Molecular characterisation and expression of Interleukin-6 and Interleukin-6Δ2 in the Tammar wallaby (Macropus eugenii)

Alsemgeest, Jenifer; Old, Julie M.; Young, Lauren J.

doi:10.1016/j.vetimm.2013.06.009

Cited by 8 publications

(3 citation statements)

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“…Different splicers of IL6 have been observed in mammals, such as human [30][31][32][33][34], mouse [35], and wallaby (Macropus eugenii) [36]. In human tissues and tumor cell lines, alternative spliced deletion has been observed for exon 2 and/or exon 4 [30][31][32][33][34].…”

Section: Discussionmentioning

confidence: 99%

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A New IL6 Isoform in Chinese Soft-Shelled Turtle (Pelodiscus sinesis) Discovered: Its Regulation during Cold Stress and Infection

Zhang

Tian

Song

et al. 2020

Biology

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The Chinese soft-shelled turtle (Pelodiscus sinesis) is a widely cultured commercial species in East and Southeast Asian countries. The turtles frequently suffer from acute cold stress during farming in China. Stress-induced factor such as Interleukin-6 (IL6) is a multifunctional molecule that plays important roles in innate and adaptive immune response. In the present study, we found that the turtle possessed two IL6 transcripts, where one IL6 transcript contained a signal peptide sequence (psIL6), while the other IL6 transcript (psIL6ns) possessed no such signal peptide gene. To test any differential expression of the two isoforms during temperature and microbial stress, turtles were adapted to optimal environmental water temperature (25 °C), stressed by acute cooling for 24 h, followed with the challenge of Aeromonas hydrophila (1.8 × 108 CFU) or Staphylococcus aureus (5.8 × 108 CFU). Gene characterization revealed that psIL6ns, a splicer without codons encoding a signal peptide and identical to the one predicted from genomic sequence, and psIL6, a splicer with codons encoding a signal peptide, were both present. Inducible expression was documented in primary spleen cells stimulated with ConA and poly I: C. The splenic and intestinal expression of psIL6ns and psIL6 was increased in response to temperature stress and bacterial infection.

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Section: Discussionmentioning

confidence: 99%

“…In mice placenta and in stimulated spleen, both whole and partial exon deletion during RNA splicing have been identified [35]. In wallaby, an alternative splicing variant lacking whole exon 2 was found [36]. In these animals, the loss of exon 2 resulted in a lack of signal peptide.…”

Section: Discussionmentioning

confidence: 99%

A New IL6 Isoform in Chinese Soft-Shelled Turtle (Pelodiscus sinesis) Discovered: Its Regulation during Cold Stress and Infection

Zhang

Tian

Song

et al. 2020

Biology

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“…The publication of the tammar wallaby genome [13] has reinforced this species’ role as a key model marsupial and it is increasingly used in genetic, genomic and other studies [e.g., 1,27,34,35–38]. However, to maximise its usefulness as a model organism there is also a need to assess the overall distribution of genetic diversity within the tammar wallaby and the degree of differentiation amongst populations.…”

Section: Introductionmentioning

confidence: 99%

Extensive genetic differentiation detected within a model marsupial, the tammar wallaby (Notamacropus eugenii)

et al. 2017

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The tammar wallaby (Notamacropus eugenii) is one of the most intensively studied of all macropodids and was the first Australasian marsupial to have its genome sequenced. However, comparatively little is known about genetic diversity and differentiation amongst the morphologically distinct allopatric populations of tammar wallabies found in Western (WA) and South Australia (SA). Here we compare autosomal and Y-linked microsatellite genotypes, as well as sequence data (~600 bp) from the mitochondrial DNA (mtDNA) control region (CR) in tammar wallabies from across its distribution. Levels of diversity at autosomal microsatellite loci were typically high in the WA mainland and Kangaroo Island (SA) populations (A = 8.9–10.6; He = 0.77–0.78) but significantly reduced in other endemic island populations (A = 3.8–4.1; He = 0.41–0.48). Autosomal and Y-linked microsatellite loci revealed a pattern of significant differentiation amongst populations, especially between SA and WA. The Kangaroo Island and introduced New Zealand population showed limited differentiation. Multiple divergent mtDNA CR haplotypes were identified within both SA and WA populations. The CR haplotypes of tammar wallabies from SA and WA show reciprocal monophyly and are highly divergent (14.5%), with levels of sequence divergence more typical of different species. Within WA tammar wallabies, island populations each have unique clusters of highly related CR haplotypes and each is most closely related to different WA mainland haplotypes. Y-linked microsatellite haplotypes show a similar pattern of divergence although levels of diversity are lower. In light of these differences, we suggest that two subspecies of tammar wallaby be recognized; Notamacropus eugenii eugenii in SA and N. eugenii derbianus in WA. The extensive neutral genetic diversity and inter-population differentiation identified within tammar wallabies should further increase the species value and usefulness as a model organism.

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The Immune System of Monotremes and Marsupials

Peel

Belov

2016

Encyclopedia of Immunobiology

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Molecular characterisation and expression of Interleukin-6 and Interleukin-6Δ2 in the Tammar wallaby (Macropus eugenii)

Cited by 8 publications

References 47 publications

A New IL6 Isoform in Chinese Soft-Shelled Turtle (Pelodiscus sinesis) Discovered: Its Regulation during Cold Stress and Infection

A New IL6 Isoform in Chinese Soft-Shelled Turtle (Pelodiscus sinesis) Discovered: Its Regulation during Cold Stress and Infection

Extensive genetic differentiation detected within a model marsupial, the tammar wallaby (Notamacropus eugenii)

The Immune System of Monotremes and Marsupials

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