Molecular Biological Characterization of a Highly Leukaemogenic Virus Isolated from the Mouse. III. Identity with Mouse Mammary Tumour Virus

Abstract

SUMMARYA highly leukaemogenic virus isolate (DMBA-LV) endogenous to the CFW/D mouse has been found to contain two viral genomes. One was closely related to the type B milk-borne mouse mammary tumour virus (MMTV) and present in tenfold excess over a type C viral genome which was only partially related to xenotropic and polytropic isolates from the CFW/D mouse as well as to the ecotropic Moloney routine leukaemia virus isolate. The thymic lymphoma cell line that produced DMBA-LV expressed high levels of MMTV viral RNA (35S and the 24S envelope mRNA). Both the virus and the virus-producing cell line expressed multiple species of type C viral RNA. Similar species of type C viral RNA were also associated with non-infectious, non-leukaemogenic viral particles present in both normal lymphoid cells and in a MMTV-free thymic lymphoma cell line established from a second chemical carcinogen-induced tumour.

Factors controlling the expression of mouse mammary tumour virus

“…In certain inbred strains of mice T-cell lymphomagenesis is directly correlated with the presence and expression of newly acquired multiple copies of MMTV (Ball & McCarter, 1979;Michalides et al, 1982;Ball et al, 1983;Dudley & Risser, 1984;Ball & Dekaban, 1987 Wagenaar, 1986;Lee et al, 1987;Ball et al, 1988) (Fig. 1).…”

Molecular Biological Characterization of a Highly Leukaemogenic Virus Isolated from the Mouse. IV. Viral Proteins

“…The origin and properties of these cell lines have been described (Ball, 1979;Ball & McCarter, 1979). The DMT-10 cell line which produces a non-infectious type C retrovirus has been described (Ball et aL, 1983).…”

“…The origin and properties of these cell lines have been described (Ball, 1979;Ball & McCarter, 1979). The DMT-10 cell line which produces a non-infectious type C retrovirus has been described (Ball et aL, 1983). The 3T3 FL cells chronically infected with Moloney murine leukaemia virus (MoMuLV/ICB) and the Hix cell line chronically infected with a recombinant type C retrovirus were kindly provided by Dr P. J. Fischinger.…”

“…In a preceding paper (Ball et at., 1983) we showed, using molecular probes, that DMBA-LV consisted of two unrelated retrovirus genomes: an intact type B retrovirus highly related to the 0022-1317/84/0000-5891 $02.00© 1984 SGM exogenous milk-borne MMTV isolate and a biologically defective type C genome. In this paper we report that DMBA-LV contained all the proteins corresponding to those characteristic of type B retroviruses and that the processing of these proteins in cells producing the virus proceeds normally.…”

Expression and immunogenicity of the entire human T cell leukaemia virus type I envelope protein produced in a baculovirus system

“…Three different inbred mouse strains, BALB/c (Charles River), C57BL/6 (Charles River) and CFW/D (Ball & McCarter, 1979) were immunized at 6 to 8 weeks of age by intraperitoneal injection. Two different forms of HTLV-I envelope protein immunogens were studied.…”