Molecular characterisation of bla OXA-48 carbapenemase-, extended-spectrum β-lactamase- and Shiga toxin-producing Escherichia coli isolated from farm piglets in India

Nirupama, K. R.; Kumar, O.R. Vinodh; Pruthvishree, B.S.; Sinha, Dharmendra Kumar; Murugan, M.; Krishnaswamy, Narayanan; Singh, Bhoj Raj

doi:10.1016/j.jgar.2018.01.007

Cited by 27 publications

(18 citation statements)

References 19 publications

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“…In addition, 33.3-100% In the present study, large proportions (53.1%) of the isolates possessed ESBL genes and the blaCTX-M, blaSHV and blaTEM genes most frequently detected in E. coli than in K. pneumoniae isolates. ese results are in accordance with the ndings of a previous study conducted in the Eastern Cape Province, South Africa as well as in India [14,[41][42][43][44]. Of great concern ESBL gene determinants detected in the present study was higher than those of a similar study previously conducted in Nigeria [38].…”

Section: Genotypic Typing Of Esbl Producing E Coli and Klebsiella Pnsupporting

confidence: 92%

“…A further objective of the study was to screen E. coli and K. pneumoniae isolates for ESBL antibiotic resistance gene determinants. Despite the fact that ESBL resistance genes have most frequently been detected among E. coli and K. pneumoniae isolated from humans, clinical care and hospital facilities, a number of studies have also reported that ESBL resistance genes are harboured by E. coli and K. pneumoniae strains from food-producing animals, meat products as well as vegetables [9,11,14,38,[40][41][42][43][44]. In most studies, ESBL genes (blaCXT-M, blaTEM, blaSHV, blaOXA and blaCMY) were the most frequently detected resistant determinants in E. coli, K. pneumoniae and Salmonella species [6,14,37].…”

Section: Genotypic Typing Of Esbl Producing E Coli and Klebsiella Pnmentioning

confidence: 99%

“…In most studies, ESBL genes (blaCXT-M, blaTEM, blaSHV, blaOXA and blaCMY) were the most frequently detected resistant determinants in E. coli, K. pneumoniae and Salmonella species [6,14,37]. ESBL isolates that possess these resistant determinants particularly blaCXT-M may pose severe public health complications to humans if consumption in contaminated food products [11,14,[40][41][42][43][44]. pneumoniae isolates from beef samples were very similar despite the differences in their sampling stations and this was in accordance with a previous report [45].…”

Section: Genotypic Typing Of Esbl Producing E Coli and Klebsiella Pnmentioning

confidence: 99%

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Antimicrobial Resistance Factors of Extended-Spectrum Beta-Lactamases Producing Escherichia coli and Klebsiella pneumoniae Isolated from Cattle Farms and Raw Beef in North-West Province, South Africa

Montso

Dlamini

Kumar

et al. 2019

BioMed Research International

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Background. Extended spectrum beta-lactamases (ESBLs) producing Enterobacteriaceae cause severe infections in humans which leads to complicated diseases. There is increasing evidence that cattle contribute to the development and spread of multidrug resistant pathogens and this raises public health concern. Despite this, data on the concurrence of ESBL producing pathogens in cattle, especially in the North-West province are rare. Therefore, the aim of the present study was to isolate, identify and characterise ESBL producing E. coli and K. pneumoniae species from cattle faeces and raw beef samples. Results. A total of 151 samples comprising 55 faeces samples and 96 raw beef samples were collected and 259 nonreplicative potential isolates of Enterobacteriaceae were obtained. One hundred and ninety-six isolates were confirmed as E. coli (114; 44%) and K. pneumoniae (82; 32%) species through amplification of uspA and uidA and ntrA gene fragments, respectively. Antimicrobial susceptibility test revealed that large proportions (66.7–100%) of the isolates were resistant to Amoxicillin, Aztreonam, Ceftazidime, Cefotaxime, and Piperacillin and were multidrug resistant isolates. Cluster analysis of antibiotic inhibition zone diameter data revealed close similarities between isolates from different sources or species thus suggested a link in antibiotic exposures. The isolates showing phenotypic resistance against ESBL antimicrobial susceptibility tests were screened for the presence of ESBL gene determinants. It was observed that 53.1% of the isolates harboured ESBL gene determinants. The blaTEM, blaSHV and blaCTX-M genes were detected in E. coli isolates (85.5%, 69.6%, and 58%, respectively) while blaCTX-M and blaOXA were detected in K. pneumoniae (40% and 42.9%, respectively). All the genetically confirmed ESBL producing E. coli and K. pneumoniae isolates were subjected to Enterobacterial Repetitive Intergenic Consensus (ERIC) PCR analysis. Fingerprinting data revealed great similarities between isolates from different areas and sources which indicates cross-contamination between cattle and beef. Conclusion. This study revealed that cattle and its associated food products, beef in particular, harbour ESBL producing pathogens. And this warrants a need to enforce hygiene measures and to develop other mitigation strategies to minimise the spread of antibiotic resistant pathogens from animals to human.

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Section: Genotypic Typing Of Esbl Producing E Coli and Klebsiella Pnsupporting

confidence: 92%

Section: Genotypic Typing Of Esbl Producing E Coli and Klebsiella Pnmentioning

confidence: 99%

Section: Genotypic Typing Of Esbl Producing E Coli and Klebsiella Pnmentioning

confidence: 99%

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Antimicrobial Resistance Factors of Extended-Spectrum Beta-Lactamases Producing Escherichia coli and Klebsiella pneumoniae Isolated from Cattle Farms and Raw Beef in North-West Province, South Africa

Montso

Dlamini

Kumar

et al. 2019

BioMed Research International

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“…Such strains have been reported previously from a pig farm in Germany [17]. Recently in India, E. coli positive for bla NDM-1 and OXA-48 enzymes have been recovered from piglets [9, 18], as well as a mastitis milk sample [19]. More widely, β -lactamase-producing E. coli strains in pigs have been attributed to environmental contamination [20, 21], thus constituting a potential public health threat.…”

Section: Discussionmentioning

confidence: 99%

Epidemiology of carbapenem-resistant Escherichia coli and first report of blaVIM carbapenemases gene in calves from India

et al. 2019

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A cross-sectional study on six dairy farms was conducted to ascertain the occurrence of carbapenem-resistant Escherichia coli in calves. Two-hundred and seventy-nine isolates of E. coli were recovered from 90 faecal samples from apparently healthy (45) and diarrhoeal (45) calves. The isolates were screened for phenotypic susceptibility to carbapenems and production of metallo β-lactamase, as well as five carbapenemase resistance genes by PCR, and overexpression of efflux pumps. Eighty-one isolates (29.03%) were resistant to at least one of three carbapenem antibiotics [meropenem (23.30%), imipenem (2.15%) and ertapenem (1.43%)], and one isolate was positive for the blaVIM gene which was located on an Incl1 plasmid of a novel sequence type (ST 297) by multilocus sequence typing. The majority (83.95%) of isolates had an active efflux pump. Calves housed on concrete floors were approximately seven times more likely to acquire meropenem-resistant isolates than those housed on earthen floors (95% CI 1.27–41.54). In India, carbapenem drugs are not used in food animal treatment, hence carbapenem-resistant strains in calves possibly originate from the natural environment or human contact and is of public health importance. To our knowledge, this is the first report of blaVIM carbapenemases gene in calves from India.

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“…Is important to note that carbapenem-resistant and extendedspectrum β-lactamase (ESBL)-producing E. coli has become widespread in different reservoirs and represent an emerging public health threat (Nirupamaa et al, 2018;Pulss, Semmler, Prenger-Berninghoff, Bauerfeind, & Ewers, 2017;Randall et al, 2017). Some studies have also indicated that the transfer of ESBL-producing bacteria and/or ESBL-encoding genes to humans could happen via the food chain (Ewers, Bethe, Semmler, Guenther, & Wieler, 2012).…”

Section: Discussionmentioning

confidence: 99%

Genotypic and phenotypic characterization of Escherichia coli isolated from mollusks in Brazil and the United States

et al. 2018

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The aim of this study was to determine the serogroups, antimicrobial resistance and genetic diversity of Escherichia coli isolates from samples of bivalve mollusks collected along Santa Catarina coast, Brazil, and from the Chesapeake Bay, Maryland, USA. One hundred forty‐one E. coli isolates were characterized for serogroups with 181 specific O antisera and antimicrobial susceptibility using the disk diffusion method. The genetic diversity was assessed using pulsed‐field gel electrophoresis (PFGE). The results showed that among the isolates, 19.9% were classified as multi‐drug resistant (MDR) and resistance was most frequently observed to cephalothin, nitrofurantoin, and ampicillin. The predominant serogroups were O6, O8, and O38. Some serogroups were recognized as pathogenic E. coli . PFGE dendrograms indicated extensive genetic diversity among the isolates. Although characteristics of the E. coli isolates were highly variable, it is important to note that E. coli belonging to pathogenic serogroups and MDR isolates are present in mollusks of both study areas. This is the first report on the phenotypic and genotypic characterization of E. coli from mollusks from Santa Catarina and the Chesapeake Bay that should encourage studies focusing on comparison of isolates across countries.

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Molecular characterisation of bla OXA-48 carbapenemase-, extended-spectrum β-lactamase- and Shiga toxin-producing Escherichia coli isolated from farm piglets in India

Cited by 27 publications

References 19 publications

Antimicrobial Resistance Factors of Extended-Spectrum Beta-Lactamases Producing Escherichia coli and Klebsiella pneumoniae Isolated from Cattle Farms and Raw Beef in North-West Province, South Africa

Antimicrobial Resistance Factors of Extended-Spectrum Beta-Lactamases Producing Escherichia coli and Klebsiella pneumoniae Isolated from Cattle Farms and Raw Beef in North-West Province, South Africa

Epidemiology of carbapenem-resistant Escherichia coli and first report of blaVIM carbapenemases gene in calves from India

Genotypic and phenotypic characterization of Escherichia coli isolated from mollusks in Brazil and the United States

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