Molecular Authentication of Alisma orientale by PCR-RFLP and ARMS

Abstract

As a widely used medicinal plant, Alisma orientale is always a possible target for fraudulent labeling. The internal transcribed spacer (ITS) regions of nuclear ribosomal DNA (nrDNA) of the six species of genus Alisma were sequenced, and two variant sites were found to be specific for A. orientale. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis and amplification refractory mutation system (ARMS) analysis were applied to the ITS region for the identification of A. orientale. A restriction site for PSTI useful for PCR-RFLP analysis was detected and a pair of diagnostic primers DFZX-JB02S and DFZX-JB02X were designed for ARMS.

SNP, ARMS and SSH Authentication of Medicinal Dendrobium officinale KIMURA et MIGO and Application for Identification of Fengdou Drugs

“…5,14,26) In the present study, six pairs of diagnostic primers were designed to amplify rDNA ITS region of six wild populations and "Fengdou" drugs. GSG, FSC, and YWS populations that compatible of processing "Tiepi Fengdou" could be successfully differentiated by ARMS ( Fig.…”

“…[9][10][11][12] ARMS (amplification refractory mutation system), which has also been described as allele-specific polymerase chain reaction (PCR), is on the basis of single nucleotide polymorphisms (SNPs) of different sequences and has been successfully used for herbs and their adulterants authentication. 5,13,14) gDNA SSH (genomic DNA suppression subtraction hybridization) is an efficient and sensitive PCR-based gDNA subtraction method and is capable to be applied to germplasm authentication, genetic mapping and gene tagging, molecular marker-assisted breeding and species identification. 15,16) In the present study, sequences of chloroplast genes (matK and rbcL), nuclear rDNA ITS regions, mitochondria gene nad1 intron2 would be analyzed and diagnostic primers for ARMS would be designed for population authentication.…”

The Relationship of Fas Promoter Polymorphisms and Breast Cancer Risk in North-West of Iran: A Haplotype and in Silico Analysis

“…Genotyping of fas-1377G > A and fas-670A > G was done, using tetra-amplification refractory mutation system-polymerase chain reaction (Tetra-ARMS-PCR) and restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) (19), respectively.…”

Authentication of the Thai medicinal plants sharing the same common name Rang Chuet: Thunbergia laurifolia, Crotalaria spectabilis, and Curcuma aff. amada by combined techniques of TLC, PCR-RFLP fingerprints, and antioxidant activities

“…PCR-RFLP is one of the most popular DNA-based techniques for species identification in biology, medicine, and food science 16 . The method was successfully used to differentiate Alisma orientale from Alisma species 17 and Bulbus fritillariae from its adulterants 18 and to identify species in anchovy pastes on the market 19 . The aim of the present study was to identify the antioxidant properties of the three 'Rang Chuet' species, including T. laurifolia, Crotalaria spectabilis, and Curcuma aff.…”