: 99%

Cloning and Characterization of Four B-hordein Genes from Tibetan Hull-less Barley (Hordeum vulgare subsp. vulgare)

Han

Qian

Pan

et al. 2006

Acta Genetica Sinica

View full text Add to dashboard Cite

“…Biotypes lacking different HMW glutenin subunits were identified in the variety Nap Hal from Nepal. Authors hypothesized that deletions of individual subunits would reduce bread making quality of mutants, which was later confirmed (Bustos et al 2000;Lawrence et al 1988;Rogers et al 2001). Two major genetic mechanisms may lead to inactivation of gluten protein genes.…”

Section: Introductionmentioning

confidence: 89%

Development and characteristics of ω-gliadin-free wheat genotypes

Waga

Skoczowski

2013

Euphytica

View full text Add to dashboard Cite

Omega gliadin proteins are one of the most allergenic components of wheat gluten. Proteins of the x-5 subgroup are recognized as main allergens causing wheat dependent exercise induced anaphylaxis-the most dangerous, life-threatening IgE mediated food allergy. A set of wheat genotypes lacking all x-gliadins has been developed by cumulating inactive gene variants in three gliadin coding loci (Gli A1, Gli B1 and Gli D1), using traditional plant breeding methods. Endosperm proteins of x-gliadin-free genotypes were compared to a control genotype containing all x-gliadins by A-PAGE, SDS-PAGE and RP-HPLC. A considerable decrease (about 30 %) of gliadin immunoreactivity as a consequence of xgliadin elimination was demonstrated by ELISA, using sera of ten patients allergic to gluten. Preliminary evaluation of the technological properties of the xgliadin-free genotype by the SDS sedimentation test suggests that elimination of all x-gliadins may also significantly improve wheat bread making quality.

show abstract

Molecular characterisation of the inactive allele of the gene Glu-A1 and the development of a set of AS-PCR markers for HMW glutenins of wheat

Cited by 93 publications

References 23 publications

Structural and expressional analysis of the B-hordein genes in Tibetan hull-less barley

Structural and expressional analysis of the B-hordein genes in Tibetan hull-less barley

Cloning and Characterization of Four B-hordein Genes from Tibetan Hull-less Barley (Hordeum vulgare subsp. vulgare)

Development and characteristics of ω-gliadin-free wheat genotypes

Contact Info

Product

Resources

About