Molecular basis for resistance to ACCase-inhibiting fluazifop in Eleusine indica from Malaysia

San, Thye; Najihah, Mohamed Ghazani; Sahid, Ismail; Chuah, Tse Seng

doi:10.1016/j.pestbp.2014.04.011

Cited by 33 publications

(37 citation statements)

References 34 publications

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“…ha -1 ) and might be considered resistant biotypes, since, normally, susceptible biotypes treated with the recommended field dose die. Similar results have been observed and confirmed for E. indica (Jalaludin et al, 2014;Cha et al, 2014), and Sorghum halepense (Johnsongrass) (Scarabel et al, 2014) treated with fluazifop-P-butyl at the double recommended field rate.…”

Section: Resultssupporting

confidence: 76%

“…No other known mutations were found in the two examined regions of the CT domain. The Trp-2027-Cys mutation has been extensively shown to link to APPs-resistance in several grass weed species Liu et al, 2007;Beckie et al, 2012;Gherekhloo et al, 2012;Kukorelli et al, 2013;Li et al, 2014) and particularly to fluazifop-P-butyl in Goosegrass (Cha et al, 2014;Jalaludin et al, 2014), Sudan grass (Kershner et al, 2012), and Japanese foxtail (Xu et al, 2013). Thus, it is our conclusion that a R. cochinchinensis population from Costa Rica has evolved resistance to fluazifop-P-butyl and the mechanism of resistance is based on an altered target site conferred by the Trp-2027-Cys mutation.…”

Section: Resultsmentioning

confidence: 99%

“…Also, populations from different geographical areas could harbor different mutations (Yu et al, 2007;Liu et al, 2007;Cruz-Hipolito et al, 2011;Délye et al, 2010;Gherekhloo et al, 2012;Marshall et al, 2013;Li et al, 2013Li et al, , 2014Malone et al, 2014;Martins et al, 2014). In addition, the level of resistance depends on the number of resistant alleles in individual plants (Cha et al, 2014;Martins et al, 2014). Cha et al (2014) have demonstrated that an E. indica biotype containing homozygous 2027-Cys allele could endow a higher level of resistance to fluazifop-P-butyl than a mix (heterozygous) Trp-and Cys-2027 allele biotype.…”

Section: Resultsmentioning

confidence: 99%

“…Plants were daily monitored and visually assessed 3 weeks after treatment to determine if they were resistant (survived) or susceptible (dead). Two (2X) to three (3X) times of the recommended fluazifop-P-butyl field dose were frequently used as discriminatory doses between susceptible and resistant plants in several weeds (Scarabel et al, 2014;Jalaludin et al, 2014;Cha et al, 2014). In addition, R. cochinchinensis susceptible plants die when exposed to the recommended field dose (1X) in Costa Rica.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Basis for Resistance to Fluazifop-P-Butyl in Itchgrass (Rottboellia cochinchinensis) from Costa Rica

Castillo-Matamoros¹,

Brenes-Angulo²,

Murillo

et al. 2016

Planta daninha

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Rottboellia cochinchinensis is an annual grass weed species known as itchgrass, or "caminadora" in America´s Spanish speaking countries, and has become a major and troublesome weed in several crops. The application of fluazifop-P-butyl at recommended rates (125 g a.i. ha-1) was observed to be failing to control itchgrass in a field in San José, Upala county, Alajuela province, Costa Rica. Plants from the putative resistant R. cochinchinensis population survived fluazifop-P-butyl when treated with 250 g a.i. ha-1 (2X label rate) at the three- to four-leaf stage under greenhouse conditions. PCR amplification and sequencing of partial carboxyl transferase domain (CT) of the acetyl-CoA carboxylase (ACCase) gene were used to determine the molecular mechanism of resistance. A single non-synonymous point mutation from TGG (susceptible plants) to TGC (putative resistant plants) that leads to a Trp-2027-Cys substitution was found. This Trp-2027-Cys mutation is known to confer resistance to all aryloxyphenoxyproprionate (APP) herbicides to which fluazifop-P-butyl belongs. To the best of our knowledge, this is the first report of fluazifop-P-butyl resistance and a mutation at position 2027 for a Costa Rican R. cochinchinensis population.

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Section: Resultssupporting

confidence: 76%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular Basis for Resistance to Fluazifop-P-Butyl in Itchgrass (Rottboellia cochinchinensis) from Costa Rica

Castillo-Matamoros¹,

Brenes-Angulo²,

Murillo

et al. 2016

Planta daninha

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“…The magnitude of resistance informs us on potential resistance mechanisms. For example, three goosegrass (Eleusine indica L.) populations in Malaysia showed different levels of resistance to fluazifop, an inhibitor of acetyl coenzyme-A carboxylase (ACCase, EC 6.4.1.2) (Cha et al 2014). Two populations had lower resistance levels (R/S 5 62 and 88) attributed to an ACCase mutation, Trp 2027 Cys.…”

Section: ½1mentioning

confidence: 99%

Whole-Plant and Seed Bioassays for Resistance Confirmation

Burgos

2015

Weed sci.

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Much of agriculture-related research today involves weed resistance to herbicides. Resistance evolution is perhaps the strongest driver for the quest for new herbicide targets, novel weed intervention technologies, and the promotion of best management practices for sustainable crop production (Burgos et al., 2006; Norsworthy et al. 2012; Vencill et al. 2012). To date, 222 weedy species collectively have evolved resistance to 150 herbicides representing 21 sites of action (Heap 2014). For decades, scientists have developed numerous protocols for resistance confirmation using seeds, different plant parts, or whole plants. These have been reviewed by Beckie et al. (2000) and Burgos et al. (2013). We draw from these and other sources to present general guidelines for resistance confirmation that students and new researchers can use in planning their experiments. The most immediate questions that stakeholders seek to answer with resistance bioassays include:1.Is the population resistant?2.What is the level of resistance?3.What alternative herbicides can be used?

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Target‐site mutations Ile1781Leu and Ile2041Asn in the ACCase2 gene confer resistance to fluazifop‐p‐butyl and pinoxaden herbicides in a johnsongrass accession from Arkansas, USA

González‐Torralva,

Norsworthy

2024

Plant Direct

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Johnsongrass [Sorghum halepense (L.) Pers.] is a troublesome weed species in different agricultural and non‐agricultural areas. Because of its biology, reproductive system, and seed production, effective management is challenging. An accession with low susceptibility to the acetyl‐CoA carboxylase (ACCase)‐inhibiting herbicides fluazifop‐p‐butyl (fluazifop) and pinoxaden was collected in eastern Arkansas. In this research, the molecular mechanisms responsible for ACCase resistance were investigated. Dose–response experiments showed a resistance factor of 181 and 133 for fluazifop and pinoxaden, respectively. Molecular analysis of both ACCase1 and ACCase2 genes was researched. Nucleotide comparison of ACCase1 between resistant and susceptible accessions showed no single nucleotide polymorphisms. Nonetheless, analysis of ACCase2 in fluazifop‐resistant johnsongrass plants revealed the Ile1781Leu target‐site mutation was dominant (nearly 75%), whereas the majority of pinoxaden‐resistant johnsongrass plants had the Ile2041Asn (60%). Not all sequenced johnsongrass plants displayed a target‐site mutation, suggesting the presence of additional resistance mechanisms. Amplification of ACCase1 and ACCase2 was not responsible for resistance because of the similar values obtained in both resistant and susceptible accessions. Experiments with malathion and NBD‐Cl suggest the presence of herbicide metabolism. Outcomes of this research demonstrated that fluazifop‐ and pinoxaden‐resistant johnsongrass plants displayed a target‐site mutation in ACCase2, but also that non‐target‐site resistance mechanisms would be involved and require a detailed study.

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Molecular basis for resistance to ACCase-inhibiting fluazifop in Eleusine indica from Malaysia

Cited by 33 publications

References 34 publications

Molecular Basis for Resistance to Fluazifop-P-Butyl in Itchgrass (Rottboellia cochinchinensis) from Costa Rica

Molecular Basis for Resistance to Fluazifop-P-Butyl in Itchgrass (Rottboellia cochinchinensis) from Costa Rica

Whole-Plant and Seed Bioassays for Resistance Confirmation

Target‐site mutations Ile1781Leu and Ile2041Asn in the ACCase2 gene confer resistance to fluazifop‐p‐butyl and pinoxaden herbicides in a johnsongrass accession from Arkansas, USA

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