The Tibetan hulless barley (Hordeum vulgare L. var. nudum), also called "Qingke" in Chinese and "Ne" in Tibetan, is the staple food for Tibetans and an important livestock feed in the Tibetan Plateau. The diploid nature and adaptation to diverse environments of the highland give it unique resources for genetic research and crop improvement. Here we produced a 3.89-Gb draft assembly of Tibetan hulless barley with 36,151 predicted protein-coding genes. Comparative analyses revealed the divergence times and synteny between barley and other representative Poaceae genomes. The expansion of the gene family related to stress responses was found in Tibetan hulless barley. Resequencing of 10 barley accessions uncovered high levels of genetic variation in Tibetan wild barley and genetic divergence between Tibetan and non-Tibetan barley genomes. Selective sweep analyses demonstrate adaptive correlations of genes under selection with extensive environmental variables. Our results not only construct a genomic framework for crop improvement but also provide evolutionary insights of highland adaptation of Tibetan hulless barley.Tibetan hulless barley | Triticeae evolution | genetic diversity | adaptation | selective sweep

We introduce a method to estimate the Hi mass within the optical radius of disk galaxies from integrated HI spectra, with an uncertainty of 0.09 dex. We use these estimates to study how inner Hi fuels star formation in late-type disk galaxies. We find that star formation rate (SFR) at a given stellar mass (M * ) is well correlated with the inner Hi surface density (Σ HI,in ) and inner Hi mass-to-stellar mass ratio. For the massive (M * > 10 10 M ) disk galaxies, higher SFR at a given stellar mass is also related with higher efficiency of converting inner Hi to molecular gas, but no such correlation is found for the total Hi mass. The highest Σ HI,in and the fastest depletion of the total neutral gas within the optical disks are found in the most compact and star-forming disk galaxies at a given stellar mass. These results highlight the important role of inner Hi as an intermediate step of fueling star formation in disk galaxies.

This study aimed to discover the novel noninvasive biomarkers for the diagnosis of pulmonary tuberculosis (TB). We applied iTRAQ 2D LC-MS/MS technique to investigate protein profiles in patients with pulmonary TB and other lung diseases. A total of 34 differentially expressed proteins (24 upregulated proteins and ten downregulated proteins) were identified in the serum of pulmonary TB patients. Significant differences in protein S100-A9 (S100A9), extracellular superoxide dismutase [Cu-Zn] (SOD3), and matrix metalloproteinase 9 (MMP9) were found between pulmonary TB and other lung diseases by ELISA. Correlations analysis revealed that the serum concentration of MMP9 in the pulmonary TB was in moderate correlation with SOD3 (r = 0.581) and S100A9 (r = 0.471), while SOD3 was in weak correlation with S100A9 (r = 0.287). The combination of serum S100A9, SOD3, and MMP9 levels could achieve 92.5% sensitivity and 95% specificity to discriminate between pulmonary TB and healthy controls, 90% sensitivity and 87.5% specificity to discriminate between pulmonary TB and pneumonia, and 85% sensitivity and 92.5% specificity to discriminate between pulmonary TB and lung cancer, respectively. The results showed that S100A9, SOD3, and MMP9 may be potential diagnostic biomarkers for pulmonary TB, and provided experimental basis for the diagnosis of pulmonary TB.

BackgroundAegilops variabilis No.1 is highly resistant to cereal cyst nematode (CCN). However, a lack of genomic information has restricted studies on CCN resistance genes in Ae. variabilis and has limited genetic applications in wheat breeding.ResultsUsing RNA-Seq technology, we generated a root transcriptome at a sequencing depth of 4.69 gigabases of Ae. variabilis No. 1 from a pooled RNA sample. The sample contained equal amounts of RNA extracted from CCN-infected and untreated control plants at three time-points. Using the Trinity method, nearly 52,081,238 high-quality trimmed reads were assembled into a non-redundant set of 118,064 unigenes with an average length of 500 bp and an N50 of 599 bp. The total assembly was 59.09 Mb of unique transcriptome sequences with average read-depth coverage of 33.25×. In BLAST searches of our database against public databases, 66.46% (78,467) of the unigenes were annotated with gene descriptions, conserved protein domains, or gene ontology terms. Functional categorization further revealed 7,408 individual unigenes and three pathways related to plant stress resistance.ConclusionsWe conducted high-resolution transcriptome profiling related to root development and the response to CCN infection in Ae. variabilis No.1. This research facilitates further studies on gene discovery and on the molecular mechanisms related to CCN resistance.

We present a detection of 89 candidates of ultra-diffuse galaxies (UDGs) in a 4.9 degree 2 field centered on the Hickson Compact Group 95 (HCG 95) using deep g-and r-band images taken with the Chinese Near Object Survey Telescope. This field contains one rich galaxy cluster (Abell 2588 at z=0.199) and two poor clusters (Pegasus I at z=0.013 and Pegasus II at z=0.040). The 89 candidates are likely associated with the two poor clusters, giving about 50 − 60 true UDGs with a half-light radius r e > 1.5 kpc and a central surface brightness µ(g, 0) > 24.0 mag arcsec −2 . Deep z ′ -band images are available for 84 of the 89 galaxies from the Dark Energy Camera Legacy Survey (DECaLS), confirming that these galaxies have an extremely low central surface brightness. Moreover, our UDG candidates are spread over a wide range in g − r color, and ∼26% are as blue as normal star-forming galaxies, which is suggestive of young UDGs that are still in formation. Interestingly, we find that one UDG linked with HCG 95 is a gas-rich galaxy with H I mass 1.1×10 9 M ⊙ detected by the Very Large Array, and has a stellar mass of M ⋆ ∼ 1.8 × 10 8 M ⊙ . This indicates that UDGs at least partially overlap with the population of nearly dark galaxies found in deep H I surveys. Our results show that the high abundance of blue UDGs in the HCG 95 field is favored by the environment of poor galaxy clusters residing in H I-rich large-scale structures.

In this Letter, we investigate how galaxy mass assembly mode depends on stellar mass M * , using a large sample of ∼10, 000 low redshift galaxies. Our galaxy sample is selected to have SDSS R 90 > 5 ′′ .0, which allows the measures of both the integrated and the central NUV−r color indices. We find that: in the M * − ( NUV−r) green valley, the M * < 10 10 M ⊙ galaxies mostly have positive or flat color gradients, while most of the M * > 10 10.5 M ⊙ galaxies have negative color gradients. When their central D n 4000 index values exceed 1.6, the M * < 10 10.0 M ⊙ galaxies have moved to the UV red sequence, whereas a large fraction of the M * > 10 10.5 M ⊙ galaxies still lie on the UV blue cloud or the green valley region. We conclude that the main galaxy assembly mode is transiting from "the outside-in" mode to "the inside-out" mode at M * < 10 10 M ⊙ and at M * > 10 10.5 M ⊙ . We argue that the physical origin of this is the compromise between the internal and the external process that driving the star formation quenching in galaxies. These results can be checked with the upcoming large data produced by the on-going IFS survey projects, such as CALIFA, MaNGA and SAMI in the near future.

The epidemic of pulmonary tuberculosis (TB), especially multidrug-resistance tuberculosis (MDR-TB) presented a major challenge for TB treatment today. We performed iTRAQ labeling coupled with two-dimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS) and Solexa sequencing among MDR-TB patients, drug-sensitive tuberculosis (DS-TB) patients, and healthy controls. A total of 50 differentially expressed proteins and 43 differentially expressed miRNAs (fold change >1.50 or <0.60, P<0.05) were identified in the MDR-TB patients compared to both DS-TB patients and healthy controls. We found that 22.00% of differentially expressed proteins and 32.56% of differentially expressed miRNAs were related, and could construct a network mainly in complement and coagulation cascades. Significant differences in CD44 antigen (CD44), coagulation factor XI (F11), kininogen-1 (KNG1), miR-4433b-5p, miR-424-5p, and miR-199b-5p were found among MDR-TB patients, DS-TB patients and healthy controls (P<0.05) by enzyme-linked immunosorbent assay (ELISA) and SYBR green qRT-PCR validation. A strong negative correlation, consistent with the target gene prediction, was found between miR-199b-5p and KNG1 (r=-0.232, P=0.017). Moreover, we established the MDR-TB diagnostic model based on five biomarkers (CD44, KNG1, miR-4433b-5p, miR-424-5p, and miR-199b-5p). Our study proposes potential biomarkers for MDR-TB diagnosis, and also provides a new experimental basis to understand the pathogenesis of MDR-TB.

Previous findings show that the existence of dense cores or bulges is the prerequisite for quenching a galaxy, leading to a proposed two-step quenching scenario: compaction and quenching. In this scenario, galaxies first grow their cores to a stellar mass surface density threshold and subsequently quenching occurs, suggesting that galaxies evolve from extended star-forming galaxies (eS-FGs), through compact star-forming galaxies (cSFGs), to quenched population. In this work, we aim at examining the possible evolutionary link between eSFGs and cSFGs by identifying the trends in star formation rate (SFR), gas-phase metallicity and HI content, since one would naturally expect that galaxies evolve along the track of cold gas consumption and metal enhancement. We select a volumelimited sample of 15,933 galaxies with stellar mass above 10 9.5 M ⊙ and redshift of 0.02 < z < 0.05 from the NASA-Sloan-Atlas catalog within the ALFALFA footprint. The cSFGs on average exhibit similar or slightly higher SFRs of ∼0.06 dex and significantly higher gas-phase metallicity (up to 0.2 dex at low mass) with respect to the eSFGs, while the cSFGs dominate the galaxy population of the most intense star formation activities. More importantly, overall the median HI content and gas depletion time of cSFGs are about half of eSFGs. Our result supports the compaction and quenching scenario that galaxies evolve and grow their cores along the track of cold gas consumption and metal enhancement. The environments of eSFGs and cSFGs are indistinguishable, suggesting that the compaction process is independent of any environmental effects at least for low-redshift universe.5 This definition of compact SF galaxies differs from previous works, where compact is an absolute term to identify the smallest galaxies (e.g. van Dokkum et al. 2015). 6 http://www.nsatlas.org/ 7