Molecular characterisation of Musa L. cultivars cultivated in Malawi using microsatellite markers

Changadeya, Wisdom

doi:10.5897/ajb10.1738

Cited by 8 publications

(18 citation statements)

References 39 publications

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“…The major allele frequency of 0.220 (0.100-0.450) generated from SSR markers (Nyine et al 2017) was found similar to the ones (0.171; 0.046-0.454) obtained in this study, and this shows the effectiveness of CDDP markers in exploring the allelic richness of this vital crop. The identified gene diversity of 0.924 (0.782-0.976) was higher than the previously reported ones obtained with SSR markers (Poerba and Ahmad 2010;Changadeya et al 2012;Nyine et al 2017). The identified PIC was high enough and contributed to the resolution of even the closest accessions and genomic groups.…”

Section: Discussionmentioning

confidence: 51%

“…The primers of the CDDP markers demonstrated high level of PIC (0.918) ranging from 0.768 to 0.975, whereas 0.870 with a range of 0.530 to 0.950, were obtained as PIC and mean respectively, from SSR markers (Nyine et al 2017). Also, in a study of 38 triploid accessions analyzed with 19 microsatellite markers, PIC of 0.850 (0.760-0.942) was obtained (Christelova et al 2011;Changadeya et al 2012). In comparison with our findings, lower value of PIC of 0.827 (0.625-0.936) was generated from the analysis of 70 diploid accessions with 19 microsatellite loci (Christelova et al 2011).…”

Section: Discussionmentioning

confidence: 92%

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Genetic Diversity and Population Assessment of Musa L. (Musaceae) Employing CDDP Markers

et al. 2021

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Sixty-six accessions of Musa genus with different genomic groups that consisted of wild relatives and cultivated lines were obtained from the International Transit Center, Belgium, for DNA extraction using Cetyl trimethylammonium bromide method, followed by amplification with Conserved DNA-derived Polymorphism (CDDP) markers for genetic diversity and population assessment. A total of 421 alleles with major allele frequency of 2.051 were detected from the reproducible markers. High genetic diversity (GD, 11.093) and polymorphic information content (0.918) were revealed. The number of polymorphic loci and percentage of polymorphic loci ranged from 59 to 66 and 89.34 to 100, respectively. Using the potential genetic indicators including effective number of alleles, Nei’s genetic diversity, and Shannon’s information index, the AS genomic group was identified to have the highest GD, while the AAA accessions had the lowest GD indices. The GD parameters identified in the accessions were ranked as AS > AAB > AAAA > AA > ABB > wild diploidy > BB > AB > AAA from high to low based on polymorphic loci of the markers. Total intraspecific GD, interspecific GD, and estimate gene flow identified were 0.433, 0.404, and 7.113, respectively. The coefficient of gene differentiation of 0.066 was obtained, indicating 6.57% among the population and 93.43% within the population. Dendrogram analysis produced nine major groups with subgroups at similarity index of 0.814. These CDDP functional gene-based markers were informative and very efficient in resolving GD, and population indices among the banana and plantain accessions of different genomes. The identified CDDP markers might serve as potential tools for selecting suitable training populations for breeding and conservation of Musa species.

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Section: Discussionmentioning

confidence: 51%

Section: Discussionmentioning

confidence: 92%

Genetic Diversity and Population Assessment of Musa L. (Musaceae) Employing CDDP Markers

et al. 2021

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“…The difference in genetic diversity could be attributed to differences in mutation rates within populations occurring in varying geographical areas (Changadeya et al 2012). In addition, different anthropogenic factors and natural phenomenon such as death due to drought, fungal diseases and destruction by elephants have shaped genetic diversity within the populations (Guy (1971;Wilson 1988;Edkins et al 2007).…”

Section: Allelic Diversitymentioning

confidence: 99%

Genetic differentiation and diversity of Adansonia digitata L (baobab) in Malawi using microsatellite markers

et al. 2012

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Baobab (Adansonia digitata L) belonging to Bombacaceae family, is one of the most widely used indigenous priority tree species in sub-Saharan Africa, valued in the cosmetic industry for its seed oil, and powdery fruit pulp for juice making. Baobab has high potential for domestication in southern Africa, therefore understanding its genetic diversity and population structuring is warranted. The study investigated the level of genetic diversity and Mantel's test showed a weak positive insignificant correlation (Z=0.12; P=0.64) between genetic distance among populations and actual distance on the ground implying that geneflow was not directly influenced by isolation by distance. The results suggest that seed distribution and tree improvement should recognise the presence of ecotypes and conservation measures should protect all the populations due to existence of private alleles which are of adaptive importance.

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“…In this sense, different works have been carried out for characterization of SSR markers from M. balbisiana (Buhariwalla et al, 2005;Ravishankar et al, 2013;Wang et al, 2011) and M. acuminata (Amorim et al, 2012;Creste et al, 2006;Crouch et al, 1997Crouch et al, , 1998Miller et al, 2010;Wang et al, 2010), whereas other studies have been focused on the development of SSR markers suitable for analysis of both species and/or hybrid cultivars (Brisibe & Ubi, 2020;Kaemmer et al, 1997;Lagoda et al, 1998;Ravishankar et al, 2012;Silva et al, 2015). In other publications, sets of primers previously developed have been used for molecular characterization of Musa varieties (Bawin et al, 2019;Durai et al, 2018;Grapin et al, 1998), including cultivars from certain regions as Brazil (Creste et al, 2003(Creste et al, , 2004, Malawi (Changadeya et al, 2012), East Africa (Nzawele et al, 2018;Onyango et al, 2008;Perrier et al, 2019), Indonesia (Retnoningsih et al, 2009), the Philippines (dela Cruz et al, 2020) or Tanzania (Mgonja et al, 2020). Moreover, SSRs have been used to obtain a saturated genome map of M. acuminata (Hippolyte et al, 2010), to investigate genetic variability between M. acuminata mutants resistant to salinity (Miri et al, 2014) or to assess the identification of Musa accessions in reference DNA collections (Christelová et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Comparative assessment of microsatellite and retrotransposon‐based markers for genetic characterization of commercial banana cultivars (Musa spp.)

et al. 2021

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Banana cultivars of agronomic interest have been genetically characterized using two different molecular markers. On the one hand, a panel of 14 trinucleotide single sequence repeats (SSRs or microsatellites) was optimized for homogeneous PCR conditions. It was tested with 50 individuals from seven cultivars, yielding 76 alleles and 5.4 ± 1.8 alleles per locus, while the presence of cultivar-exclusive alleles allowed the discrimination of all cultivars. On the other hand, a retrotransposon-based marker system named inter-primer binding site (iPBS) was implemented for the first time in the Musa genus. A total of 120 bands were detected in eight different Musa cultivars, from which 65.8% were polymorphic and 23.3% were cultivar exclusive. Both techniques allowed a cut-off identification of all cultivars studied, but overall, iPBS analysis was a more straightforward and economical choice. Despite the fact that we were unable to distinguish local banana varieties belonging to the same cultivar, new cultivar-specific molecular markers have been developed for the Musa genus, which could be used to guide new breeding programmes and maintain high quality of Plátano de Canarias.

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Molecular characterisation of Musa L. cultivars cultivated in Malawi using microsatellite markers

Cited by 8 publications

References 39 publications

Genetic Diversity and Population Assessment of Musa L. (Musaceae) Employing CDDP Markers

Genetic Diversity and Population Assessment of Musa L. (Musaceae) Employing CDDP Markers

Genetic differentiation and diversity of Adansonia digitata L (baobab) in Malawi using microsatellite markers

Comparative assessment of microsatellite and retrotransposon‐based markers for genetic characterization of commercial banana cultivars (Musa spp.)

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