Molecular Assessment of Genetic Diversity and Relationship in Selected Mungbean Germplasms

Bhuyan, Saiful Islam; Hossain, Md. Sanower; Islam, M. M.; Begum, Shameem Ara; Urbi, Zannat; Hossain, Md. Sumon

doi:10.3923/biotech.2014.126.134

Cited by 4 publications

(1 citation statement)

References 31 publications

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“…DNA markers, such as ISSR and RAPD, proved as excellent sources of diversity and establishing new genetic relationships (Randhawa et al, 2013;Etminan et al, 2016;Fathy et al, 2021). RAPD use is one of the quickest and easiest markers to discriminate Rhizobia on strains level (Rajasundari et al, 2009;Shoukry et al, 2013;Bhuyan et al, 2014;Flores-Félix et al, 2020). Meanwhile, ISSR is another good marker for evaluating genetic diversity, characterized by high polymorphism and recurrence (Zhang et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Phenotypic and Genetic Diversity of Native Rhizobium Isolated From Root Nodules of Leguminous Plants Grown in Reclaimed Soil, Egypt

MANSOUR¹,

ELHALOOS²,

ABDEL-LATEIF³

2023

SABRAOJBG

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An investigation of the phenotypic characteristics of 19 rhizobial strains, isolated from root nodules of different plant legumes grown in the soil of agriculture farms in Ismailia governorate, transpired. Most isolates were creamy or white opaque, mucoid, with a convex elevation, translucent, and smooth margined. Microscopic investigation revealed that all bacterial isolates were rod-shaped and had no positive affinity for Gram-stain. Identifying rhizobial cultures from any bacterial contaminants employed confirmatory tests based on prepared special media, including YMA supplement with Congo red, glucose peptone agar, Kit-lactose agar, and Hoffer’s alkaline test. Based on an infectivity test, all isolates proved their ability to reinfect their host. These rhizobial isolates, classified into two categories, included fast and slow-growing rhizobia according to their growth in the YEM medium containing bromothymol blue (BTB). Meanwhile, the assessment of the genetic diversity among these isolates proceeded using ISSR and RAPD markers, which ISSR marker proved a more powerful tool in discriminating among the tested isolates than the RAPD marker. The cluster analysis, with the RAPD marker, classified the isolates into two main groups. The first group included the isolates (1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, and 13), while the second group contained the isolates (12, 14, 15, 16, 17, 18, and 19). Moreover, using ISSR markers also showed a cluster of two main groups with diverse categories; the first cluster included isolates 1 to 11, and the second group contained isolates 12 to 19.

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