Molecular Beacon Probes of Oligonucleotides Photodamaged by Psoralen

Shire, Zahra; Loppnow, Glen R.

doi:10.1111/j.1751-1097.2012.01109.x

Cited by 7 publications

(2 citation statements)

References 28 publications

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“…This light-activated insecticide is expected to become an effective alternative for pest control due to its lower toxicity to mammals, higher insecticidal efficiency, and environmental friendliness [ 11 , 12 , 13 ]. Previous studies showed that furanocoumarins activated by light could react with bases on DNA double-strands to form double-crosses and destroy DNA replication, inhibit the growth of a variety of cancer cells, and become a potential drug for treating cancer [ 14 ]. The effect of furanocoumarins on photoactivated pesticides was first shown by Berenbaum.…”

Section: Introductionmentioning

confidence: 99%

Potential Biochemical Pesticide—Synthesis of Neofuranocoumarin and Inhibition the Proliferation of Spodoptera frugiperda Cells through Activating the Mitochondrial Pathway

Shao

Zhang

Qian

et al. 2022

Toxins

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Furanocoumarins, the secondary metabolites of plants, are considered to be natural insecticides and fungicides because they prevent the invasion of plant pathogenic microorganisms and the predation of herbivorous insects. In this study, novel 2-arylfuranocoumarin derivatives were designed to synthesize by condensation, esterification, bromination, and Wittig reaction. The results showed an excellent photosensitive activity of 2-thiophenylfuranocoumarin (I34). Cell Counting Kit-8 detected that I34 could inhibit the proliferation of Spodoptera frugiperda (Sf9) cells in a time- and concentration-dependent manner under ultraviolet A (UV-A) light for 3 min. The inverted microscope revealed that cells treated with I34 swelled, the membrane was ruptured, and apoptotic bodies appeared. The flow cytometry detected that I34 could induce apoptosis of Sf9 cells, increase the level of intracellular reactive oxygen species (ROS), decrease the mitochondrial membrane potential, and block cell cycle arrest in the G2/M phase. Transmission electron microscopy detected cell mitochondrial cristae damage, matrix degradation, and mitochondrial vacuolation. Further enzyme activity detection revealed that the enzyme activities of apoptosis-related proteins caspase-3 and caspase-9 increased significantly (P < 0.05). Finally, Western blotting analysis detected that the phosphorylation level of Akt and Bad and the expression of the apoptosis inhibitor protein Bcl-XL were inhibited, cleaved-PARP and P53 were increased, and cytochrome C was released from the mitochondria into the cytoplasm. Moreover, under UV-A irradiation, I34 promoted the increase in ROS in Sf9 cells, activated the mitochondrial apoptotic signal transduction pathway, and finally, inhibited cell proliferation. Thus, novel furanocoumarins exhibit a potential application prospect as a biochemical pesticide.

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Section: Introductionmentioning

confidence: 99%

Potential Biochemical Pesticide—Synthesis of Neofuranocoumarin and Inhibition the Proliferation of Spodoptera frugiperda Cells through Activating the Mitochondrial Pathway

Shao

Zhang

Qian

et al. 2022

Toxins

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“…Thus, while LC‐MS/MS is necessary to determine a given population of adducts in addition to total binding level, DNA digestion to nucleosides and postdigestion purification make the procedure time‐consuming. Shire and Loppnow showed that oligonucleotides may be desorbed in matrix‐assisted laser desorption ionization (MALDI) by using the appropriate matrix and charge mode . In this report, we also tested whether surface‐enhanced laser desorption ionization (SELDI‐TOF) technology , a variant of MALDI which combines chromatography with mass spectrometry through the use of affinity surfaces, may be used for psoralen‐DNA adduct detection.…”

Section: Introductionmentioning

confidence: 99%

UVA and UVB‐Induced 8‐Methoxypsoralen Photoadducts and a Novel Method for their Detection by Surface‐Enhanced Laser Desorption Ionization Time‐of‐Flight Mass Spectrometry (SELDI‐TOF MS)

Buhimschi

Gasparro

2013

Photochem & Photobiology

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UVA-activated psoralens are used to treat hyperproliferative skin conditions due to their ability to form DNA photoadducts, which impair cellular processes and may lead to cell death. Although UVA (320-400 nm) is more commonly used clinically, studies have shown that UVB (280-320 nm) activation of psoralen can also be effective. However, there has been no characterization of UVB-induced adduct formation in DNA alone. As psoralen derivatives have a greater extinction coefficient in the UVB region (11 800 cm(-1) M(-1) at 300 nm) compared with the UVA region (2016 cm(-1) M(-1) at 365 nm), a greater extent of adduct formation is expected. SELDI-TOF, a proteomic technique that combines chromatography with mass spectrometry, was used to detect photoadduct formation in an alternating A-T oligonucleotide. 8-Methoxypsoralen (8-MOP) and DNA solutions were irradiated with either UVA or UVB. An adduct peak was obtained with SELDI-TOF. For UVB-activated 8-MOP, the extent of adducts was three times greater than for UVA. HPLC ESI-MS analysis showed that UVB irradiation yielded high levels of 3,4-monoadducts (78% of total adducts). UVA was more effective than UVB at conversion of 4',5'-monoadducts to crosslinks (17% vs 4%, respectively). This report presents a method for comparing DNA binding efficiencies of interstrand crosslink inducing agents.

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Inexpensive bioluminescent genosensor for sensitive determination of DNA damage induced by some commonly used sunscreens

El-Yazbi

Khalil

Belal

et al. 2022

Analytical Biochemistry

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Molecular Beacon Probes of Oligonucleotides Photodamaged by Psoralen

Cited by 7 publications

References 28 publications

Potential Biochemical Pesticide—Synthesis of Neofuranocoumarin and Inhibition the Proliferation of Spodoptera frugiperda Cells through Activating the Mitochondrial Pathway

Potential Biochemical Pesticide—Synthesis of Neofuranocoumarin and Inhibition the Proliferation of Spodoptera frugiperda Cells through Activating the Mitochondrial Pathway

UVA and UVB‐Induced 8‐Methoxypsoralen Photoadducts and a Novel Method for their Detection by Surface‐Enhanced Laser Desorption Ionization Time‐of‐Flight Mass Spectrometry (SELDI‐TOF MS)

Inexpensive bioluminescent genosensor for sensitive determination of DNA damage induced by some commonly used sunscreens

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