Molecular approaches to pathogenesis study of Burkholderia cenocepacia, an important cystic fibrosis opportunistic bacterium

Bazzini, Silvia; Udine, Claudia; Riccardi, Giovanna

doi:10.1007/s00253-011-3616-5

Cited by 15 publications

(12 citation statements)

References 80 publications

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“…Burkholderia cenocepacia J2315 is a gram-negative bacterium that causes life threatening infections in cystic fibrosis patients (40). The intracellular replication kinetics in THP-1 cells is known (41).…”

Section: Resultsmentioning

confidence: 99%

Activator of G-Protein Signaling 3–Induced Lysosomal Biogenesis Limits Macrophage Intracellular Bacterial Infection

Vural

Khodor

Cheung

et al. 2016

The Journal of Immunology

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Many intracellular pathogens cause disease by subverting macrophage innate immune defense mechanisms. Intracellular pathogens actively avoid delivery to, or directly target lysosomes, the major intracellular degradative organelle. Here, we demonstrate that AGS3 (Activator of G-protein Signaling 3), a lipopolysaccharide inducible protein in macrophages, affects both lysosomal biogenesis and activity. AGS3 binds the Gi family of G proteins via its G-protein regulatory (GPR/GoLoco) motif stabilizing the Gα subunit in its GDP-bound conformation. Elevated AGS3 levels in macrophages limited the activity of the mammalian target of rapamycin (mTOR) pathway, a sensor of cellular nutritional status. This triggered the nuclear translocation of transcription factor EB (TFEB), a known activator of lysosomal gene transcription. In contrast, AGS3 deficient macrophages had increased mTOR activity, reduced TFEB activity, and a lower lysosomal mass. High levels of AGS3 in macrophages enhanced their resistance to infection by Burkholderia cenocepacia J2315, Mycobacterium tuberculosis, and methicillin-resistant Staphylococcus aureus while AGS3 deficient macrophages were more susceptible. We conclude that LPS priming increases AGS3 levels, which enhances lysosomal function and increases the capacity of macrophages to eliminate intracellular pathogens.

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Section: Resultsmentioning

confidence: 99%

Activator of G-Protein Signaling 3–Induced Lysosomal Biogenesis Limits Macrophage Intracellular Bacterial Infection

Vural

Khodor

Cheung

et al. 2016

The Journal of Immunology

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“…The newly described genera of ticks, such as Rhipicephalus and Amblyomma , are involved in animal ear inflammation, possibly necrotic [44–46]. Streptomyces can cause infections in humans, such as mycetome [47] and Burkholderia cenocepacia is described as being involved in “cepacia syndrome”, characterized by, among others symptoms, uncontrolled bronchopneumonia [48]. In addition, some fungi identified in this work with SMase D activities are plant pathogens, such as Gibberela, Fusarium, Metarhizium and Passalora [49–51].…”

Section: Discussionmentioning

confidence: 99%

Identification of New Sphingomyelinases D in Pathogenic Fungi and Other Pathogenic Organisms

Dias-Lopes

Neshich

et al. 2013

PLoS ONE

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Sphingomyelinases D (SMases D) or dermonecrotic toxins are well characterized in Loxosceles spider venoms and have been described in some strains of pathogenic microorganisms, such as Corynebacterium sp. After spider bites, the SMase D molecules cause skin necrosis and occasional severe systemic manifestations, such as acute renal failure. In this paper, we identified new SMase D amino acid sequences from various organisms belonging to 24 distinct genera, of which, 19 are new. These SMases D share a conserved active site and a C-terminal motif. We suggest that the C-terminal tail is responsible for stabilizing the entire internal structure of the SMase D Tim barrel and that it can be considered an SMase D hallmark in combination with the amino acid residues from the active site. Most of these enzyme sequences were discovered from fungi and the SMase D activity was experimentally confirmed in the fungus Aspergillus flavus. Because most of these novel SMases D are from organisms that are endowed with pathogenic properties similar to those evoked by these enzymes alone, they might be associated with their pathogenic mechanisms.

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“…We have chosen these bacteria since many strains of the complex are opportunistic human pathogens and represent a serious concern for cystic fibrosis (CF) patients and immunocompromised individuals [37], responsible for the “cepacia syndrome,” characterized by high fever, severe progressive respiratory failure, leukocytosis, and elevated erythrocyte sedimentation rate. Moreover, Bcc strains are (naturally) resistant to many antibiotics such as cephalosporin, β -lactams, polymyxins, and aminoglycosides; therefore, Bcc infections are very problematic to eradicate [37, 38]. In spite of this high degree of resistance to many antibiotics, it has been recently demonstrated that essential oils extracted from six medicinal plants are able to completely inhibit the growth of Bcc members, including those with a clinical origin and exhibiting resistance to many antibiotics [39].…”

Section: Introductionmentioning

confidence: 99%

Linking Bacterial Endophytic Communities to Essential Oils: Clues fromLavandula angustifoliaMill

Emiliani

Mengoni

Maida

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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Endophytic bacteria play a crucial role in plant life and are also drawing much attention for their capacity to produce bioactive compounds of relevant biotechnological interest. Here we present the characterisation of the cultivable endophytic bacteria of Lavandula angustifolia Mill.—a species used since antiquity for its therapeutic properties—since the production of bioactive metabolites from medical plants may reside also in the activity of bacterial endophytes through their direct production, PGPR activity on host, and/or elicitation of plant metabolism. Lavender tissues are inhabited by a tissue specific endophytic community dominated by Proteobacteria, highlighting also their difference from the rhizosphere environment where Actinobacteria and Firmicutes are also found. Leaves' endophytic community resulted as the most diverse from the other ecological niches. Overall, the findings reported here suggest: (i) the existence of different entry points for the endophytic community, (ii) its differentiation on the basis of the ecological niche variability, and (iii) a two-step colonization process for roots endophytes. Lastly, many isolates showed a strong inhibition potential against human pathogens and the molecular characterization demonstrated also the presence of not previously described isolates that may constitute a reservoir of bioactive compounds relevant in the field of pathogen control, phytoremediation, and human health.

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Molecular approaches to pathogenesis study of Burkholderia cenocepacia, an important cystic fibrosis opportunistic bacterium

Cited by 15 publications

References 80 publications

Activator of G-Protein Signaling 3–Induced Lysosomal Biogenesis Limits Macrophage Intracellular Bacterial Infection

Activator of G-Protein Signaling 3–Induced Lysosomal Biogenesis Limits Macrophage Intracellular Bacterial Infection

Identification of New Sphingomyelinases D in Pathogenic Fungi and Other Pathogenic Organisms

Linking Bacterial Endophytic Communities to Essential Oils: Clues fromLavandula angustifoliaMill

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