Molecular biological characterization of biphenyl-degrading bacteria and identification of the biphenyl 2,3-Dioxygenase α-subunit genes

Шумкова, Е. С.; Егорова, Д. О.; Korsakova, E. S.; Dorofeeva, Lubov V.; Плотникова, Е. Г.

doi:10.1134/s0026261714010135

Cited by 4 publications

(1 citation statement)

References 25 publications

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“…X80625]), physiological, and biochemical features analysis ( 1 ). Strain P25 was capable of utilizing a broad range of aromatic compounds (biphenyl, phenol, toluene, naphthalene, salicylate, gentisate, ortho -phthalate, benzoate, 3-hydroxybenzoate, 4-hydroxybenzoate, and 3,4-dihydroxybenzoate), as well as their substituted derivatives (chlorinated biphenyls and chlorobenzoates, para -methylbenzoate, and 2,4-dichlorophenoxyacetate) as a sole carbon and energy source ( 2 – 4 ). R. ruber P25 is an active destructor of polychlorinated biphenyls, which are toxic and persistent organic pollutants ( 5 ).…”

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequence of Rhodococcus ruber Strain P25, an Active Polychlorinated Biphenyl Degrader

et al. 2015

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Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequence of Rhodococcus ruber Strain P25, an Active Polychlorinated Biphenyl Degrader

et al. 2015

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Chloromuconolactone dehalogenase ClcF of actinobacteria

Solyanikova¹,

Плотникова²,

Шумкова³

et al. 2014

Journal of Environmental Science and Health, Part B

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This work investigated the distribution of the clcF gene in actinobacteria isolated from different ecotopes. The gene encodes chloromuconolactone dehalogenase (CMLD) ClcF, the enzyme found to date in only one representative of Gram-positive bacteria, Rhodococcus opacus 1CP, adapted to 2-chlorophenol (2CP). Using primers specific to the clcF gene, from the DNA matrix of rhodococcal strains closely related to species Rhodococcus wratislaviensis (P1, P12, P13, P20, G10, KT112, KT723, BO1) we obtained PCR products whose nucleotide sequences were 100% identical to that of the clcF gene from strain R. opacus 1CP. CMLDs isolated from the biomass of strains Rhodococcus spp. G10 and P1 grown on 2CP did not differ by their subunit molecular mass deduced from the known amino acid sequence of the clcF gene from the ClcF of strain R. opacus 1CP. Matrix-assisted laser dissociation/ionization time-of-flight mass spectrometry showed the presence of a peak with m/z 11,194-11,196 Da both in whole cells and in protein solutions with a ClcF activity. Thus, we have first time shown the distribution of ClcF among actinobacteria isolated from geographically distant habitats.

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Polymorphism of the bphA genes in bacteria destructing biphenyl/chlorinated biphenils

et al. 2015

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Molecular biological characterization of biphenyl-degrading bacteria and identification of the biphenyl 2,3-Dioxygenase α-subunit genes

Cited by 4 publications

References 25 publications

Draft Genome Sequence of Rhodococcus ruber Strain P25, an Active Polychlorinated Biphenyl Degrader

Draft Genome Sequence of Rhodococcus ruber Strain P25, an Active Polychlorinated Biphenyl Degrader

Chloromuconolactone dehalogenase ClcF of actinobacteria

Polymorphism of the bphA genes in bacteria destructing biphenyl/chlorinated biphenils

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