Molecular biological and histological effects of Er:YAG laser irradiation on tooth movement

Tsuka, Yuji; Kunimatsu, Ryo; Gunji, Hidemi; Nakajima, Kengo; Hiraki, Tomoka; Nakatani, Ayaka; Tanimoto, Kotaro

doi:10.2334/josnusd.17-0472

Cited by 3 publications

(3 citation statements)

References 25 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This study led to a detailed in vitro elucidation of the effects of Er: YAG laser irradiation on bone metabolism during experimental tooth movement as performed in our previous study. 17 In addition, the Er: YAG laser is frequently used in clinical practice for periimplantitis, which has become a problem in recent years, and it will help to establish evidence for these treatments.…”

Section: Discussionmentioning

confidence: 99%

“…Irradiation with a diode laser 15 or Nd: YAG laser 16 has been shown to lead to the proliferation of osteoblasts. In addition, the application of Er: YAG, 17 Nd: YAG, 18 and diode laser irradiation 19 during experimental tooth movement has been shown to accelerate tooth movement and lead to changes in bone metabolism. As described above, various lasers have been suggested to promote calcification, bone formation, and bone healing at the level of cell and animal experiments, that is, the possibility of promoting bone metabolism.…”

Section: Introductionmentioning

confidence: 99%

“…Also, to investigate in more detail the effect of Er:YAG laser irradiation on the periodontal tissue, which we have previously studied. 17 Given this background, the present in vitro study aimed to investigate the effects of Er: YAG laser irradiation on bone metabolism using human osteoblast-like cells.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of Er: YAG Laser Irradiation on Bone Metabolism-Related Factors Using Cultured Human Osteoblasts

et al. 2023

View full text Add to dashboard Cite

Introduction: A variety of laser treatments have been applied in numerous medical fields. In dentistry, laser treatments are used for caries, root canals, and periodontal disease, as well as surgical resection. Numerous reports have recently been published on the use of lasers for bone regeneration. If laser irradiation is found to promote the activation of bone metabolism, it might also be effective for periodontal treatment, peri-implantitis, and bone regeneration. Therefore, the present in vitro study aimed to elucidate the mechanisms underlying the effects of erbium-doped yttrium aluminum garnet (Er: YAG) laser irradiation on the bone using osteoblast-like cells. Methods: Osteoblast-like Saos 2 cells (5.0×104 cells) were seeded in 24-well plates. 24 hours after being seeded, the cells were subjected to 0.3 W, 0.6 W, and 2.0 W Er: YAG laser irradiation and then allowed to recover for 48 hours. The expression levels of bone metabolism-related factors alkaline phosphatase (ALP), bone sialoprotein (BSP), and osteoprotegerin (OPG) were then evaluated using reverse transcription–quantitative polymerase chain reaction and western blot analyses. Results: Saos 2 cells subjected to Er: YAG laser irradiation at 0.3 W, 0.6 W, and 2.0 W showed normal growth. When the Er: YAG laser irradiation and control groups were compared after 48 hours, increases were observed in ALP, BSP, and OPG gene and protein expression in the 2.0 W group. Similar results were obtained in the western blot analysis. Conclusion: These findings suggest that the Er: YAG laser irradiation of osteoblast-like cells is effective for activating bone metabolism factors.

show abstract

Section: Discussionmentioning

confidence: 99%