Molecular Chaperones in Lactic Acid Bacteria: Physiological Consequences and Biochemical Properties

Sugimoto, Shinya; Mahin, Abdullah‐Al; Sonomoto, Kenji

doi:10.1263/jbb.106.324

Cited by 60 publications

(42 citation statements)

References 124 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Some Clp ATPases are able to interact with ClpP, while others function as independent chaperones. ClpP-interacting Clp ATPases, which in LAB typically include the ClpX, ClpC, and ClpE proteins, can be distinguished by the presence of a ClpP recognition tripeptide (302). There are some distinctions between the catalogs of Clp ATPases in Gram-negative and Gram-positive bacteria, but ClpP and ClpX appear to be widely conserved.…”

Section: Treating Damaged Macromoleculesmentioning

confidence: 99%

“…There are some distinctions between the catalogs of Clp ATPases in Gram-negative and Gram-positive bacteria, but ClpP and ClpX appear to be widely conserved. ClpE is absent in E. coli but present in many species of LAB, in conjunction with ClpC, ClpL, and ClpB (302). The ClpL and ClpB proteins lack the ClpP recognition tripeptide and are thus considered to be unable to interact with ClpP.…”

Section: Treating Damaged Macromoleculesmentioning

confidence: 99%

“…Furthermore, sHSPs appear to play an important role in maintaining membrane integrity, especially under stress conditions (332). The sHSP family is widely conserved, and its members have been identified in several LAB (302). In O. oeni, an sHSP named Lo18 was identified as a major stress protein whose synthesis is greatly increased under various conditions, including ethanol shock (333).…”

Section: Treating Damaged Macromoleculesmentioning

confidence: 99%

See 2 more Smart Citations

Stress Physiology of Lactic Acid Bacteria

Papadimitriou

Alegría

Bron

et al. 2016

Microbiol Mol Biol Rev

508

404

View full text Add to dashboard Cite

SUMMARYLactic acid bacteria (LAB) are important starter, commensal, or pathogenic microorganisms. The stress physiology of LAB has been studied in depth for over 2 decades, fueled mostly by the technological implications of LAB robustness in the food industry. Survival of probiotic LAB in the host and the potential relatedness of LAB virulence to their stress resilience have intensified interest in the field. Thus, a wealth of information concerning stress responses exists today for strains as diverse as starter (e.g.,Lactococcus lactis), probiotic (e.g., severalLactobacillusspp.), and pathogenic (e.g.,EnterococcusandStreptococcusspp.) LAB. Here we present the state of the art for LAB stress behavior. We describe the multitude of stresses that LAB are confronted with, and we present the experimental context used to study the stress responses of LAB, focusing on adaptation, habituation, and cross-protection as well as on self-induced multistress resistance in stationary phase, biofilms, and dormancy. We also consider stress responses at the population and single-cell levels. Subsequently, we concentrate on the stress defense mechanisms that have been reported to date, grouping them according to their direct participation in preserving cell energy, defending macromolecules, and protecting the cell envelope. Stress-induced responses of probiotic LAB and commensal/pathogenic LAB are highlighted separately due to the complexity of the peculiar multistress conditions to which these bacteria are subjected in their hosts. Induction of prophages under environmental stresses is then discussed. Finally, we present systems-based strategies to characterize the “stressome” of LAB and to engineer new food-related and probiotic LAB with improved stress tolerance.

show abstract

Section: Treating Damaged Macromoleculesmentioning

confidence: 99%

Section: Treating Damaged Macromoleculesmentioning

confidence: 99%

Section: Treating Damaged Macromoleculesmentioning

confidence: 99%

See 1 more Smart Citation

Stress Physiology of Lactic Acid Bacteria

Papadimitriou

Alegría

Bron

et al. 2016

Microbiol Mol Biol Rev

508

404

View full text Add to dashboard Cite

show abstract

“…Currently, with the rapid development of biotechnology, more and more molecular technology has been applied in genetic diversity research in LAB. DNA-based techniques, such as DNA-DNA hybridization analysis, rRNA homology analysis, plasmid profi ling and randomly amplifi ed polymorphic DNA analysis have been introduced to characterize LAB (Catzeddu et al, 2006;Mohania et al, 2008;Sugimoto et al, 2008;. In our research, we used physiological and molecular methods together.…”

mentioning

confidence: 99%

Identification and characterization of the dominant lactic acid bacteria from kurut: The naturally fermented yak milk in Qinghai, China

Sun

Liu

Wei

et al. 2010

J. Gen. Appl. Microbiol.

View full text Add to dashboard Cite

Forty-three samples of kurut, a kind of traditional naturally-fermented yak milk, were collected in Qinghai, China. One hundred and forty-eight lactic acid bacterial (LAB) strains were isolated and identifi ed from the kurut samples according to phenotypic characterization and 16S rRNA gene sequence analysis. Among them, 52 isolates belonged to the Lactobacillus strains. Ninety-six isolates were resolved to coccoid LAB. The results showed that these isolates belonged to fi ve genera and thirteen different species and subspecies. Moreover, Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus were the predominant population in these samples. This paper systematically studied the composition of LAB in kurut, which may provide raw data for further study involving probiotics strain selection and starter culture design for industrialization production of traditional fermented milk in the future. lactic acid bacteria (LAB). The presence of fermentative LAB is crucial to the intrinsic properties of fermented dairy products (Ehrmann et al., 2002;Tamine and Robinson, 1988). LAB have a long history of safe use, especially in the dairy industry. They play a major role in the production of fermented food, such as the key fl avor, texture and preservative qualities (Ahmad and Irene, 2007;Atrih et al., 2001;Messens and Vuyst, 2002). Recently, some of them have been completely sequenced (Altermann et al., 2005;Guchte et al., 2006;Pridmore et al., 2004). Therefore, comparative genomic analysis can help us to understand the genetic and biological diversity in LAB (Makarova and Koonin, 2007;Makarova et al., 2006;Morita et al., 2008). Key Words-identifiAs yet, information on microbiological composition of kurut is scant. Thus, it is necessary to select LAB strains with benefi cial function and stabile fermentation properties from the traditional fermented milk products, and then use them to design the starter cultures for commercial production. Based on this point, this study was undertaken to enumerate, isolate, identify and characterize the dominant microorganisms from kurut samples obtained from Qinghai, using the conventional method and 16S rRNA sequence analyses. Materials and MethodsSample collection. A total of 43 samples of kurut were collected from scattered households in Qinghai Province of China with temperature ranging from 10.8 C to 39.3 C. At each location, approximately 500 ml kurut were taken aseptically into sterile glass bottles after through mixing in the bulk kurut container. The pH values of these samples were determined at the sampling site using a calibrated portable pH-meter (pH100, Extech, USA). The kurut was collected within 15 min at ambient temperatures, and kept on ice during a 2 h transport. The operation for collection of all kurut samples was completed within 48 h. The microbiological analysis was carried out immediately after the samples arrived at the laboratory.Reference Enumeration of microorganisms. The 10 1 dilution was made by diluting 1 ml of kurut within 9 ml of physiol...

show abstract

“…The role of those chaperones, like the P. gingivalis HSP90 homologue HtpG, in immune response dynamics has become an area of intense investigation (12). It has also been suggested that chaperones are probably important in the interaction between the host and the commensal microbial flora (17,22,46), functions important in the establishment and perpetuation of chronic inflammatory diseases. In addition, HtpG induces a strong humoral response that may have consequences in the pathogenesis of periodontitis (27).…”

mentioning

confidence: 99%

Immunoglobulin G (IgG) Class, but Not IgA or IgM, Antibodies to Peptides of thePorphyromonas gingivalisChaperone HtpG Predict Health in Subjects with Periodontitis by a Fluorescence Enzyme-Linked Immunosorbent Assay

Sweier¹,

Shelburne²,

Giannobile³

et al. 2009

Clin Vaccine Immunol

View full text Add to dashboard Cite

Chaperones are molecules found in all cells and are critical in stabilization of synthesized proteins, in repair/removal of defective proteins, and as immunodominant antigens in innate and adaptive immunity. Subjects with gingivitis colonized by the oral pathogen Porphyromonas gingivalis previously demonstrated levels of anti-human chaperone Hsp90 that were highest in individuals with the best oral health. We hypothesized that similar antibodies to pathogen chaperones might be protective in periodontitis. This study examined the relationship between antibodies to P. gingivalis HtpG and clinical statuses of healthy and periodontitissusceptible subjects. We measured the humoral responses (immunoglobulin G [IgG], IgA, and IgM) to peptides of a unique insert (P18) found in Bacteroidaceae HtpG by using a high-throughput, quantitative fluorescence enzyme-linked immunosorbent assay. Indeed, higher levels of IgG class anti-P. gingivalis HtpG P18 peptide (P < 0.05) and P18␣, consisting of the N-terminal 16 amino acids of P18 (P < 0.05), were associated with better oral health; these results were opposite of those found with anti-P. gingivalis whole-cell antibodies and levels of the bacterium in the subgingival biofilm. When we examined the same sera for IgA and IgM class antibodies, we found no significant relationship to subject clinical status. The relationship between anti-P18 levels and clinical populations and individual subjects was found to be improved when we normalized the anti-P18␣ values to those for anti-P18␥ (the central 16 amino acids of P18). That same ratio correlated with the improvement in tissue attachment gain after treatment (P < 0.05). We suggest that anti-P. gingivalis HtpG P18␣ antibodies are protective in periodontal disease and may have prognostic value for guidance of individual patient treatment.

show abstract

Molecular Chaperones in Lactic Acid Bacteria: Physiological Consequences and Biochemical Properties

Cited by 60 publications

References 124 publications

Stress Physiology of Lactic Acid Bacteria

Stress Physiology of Lactic Acid Bacteria

Identification and characterization of the dominant lactic acid bacteria from kurut: The naturally fermented yak milk in Qinghai, China

Immunoglobulin G (IgG) Class, but Not IgA or IgM, Antibodies to Peptides of thePorphyromonas gingivalisChaperone HtpG Predict Health in Subjects with Periodontitis by a Fluorescence Enzyme-Linked Immunosorbent Assay

Contact Info

Product

Resources

About