Inexpensive bioluminescent genosensor for sensitive determination of DNA damage induced by some commonly used sunscreens

Triggering Breast Cancer Apoptosis via Cyclin-Dependent Kinase Inhibition and DNA Damage by Novel Pyrimidinone and 1,2,4-Triazolo[4,3-a]pyrimidinone Derivatives

“…Many fluorescent biosensors are reported in literature for assessing DNA interaction with small molecules. − Herein, terbium­(III) chloride (Tb) luminescent biosensor was selected as it has superior sensitivity, selectivity over many other reported biosensors, in addition to being inexpensive with simple mix-and-read procedure . Tb3+ is a trivalent lanthanide with low fluorescence in aqueous solutions. , Upon addition of the Tb biosensor to the DNA solution, it binds to DNA. The binding sites of Tb in single- and double-stranded DNA are different.…”

“… 44 Tb3+ is a trivalent lanthanide with low fluorescence in aqueous solutions. 30 , 31 Upon addition of the Tb biosensor to the DNA solution, it binds to DNA. The binding sites of Tb in single- and double-stranded DNA are different.…”

“…With that, the target series were screened for their potential cytotoxic activities against MCF-7 and MDA-MB237 breast cancer cells as well as Wi-38 normal human fibroblasts by MTT assay compared to the DNA intercalator doxorubicin. The most promising cytotoxic derivatives regarding potency and selectivity against breast cancer cell line over normal cells were then evaluated for their potential DNA-binding affinity utilizing luminescent terbium­(III) chloride (Tb3+) biosensor , compared to doxorubicin, followed by in vitro assessment of their CDK-2 inhibitory activities. , Breast cancer apoptosis studies were performed for cell cycle analysis and quantitation of apoptotic induction capacity of the hit compounds utilizing flow cytometry. Finally, the active derivatives–receptors binding analyses were simulated via docking and molecular dynamics to enrich the information required for concluding the structural determinants of activities.…”

New imidazole-2-thiones linked to acenaphythylenone as dual DNA intercalators and topoisomerase II inhibitors: structural optimization, docking, and apoptosis studies

“…With dsDNA, Tb 3+ ions are electrostatically attracted to the negatively charged phosphate backbone of the DNA. On the other hand, with ssDNA, it coordinates with the lone pairs of electrons of the free nucleobases, where upon DNA excitation, energy is transferred to the Tb biosensor causing significant enhancement of its luminescence 47 . Therefore, the assessed compound damages dsDNA, ssDNA sites would be formed opposite to the site of damage and Tb 3+ would directly coordinate to the unpaired nucleobases in this single-stranded site.…”