Phenotypic and Genetic Diversity of Native Rhizobium Isolated From Root Nodules of Leguminous Plants Grown in Reclaimed Soil, Egypt

MANSOUR, SR; ELHALOOS, BA; ABDEL-LATEIF, KS

doi:10.54910/sabrao2023.55.2.7

Cited by 3 publications

(2 citation statements)

References 25 publications

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“…Both rhizobia and NRBEs from nodules have been isolated from various legume species including wild legumes, fenugreek, lupine, groundnut, peanut, etc. [ 4 , 5 , 6 , 7 , 8 , 9 ]. Several genera of NRBEs other than rhizobia were isolated including Agrobacterium , Bacillus , Enterobacter , Paenibacillus , Erwinia , Aerobacter , Herbaspirillum , Klebsiella , Pantoea , and Pseudomonas [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Bacterial Endophytes from Small Nodules of Field-Grown Peanut

Hossain,

Frith,

Bhattacharyya

et al. 2023

Microorganisms

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It is evident that legume root nodules can accommodate rhizobial and non-rhizobial bacterial endophytes. Our recent nodule microbiome study in peanuts described that small nodules can harbor diverse bacterial endophytes. To understand their functional role, we isolated 87 indigenous endophytes from small nodules of field-grown peanut roots and characterized them at molecular, biochemical, and physiological levels. The amplified 16S rRNA genes and phylogenetic analysis of these isolates revealed a wide variety of microorganisms related to the genera Bacillus, Burkholderia, Enterobacter, Herbaspirillum, Mistsuaria, Pantoea, Pseudomonas, and Rhizobia. It was observed that 37% (100% identity) and 56% (>99% identity) of the isolates matched with the amplified sequence variants (ASVs) from our previous microbiome study. All of these isolates were tested for stress tolerance (high temperature, salinity, acidic pH) and phosphate (P) solubilization along with ammonia (NH3), indole-3-acetic acid (IAA), 1-aminocyclopropane-1-carboxylate deaminase (ACCD), and siderophore production. The majority (78%) of the isolates were found to be halotolerant, thermotolerant, and acidophilic, and a few of them showed a significant positive response to the production of IAA, NH3, siderophore, ACCD, and P-solubilization. To evaluate the plant growth promotion (PGP) activity, plant and nodulation assays were performed in the growth chamber conditions for the selected isolates from both the non-rhizobial and rhizobial groups. However, these isolates appeared to be non-nodulating in the tested conditions. Nonetheless, the isolates 2 (Pantoea), 17 (Burkholderia), 21 (Herbaspirillum), 33o (Pseudomonas), and 77 (Rhizobium sp.) showed significant PGP activity in terms of biomass production. Our findings indicate that these isolates have potential for future biotechnological applications through the development of biologicals for sustainable crop improvement.

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Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Bacterial Endophytes from Small Nodules of Field-Grown Peanut

Hossain,

Frith,

Bhattacharyya

et al. 2023

Microorganisms

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“…These bacteria include the genera, i.e., Rhizobium, Bradyrhizobium, Mesorhizobium, Sinorhizobium, and Allorhizobium, collectively called rhizobia and belonging to the family Rhizobiaceae that fix atmospheric nitrogen by forming nodules in the roots of leguminous crop plants (Pervin et al, 2017). Rhizobiaceae is Gram-negative, aerobic, and selectively organotrophic (Setu et al, 2019;Mansour et al, 2023;Smirnova et al, 2023). Bacillus, in shape, and non-spores forming, are usually found in soil individually or in pairs, and move with circumferential and polar flagella (Hisyan and Al-Shakarchi, 2021).…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity and Phylogeny of Rhizobia Isolated From Nodules Using RFLP-PCR Technique in Nineveh Province, Iraq

AL-SHAKARCHI

2023

SABRAOJBG

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Rhizobia are important bacteria, playing a vital role in atmospheric nitrogen fixation. In addition, it increases soil fertility, enhancing crop production and preserving the soil from pollution resulting from inorganic fertilizers’ use. From 81 bacteria samples, nine isolates segregated from nine types of leguminous crops are economically valuable for cultivation within nine different locations in Nineveh Governorate, Iraq. The isolates underwent initial diagnosis by phenotypical and cultural characteristics and host specialization tests. Molecular characterization also ran on the selected bacterial isolates. Performing the 16S rRNA region amplification applied forward primer 27F and a reverse primer 1541R using the polymerase chain reaction (PCR) technique. The results of restriction fragment length polymorphism (RFLP-PCR) employing three endonuclease enzymes showed that EcoRI had similar sequences among the isolates under study but significantly different for MspI and HaeIII. Based on the analysis of results using the statistical program MVSP version 3.22, the degree of similarity divided the isolates into three groups. Results revealed that there were genetic differences between the isolates and the suppressing enzymes, providing more accurate results in recognizing the similarity among the bacterial isolates as a result of the difference in the location and environmental conditions from which the bacteria were isolated, in addition to the different agricultural processes used for those regions.

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Characterization and screening for inoculation of rhizobium and PGPR for improving growth of mash bean under axenic conditions

Asif,

Asghar,

Zahir

et al. 2023

Preprint

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Excessive use of synthetic fertilizers is a major bottleneck towards adopting microbial techniques on a large scale and poses great challenge to food security by disrupting soil properties and increasing production costs. Current research must focus on developing suitable alternatives for optimizing crop production along with reduced agricultural costs. We compared the efficacy of different rhizobial and rhizobacterial isolates for promoting growth of Vigna mungo in a growth room trial. Results revealed that among PGPR isolates, ‘Stenotrophomonas rhizophila PKA-18’ significantly enhanced shoot length (1.58 fold), root length (95%), root fresh weight (1.45 fold), shoot dry weight (6.3 fold) and SPAD value (1.35 fold) as compared to control. Among rhizobia, inoculation of ‘Rhizobium sp. RKA-47’ significantly increased shoot length (2.07 fold), root length (77%), shoot fresh weight (2.95 fold) and shoot dry weight (1.55 fold). Principal component analysis (PCA) indicated that among rhizobacteria, ‘Bacillus subtilis PKA-25’ and ‘Stenotrophomonas rhizophila PKA-18’ whereas, among rhizobia, ‘Rhizobium sp. RKA-47’ and ‘Bradyrhizobium japonicum RKA-46’ were the most efficient strains. Similarly, root parameters (root diameter, number of tips and root length) with root scanner were noted. Hence, co-inoculation of rhizobacteria and rhizobia proved to be an ideal option for improving mash bean growth under axenic conditions.

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Phenotypic and Genetic Diversity of Native Rhizobium Isolated From Root Nodules of Leguminous Plants Grown in Reclaimed Soil, Egypt

Cited by 3 publications

References 25 publications

Isolation and Characterization of Bacterial Endophytes from Small Nodules of Field-Grown Peanut

Isolation and Characterization of Bacterial Endophytes from Small Nodules of Field-Grown Peanut

Genetic Diversity and Phylogeny of Rhizobia Isolated From Nodules Using RFLP-PCR Technique in Nineveh Province, Iraq

Characterization and screening for inoculation of rhizobium and PGPR for improving growth of mash bean under axenic conditions

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