Molecular basis of seed lipoxygenase null traits in soybean line OX948

Reinprecht, Yarmilla; Luk-Labey, Shun-Yan; Yu, Kangfu; Poysa, Vaino; Rajcan, Istvan; Ablett, G. R.; Pauls, K. Peter

doi:10.1007/s00122-011-1528-5

Cited by 20 publications

(35 citation statements)

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“…The linoleate 9S-lipoxygenase 5-like sequence from soybean (gene ID: 100802887) has an intron 1 size of 1,956 bp, which is comparable with that of AhLOX7 . Based on soybean lipoxygenase gene information [ 38 , 39 ], the corresponding locations of AhLOX7 conserved histidine residues and fatty acid, iron, and oxygen binding sites were located in exons 7 to 9 (Table 1 ).…”

Section: Resultsmentioning

confidence: 99%

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TILLING by sequencing to identify induced mutations in stress resistance genes of peanut (Arachis hypogaea)

et al. 2015

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BackgroundTargeting Induced Local Lesions in Genomes (TILLING) is a powerful reverse genetics approach for functional genomics studies. We used high-throughput sequencing, combined with a two-dimensional pooling strategy, with either minimum read percentage with non-reference nucleotide or minimum variance multiplier as mutation prediction parameters, to detect genes related to abiotic and biotic stress resistances. In peanut, lipoxygenase genes were reported to be highly induced in mature seeds infected with Aspergillus spp., indicating their importance in plant-fungus interactions. Recent studies showed that phospholipase D (PLD) expression was elevated more quickly in drought sensitive lines than in drought tolerant lines of peanut. A newly discovered lipoxygenase (LOX) gene in peanut, along with two peanut PLD genes from previous publications were selected for TILLING. Additionally, two major allergen genes Ara h 1 and Ara h 2, and fatty acid desaturase AhFAD2, a gene which controls the ratio of oleic to linoleic acid in the seed, were also used in our study. The objectives of this research were to develop a suitable TILLING by sequencing method for this allotetraploid, and use this method to identify mutations induced in stress related genes.ResultsWe screened a peanut root cDNA library and identified three candidate LOX genes. The gene AhLOX7 was selected for TILLING due to its high expression in seeds and roots. By screening 768 M2 lines from the TILLING population, four missense mutations were identified for AhLOX7, three missense mutations were identified for AhPLD, one missense and two silent mutations were identified for Ara h 1.01, three silent and five missense mutations were identified for Ara h 1.02, one missense mutation was identified for AhFAD2B, and one silent mutation was identified for Ara h 2.02. The overall mutation frequency was 1 SNP/1,066 kb. The SNP detection frequency for single copy genes was 1 SNP/344 kb and 1 SNP/3,028 kb for multiple copy genes.ConclusionsOur TILLING by sequencing approach is efficient to identify mutations in single and multi-copy genes. The mutations identified in our study can be used to further study gene function and have potential usefulness in breeding programs.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1348-0) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

“…Based on soybean lipoxygenase gene information [ 38 , 39 ], the corresponding locations of AhLOX7 conserved histidine residues and fatty acid, iron, and oxygen binding sites were located in exons 7 to 9 (Table 1 ).…”

Section: Resultsmentioning

confidence: 99%

TILLING by sequencing to identify induced mutations in stress resistance genes of peanut (Arachis hypogaea)

et al. 2015

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“…In the VIR collection, there are samples with lx recessive alleles; adding them to the genome of commercial soybean genotypes will reduce lipoxygenase activity to get products that are less prone to undesirable oxidation. To date, these genes have been sequenced, and molecular markers can be used to speed up the search for the desired genotypes [50].…”

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confidence: 99%

GENETIC SOURCES REQUIRED FOR SOYBEAN BREEDING IN THE CONTEXT OF NEW BIOTECHNOLOGies (review)

Vishnyakova¹,

Сеферова²,

Samsonova³

2017

S-h. biol.

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Soybean is a strategic crop of multipurpose use. Production and consumption of soybeans are increasing year by year, with new uses appeared. Soybean can become one of the key plants in bioeconomics. Food, fodder, technical, medical and pharmaceutical use of soybeans is diversified and requires specialized varieties with the target traits. This poses new challenges for breeders and, accordingly, for holders of germplasm collections that supply source material for breeding. VIR soybean collection for many years serves as a genetic source for breeding. Based on long-term phenotyping, the accessions are systematized by a number of traits. Rapid development of new molecular technologies, e.g. marker-assisted selection (MAS) and genomic breeding are targeted to optimize both creation of new varieties and searching for the necessary genotypes. A number of agronomically important quantitative trait loci (QTL) have been found for soybean (Y.

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“…Transcript levels of KTI3, CPI2, and NES1 increased to the maximum levels in ED059, indicating that PI and monoterpene may both contribute to conferring defense against insects. The biosynthesis of JA is essential for the production of induced defense responses to herbivores in many plants [37], and AOC3 is an important enzyme that promotes JA biosynthesis [38][39][40][41]. We found that H. armigera induced higher levels of JA and JA biosynthesis enzyme AOC3 gene expression in ED059 than in Tianlong 2.…”

Section: Discussionmentioning

confidence: 78%

Laboratory testing and molecular analysis of the resistance of wild and cultivated soybeans to cotton bollworm, Helicoverpa armigera (Hübner)

et al. 2015

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Identifying a superior soybean variety with high defoliator resistance is important to avoid yield loss. Cotton bollworm (Helicoverpa armigera Hübner) is one of the major defoliators of soybean (Glycine max [L.] Merr.) worldwide. In this study, we evaluated the effect of H. armigera larvae on ED059, a wild soybean (Glycine soja Sieb. et Zucc.), and three cultivated soybean varieties: Tianlong 2, PI 535807, and PI 533604, in choice and no-choice assays. The percentage of ED059 leaflets consumed by H. armigera was lower than that of the three cultivated soybeans. Larvae that fed on ED059 exhibited low weight gain and high mortality rate.Waldbauer nutritional indices suggested that ED059 reduced the growth, consumption, and frass production of H. armigera larvae. Larvae that fed on ED059 showed lower efficiency of conversion of ingested and of digested food than those that fed on Tianlong 2 and PI 533604. However, they showed statistically similar consumption index and approximate digestibility compared with those fed on the three cultivated soybeans. Quantitative real-time PCR analysis revealed that 24 h after insect attack, ED059 had higher transcript levels of Kunitz trypsin inhibitor 3, Cysteine proteinase inhibitor 2, and Nerolidol synthase 1 but a lower transcript level of Pathogenesis-related protein 1 than Tianlong 2. The gene expression results were consistent with the presence of higher levels of jasmonic acid (JA) and transcript levels of the JA biosynthesis enzyme allene oxide cyclase 3 in ED059 than in Tianlong 2. Our findings indicate that ED059 is a superior soybean line with strong insect resistance that may be mediated via the JA pathway.

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Molecular basis of seed lipoxygenase null traits in soybean line OX948

Cited by 20 publications

References 46 publications

TILLING by sequencing to identify induced mutations in stress resistance genes of peanut (Arachis hypogaea)

TILLING by sequencing to identify induced mutations in stress resistance genes of peanut (Arachis hypogaea)

GENETIC SOURCES REQUIRED FOR SOYBEAN BREEDING IN THE CONTEXT OF NEW BIOTECHNOLOGies (review)

Laboratory testing and molecular analysis of the resistance of wild and cultivated soybeans to cotton bollworm, Helicoverpa armigera (Hübner)

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